Characterization of pigment producing rhodotorula from dairy environmental samples

Yadav, Shivalkar; Manjunatha, K.H.; Ramachandra, B.; Suchitra, N.; Prabha, Ratna

doi:10.5958/j.0976-0563.33.1.001

Cited by 7 publications

(2 citation statements)

References 4 publications

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“…Sequences revealed the presence of Rhodotorula graminis , and only a few reads were classified as Candida spp . Rhodotorula is a carotenoid biosynthetic yeast, part of the Basidiomycota phylum, easily identifiable by distinctive yellow, orange or red colonies [ 28 ]. This yeast produces three major carotenoids: b-carotene, torulene and torularhodin, and is commonly associated with plants ( http://www.antimicrobe.org/f16.asp#t1 ).…”

Section: Resultsmentioning

confidence: 99%

Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples

D’Andreano

Cuscó²,

Francino

2020

Biology Methods and Protocols

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The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S) and to improve the taxonomy assignment of the communities up to species level and in real-time. We assess the applicability for taxonomic assignment of amplicons targeting a 3.5 Kb region (V3 18S-ITS1-5.8S-ITS2-28S D2) and a 6 Kb region (V1 18S-ITS1-5.8S-ITS2-28S D12) with the What’s in my pot (WIMP) classifier. We used the ZymoBIOMICSTM mock community and different microbiological fungal cultures as positive controls. Long amplicon sequencing correctly identified Saccharomyces cerevisiae and Cryptococcus neoformans from the mock community and Malassezia pachydermatis, Microsporum canis and Aspergillus fumigatus from the microbiological cultures. Besides, we identified Rhodotorula graminis in a culture mislabelled as Candida spp. We applied the same approach to external otitis in dogs. Malassezia was the dominant fungal genus in dogs’ ear skin, whereas Ma. pachydermatis was the main species in the healthy sample. Conversely, we identified a higher representation of Ma. globosa and Ma. sympodialis in otitis affected samples. We demonstrate the suitability of long ribosomal amplicons to characterize the fungal community of complex samples, either healthy or with clinical signs of infection.

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Section: Resultsmentioning

confidence: 99%

Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples

D’Andreano

Cuscó²,

Francino

2020

Biology Methods and Protocols

View full text Add to dashboard Cite

show abstract

“…Sequences revealed the presence of Rhodotorula graminis, and only a few reads were classified as Candida spp. Rhodotorula is a carotenoid biosynthetic yeast, part of the Basidiomycota phylum, easily identifiable by distinctive yellow, orange or red colonies (Yadav et al 2014). This yeast produces three major carotenoids: b-carotene, torulene and torularhodin, and is commonly associated with plants (http://www.antimicrobe.org/f16.asp#t1).…”

Section: Identification Of Microbiological Cultures and Mock Communitmentioning

confidence: 99%

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

D’Andreano

Cuscó

Francino

2020

Preprint

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ABSTRACTThe availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S), and to improve the taxonomy assignment of the communities up to the species level and in real-time. We assess the taxonomy skills of amplicons targeting a 3.5 Kb region (V3 18S-ITS1-5.8S-ITS2-28S D2) and a 6 Kb region (V1 18S-ITS1-5.8S-ITS2-28S D12) with the What’s in my pot (WIMP) classifier. We used the ZymoBIOMICS™ mock community and different microbiological fungal cultures as positive controls. Long amplicon sequencing correctly identified Saccharomyces cerevisiae and Cryptococcus neoformans from the mock community and Malassezia pachydermatis, Microsporum canis, and Aspergillus fumigatus from the microbiological cultures. Besides, we identified Rhodotorula graminis in a culture mislabeled as Candida spp.We applied the same approach to external otitis in dogs. Malassezia was the dominant fungal genus in dogs’ ear skin, whereas M. pachydermatis was the main species in the healthy sample. Conversely, we identified a higher representation of M. globosa and M. sympodialis in otitis affected samples. We demonstrate the suitability of long ribosomal amplicons to characterize the fungal community of complex samples, either healthy or with clinical signs of infection.

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Therapeutic applications of bacterial pigments: a review of current status and future opportunities

et al. 2018

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Non-toxicity, biodegradability and non-carcinogenicity of the natural pigments, dyes and colorants make them an attractive source for human use. Bacterial pigments are colored metabolites secreted by bacteria under stress. The industrial uses of bacterial pigments have increased many folds because of several advantages over the synthetic pigments. Among natural resources, bacterial pigments are mostly preferred because of simple culturing and pigment extraction techniques, scaling up and being time economical. Generally, the bacterial pigments are safe for human use and therefore have a wide range of applications in pharmaceutical, textile, cosmetics and food industries. Therapeutic nature of the bacterial pigments is revealed because of their antimicrobial, anticancer, cytotoxic and remarkable antioxidant properties. Owing to the importance of bacterial pigments it was considered important to produce a comprehensive review of literature on the therapeutic and industrial potential of bacterial pigments. Extensive literature has been reviewed on the biomedical application of bacterial pigments while further opportunities and future challenges have been discussed.

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Characterization of pigment producing rhodotorula from dairy environmental samples

Cited by 7 publications

References 4 publications

Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples

Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

Therapeutic applications of bacterial pigments: a review of current status and future opportunities

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