Characterization of plant lectins for their ability to isolate Mycobacterium avium subsp. paratuberculosis from milk

Hobmaier, Bernhard; Lutterberg, Karina; Kleinwort, Kristina J. H.; Mayer, Ricarda; Hirmer, Sieglinde; Amann, Barbara; Hölzel, Christina; Märtlbauer, Erwin; Deeg, Cornelia A.

doi:10.1016/j.fm.2019.02.009

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…Another focus of our study was on identifying possible candidate proteins for new detection methods for MAP, as there is still a lack of a rapid and sensitive detection method for MAP [ 70 ]. The gold standard for MAP detection is currently cultivation on agar [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Kleinwort¹,

Hobmaier²,

Mayer

et al. 2021

Metabolites

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Mycobacterium avium subspecies paratuberculosis (MAP) are detectable viable in milk and other dairy products. The molecular mechanisms allowing the adaptation of MAP in these products are still poorly understood. To obtain information about respective adaptation of MAP in milk, we differentially analyzed the proteomes of MAP cultivated for 48 h in either milk at 37 °C or 4 °C or Middlebrook 7H9 broth as a control. From a total of 2197 MAP proteins identified, 242 proteins were at least fivefold higher in abundance in milk. MAP responded to the nutritional shortage in milk with upregulation of 32% of proteins with function in metabolism and 17% in fatty acid metabolism/synthesis. Additionally, MAP upregulated clusters of 19% proteins with roles in stress responses and immune evasion, 19% in transcription/translation, and 13% in bacterial cell wall synthesis. Dut, MmpL4_1, and RecA were only detected in MAP incubated in milk, pointing to very important roles of these proteins for MAP coping with a stressful environment. Dut is essential and plays an exclusive role for growth, MmpL4_1 for virulence through secretion of specific lipids, and RecA for SOS response of mycobacteria. Further, 35 candidates with stable expression in all conditions were detected, which could serve as targets for detection. Data are available via ProteomeXchange with identifier PXD027444.

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Section: Discussionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Kleinwort¹,

Hobmaier²,

Mayer

et al. 2021

Metabolites

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“…In clinically infected cattle it causes weight loss, diarrhea, and reduced milk yields, and therefore causes major economic losses for the farmer [ 3 , 7 ]. After infection, ruminants usually go through a long, asymptomatic subclinical phase during which MAP cannot reliably be detected by standard diagnostic tests [ 8 , 9 ]. These infected but unrecognized animals are the major spreaders of the disease in livestock and remain difficult to identify because available diagnostic tests are not sensitive enough to detect them [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

et al. 2022

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Bovine paratuberculosis is a serious chronic disease of the gastrointestinal tract that causes economic losses and dramatically affects animal health in livestock. The underlying infectious agent, Mycobacterium avium subspecies paratuberculosis (MAP), cannot reliably be detected by standard diagnostic tests due to the long asymptomatic disease stage. The aim of this study was to detect proteomic changes in peripheral blood mononuclear cells (PBMC) from cows of the same herd with different MAP infection status after co-incubation with viable MAP in vitro using label-free LC-MS/MS. In our proteomic discovery experiment, we detected 2631 differentially regulated proteins between cows with negative MAP infection status (so-called MAP-resistant cows) and cows with positive MAP infection status (so-called persistently MAP-infected cows). In MAP-resistant cows, we detected enriched immune-related signaling pathways for TLR2 and MHC class II component proteins, among others, indicating a successful defensive immune response of the cows to MAP. In contrast, persistently MAP-infected cows were not directly enriched in immune-related signaling pathways associated with ITGA2B and KCNMA1, among others. The introduction of these distinct immune responses contributes to a better understanding of the bovine immune response and mechanisms of susceptibility to MAP.

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“…Paratuberculosis, also referred to as Johne’s disease, is endemic world-wide endemic; no country or region has been found to be free of this disease (Li et al, 2016). Affected ruminants go through a long asymptomatic subclinical phase in which infection cannot reliably be detected by standard diagnostic tests (Hobmaier et al, 2019; Li et al, 2017). These subclinically infected animals can already shed or harbor MAP and thereby contaminate dairy products or meat (Sweeney, 2011).…”

Section: Introductionmentioning

confidence: 99%

Peripheral blood bovine lymphocytes and MAP show distinctly different proteome changes and immune pathways in host-pathogen interaction

Kleinwort¹,

Hauck

Degroote³

et al. 2019

PeerJ

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Mycobacterium avium subsp. paratuberculosis (MAP) is a pathogen causing paratuberculosis in cattle and small ruminants. During the long asymptomatic subclinical stage, high numbers of MAP are excreted and can be transmitted to food for human consumption, where they survive many of the standard techniques of food decontamination. Whether MAP is a human pathogen is currently under debate. The aim of this study was a better understanding of the host-pathogen response by analyzing the interaction of peripheral blood lymphocytes (PBL) from cattle with MAP in their exoproteomes/secretomes to gain more information about the pathogenic mechanisms of MAP. Because in other mycobacterial infections, the immune phenotype correlates with susceptibility, we additionally tested the interaction of MAP with recently detected cattle with a different immune capacity referred as immune deviant (ID) cows. In PBL, different biological pathways were enhanced in response to MAP dependent on the immune phenotype of the host. PBL of control cows activated members of cell activation and chemotaxis of leukocytes pathway as well as IL-12 mediated signaling. In contrast, in ID cows CNOT1 was detected as highly abundant protein, pointing to a different immune response, which could be favorable for MAP. Additionally, MAP exoproteomes differed in either GroEL1 or DnaK abundance, depending on the interacting host immune response. These finding point to an interdependent, tightly regulated response of the bovine immune system to MAP and vise versa.

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Characterization of plant lectins for their ability to isolate Mycobacterium avium subsp. paratuberculosis from milk

Cited by 7 publications

References 47 publications

Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

Peripheral blood bovine lymphocytes and MAP show distinctly different proteome changes and immune pathways in host-pathogen interaction

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