Characterization of Plant-Based Biotin from SesZenBioTM and In silico Studies to Prove Its Potential in Hair Care

Abstract

A standardized extract from Sesbania agati leaves (SESAL) was developed by Zenherb labs Pvt. Ltd., Mumbai, and is one of its kind plant-based biotin supplements. The current work aimed at characterization of SESAL using chromatographic techniques (like HPLC & HPTLC) and DNA authentication. Additionally, an in-silico approach (docking) was also adopted to predict the molecular interactions and probable mechanisms involved in potential role of SESAL in hair care products range. Drug likeliness was assessed on the basis of ADMET properties and Lipinski’s Rule of 5. DNA fingerprinting followed by HPTLC confirmed the identification and authentication. The biotin content in SesZenBio was estimated to be 0.5% by validated HPLC method. Mechanistically, biotin along with 2 other cofactors in SesZenBioTM is predicted to stimulate the hair follicle growth and differentiation, thus improving hair health. SesZenBioTM might hold 2x potential as against synthetic biotin in hair care applications.

A prospective, randomized double-blind, placebo-controlled study for safety and efficacy of SesZen-Bio™: a proprietary Sesbania grandiflora extract in managing hair and scalp health in healthy adults

“…In silico testing suggested that test treatment enhances hair follicle growth and improve hair health. 19 In a survey of 900 physicians, including 300 dermatologists, 66% of dermatologists stated recommending dietary supplements to their patients to improve their skin and hair health. 23 Hair loss conditions are multifactorial, ranging from improper nutrition, environmental factors causing stress to hormonal imbalances, inflammation, drug use and many more.…”

“…[16][17][18] The leaves and flowers of Agati are abundant in vitamins (biotin, A, C, B2, etc) and minerals (zinc and selenium), whereas the bark and flowers are rich in polyphenols. 19 Biotin, alternatively recognized as Vitamin B7 or H (with H derived from "Haar und Haut," German for "hair and skin"), is a water-soluble B vitamin serving as a crucial cofactor for numerous carboxylases which play pivotal role in the cellular metabolism of fatty acids, amino acids, and gluconeogenesis. 20 Due to its affordability and widespread availability, biotin has emerged as an effective solution for hair health, promoting longer and healthier growth.…”

“…In silico studies suggest that it is twice as effective as synthetic biotin in hair care. 19,22 This study was conducted to gain deeper insights into the safety, efficacy, and utilization of test treatment (SesZen-Bio ® ) vs placebo in healthy subjects with poor hair (thin, dry and brittle) and alopecia.…”