Characterization of PIR1, a GATA family transcription factor involved in iron responses in the white-rot fungus Phanerochaete chrysosporium

Canessa, Paulo; Muñoz-Guzmán, Felipe; Vicuña, Rafael; Larrondo, Luis F.

doi:10.1016/j.fgb.2012.05.013

Cited by 3 publications

(3 citation statements)

References 65 publications

(102 reference statements)

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“…For example, adding 56 µg/mL of iron could promote the growth and sporulation capacity of Cylindrocarpon destructans [ 18 ]. The growth rate and sporulation capacity of Phanerochaete chrysosporium could be significantly improved by the appropriate concentration of iron ions [ 19 ]. Iron balance is beneficial for promoting and enhancing the germination and infectivity of Beauveria bassiana spores [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation of Antrodia cinnamomea in Submerged Fermentation Revealed by Comparative Transcriptomics

Dai

Shi

et al. 2023

JoF

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Antrodia cinnamomea is a precious edible and medicinal fungus with activities of antitumor, antivirus, and immunoregulation. Fe2+ was found to promote the asexual sporulation of A. cinnamomea markedly, but the molecular regulatory mechanism of the effect is unclear. In the present study, comparative transcriptomics analysis using RNA sequencing (RNA-seq) and real time quantitative PCR (RT-qPCR) were conducted on A. cinnamomea mycelia cultured in the presence or absence of Fe2+ to reveal the molecular regulatory mechanisms underlying iron-ion-promoted asexual sporulation. The obtained mechanism is as follows: A. cinnamomea acquires iron ions through reductive iron assimilation (RIA) and siderophore-mediated iron assimilation (SIA). In RIA, ferrous iron ions are directly transported into cells by the high-affinity protein complex formed by a ferroxidase (FetC) and an Fe transporter permease (FtrA). In SIA, siderophores are secreted externally to chelate the iron in the extracellular environment. Then, the chelates are transported into cells through the siderophore channels (Sit1/MirB) on the cell membrane and hydrolyzed by a hydrolase (EstB) in the cell to release iron ions. The O-methyltransferase TpcA and the regulatory protein URBS1 promote the synthesis of siderophores. HapX and SreA respond to and maintain the balance of the intercellular concentration of iron ions. Furthermore, HapX and SreA promote the expression of flbD and abaA, respectively. In addition, iron ions promote the expression of relevant genes in the cell wall integrity signaling pathway, thereby accelerating the cell wall synthesis and maturation of spores. This study contributes to the rational adjustment and control of the sporulation of A. cinnamomea and thereby improves the efficiency of the preparation of inoculum for submerged fermentation.

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Section: Introductionmentioning

confidence: 99%

Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation of Antrodia cinnamomea in Submerged Fermentation Revealed by Comparative Transcriptomics

Dai

Shi

et al. 2023

JoF

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“…Fe could generate reactive oxygen species with a high destructive capacity for straw decomposition due to the redox properties [18]. Fe is also a critical cofactor for major lignin-degrading enzymes including lignin peroxidase (Lip) and manganese peroxidase (Mnp) in white-rot fungi, and also plays a key role in Fenton reactions [19]. The latter are essential for brownrotter fungi to depolymerize cellulose.…”

Section: Introductionmentioning

confidence: 99%

“…The latter are essential for brownrotter fungi to depolymerize cellulose. Multicopper oxidases with ferroxidase activity have been implicated in regulating Fe bioavailability in the hyphal proximity, fine-tuning Fenton chemistry, and balancing lignin vs. cellulose decomposition [19]. Previous works have reported contradictory results in regards to the impact of Fe addition on crop straw decomposition.…”

Section: Introductionmentioning

confidence: 99%

High Level of Iron Inhibited Maize Straw Decomposition by Suppressing Microbial Communities and Enzyme Activities

et al. 2022

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In order to study the linkages between the crop straw decomposition rate and the change in soil biological properties after the straw returned to the soil with different iron (Fe2+) contents, a 180-day incubation experiment was performed to examine the decomposition of maize straw (MS) under three Fe2+ levels, i.e., 0, 0.3, and 1 mg g−1. Enzyme activities regarding straw decomposition and microbial communities under 0 and 1 mg g−1 Fe addition were also detected. The results showed that Fe2+ addition significantly inhibited MS decomposition. This was evidenced by the higher contents of hemicellulose, cellulose, and lignin in Fe2+ treatments on day 180. High-Fe addition (1 mg g−1) decreased the activity of Laccase (Lac) by 71.82% compared with control on day 30. Furthermore, the principal coordinates analysis (PCoA) indicated that high-Fe mainly affected the bacterial community. In particular, it suppressed the relative abundance of Microbacteriaceae in phylum Actinomycota that, in turn, is a potential decomposer of crop straw by secreting lignocellulolytic enzymes. A high level of Fe2+ inhibited the decomposition of hemicellulose, cellulose, and lignin in MS by reducing the relative abundance of phylum Actinobacteria in bacteria and suppressing Lac activity. Our findings provide guidance for returning crop straws in soils with high-Fe content.

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Environmental responses and the control of iron homeostasis in fungal systems

Canessa

Larrondo

2012

Appl Microbiol Biotechnol

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Organisms need to actively respond to changes in the environment and, particularly under diverse conditions, they ought to ensure access to nutrients. Among micronutrients, iron is a key component of several enzymes and participates in a variety of cellular processes. Iron deprivation therefore poses a serious challenge to both unicellular and multicellular individuals. Nevertheless, excess of this metal is toxic, compromising cell function and viability. Thus, it is not surprising that organisms have evolved sophisticated mechanisms to tightly regulate cellular iron levels. In the last decade, major advances have been achieved in the molecular understanding of how fungi respond to changing iron concentrations. Moreover, this metal has been recognized as an important element impacting pathogenic and saprophytic fungal lifestyles. An interconnected transcriptional negative feedback loop has been described as central in the regulation of genes encoding for iron uptake and utilization components in fungi. The observation that light, oxygen, or nutrients can also impact the expression of some of these elements suggests that additional environmental inputs-besides iron levels-may as well modulate the machinery underpinning iron homeostasis. This review highlights some of the latest findings associated with iron-regulated processes in fungi and revisits the increasing transcriptional complexity involved in the control of this metal homeostasis. In addition, we present the first in silico evidence of genes encoding for putative ferritins in zygomycetes and chytrids, as well as other ferritin-like sequences widespread among fungi, which raises interesting questions relative to iron storage in this particular group of organisms.

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Characterization of PIR1, a GATA family transcription factor involved in iron responses in the white-rot fungus Phanerochaete chrysosporium

Cited by 3 publications

References 65 publications

Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation of Antrodia cinnamomea in Submerged Fermentation Revealed by Comparative Transcriptomics

Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation of Antrodia cinnamomea in Submerged Fermentation Revealed by Comparative Transcriptomics

High Level of Iron Inhibited Maize Straw Decomposition by Suppressing Microbial Communities and Enzyme Activities

Environmental responses and the control of iron homeostasis in fungal systems

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