Characterization of Phytochemicals Isolated From Cucurbita Pepo Seeds Using Uv–vis and Ftir Spectroscopy

Rawat, Beena; Garg, Anita

doi:10.51470/plantarchives.2021.v21.no1.122

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Presence of phenol was observed in the peak at 3332.36 cm -1 [31], 3364.58 cm -1 [7], 3270 -3320 cm -1 [43], 3406.43 cm -1 [3]. But in the present study the phenol was found in 1375.40 cm -1 in the gametophyte extract of A. schimperiana subsp.…”

Section: Discussioncontrasting

confidence: 59%

Uv-Vis and Ftir Spectroscopic Profile of Gametophyte and Sporophyte Ethanolic Extract of Anemia Schimperiana C. Presl Subsp. Wightiana (Gardner) Fraser-Jenk. And Cyathea Gigantea (Wall. Ex. Hook.) Holtt.

IRUTHAYAMANI,

JOHNSON

2023

Romanian J. Biophys.

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In the present study, an attempt is made to reveal the functional groups and metabolites of gametophyte and sporophyte of two pteridophytes Anemia schimperiana C. Presl subsp. wightiana (Gardner) Fraser-Jenk. and Cyathea gigantea (Wall ex Hook.) Holtt using UV-Vis and FTIR analysis. For UV-Vis analysis, the ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were centrifuged at 3000 rpm for 5 min and filtered using Whatman No. 1 filter paper. The ethanolic extracts were scanned and recorded using Shimadzu UV-Vis spectrophotometer at the absorbance of 200 to 1100 nm. For FTIR spectrometry about 1.0 mg of gametophyte and sporophyte ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were separately made into very thin discs with potassium bromide and the pellets are measured in an automatic recording IR Spectrophotometer in the range of 4004000 cm1. Based on the observed peak values, the existence of alkaloids, phenolics, tannin and carotenoid existence were confirmed. Terpenoids, flavonoid and chlorophyll existence were also validated in different peak values of the ethanolic extracts of gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The FTIR analysis results showed various functional groups viz., aromatic compounds, alkene, fluoro compounds, anhydride, aliphatic ether, alcohol, secondary amine presence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The gametophyte and sporophyte stages expressed the existence of different functional groups and displayed the variations and similarities between the studied stages. The spectroscopic results confirmed the secondary metabolites existence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea ethanolic extracts. Further chromatographic and separation studies may bring out stage-specific bioactive principles from the gametophytes and sporophytes of studied ferns.

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Section: Discussioncontrasting

confidence: 59%

Uv-Vis and Ftir Spectroscopic Profile of Gametophyte and Sporophyte Ethanolic Extract of Anemia Schimperiana C. Presl Subsp. Wightiana (Gardner) Fraser-Jenk. And Cyathea Gigantea (Wall. Ex. Hook.) Holtt.

IRUTHAYAMANI,

JOHNSON

2023

Romanian J. Biophys.

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“…Spektrofotometri UV-Vis dapat mengidentifikasi jenis senyawa dengan gugus kromofor dan mempunyai ikatan rangkap terkonjugasi. Selain itu, berbagai faktor seperti pH, komposisi larutan, suhu, keberadaan zat pengganggu atau pengotor, dan kebersihan kuvet yang digunakan dalam analisis dapat secara signifikan mempengaruhi hasil pengukuran penyerapan (Kambira et al, 2020;Rawat & Garg, 2021).…”

Section: Spektrofotometri Uv-visunclassified

Review artikel: metode analisis dalam pengujian kandungan pewarna merah K10 (Rhodamin B) pada kosmetik

Kamilania,

Husni

2023

JPS

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Kosmetik merupakan produk yang digunakan untuk memperbaiki penampilan tubuh bagian luar. Kosmetik tidak terlepas dari pewarna, salah satu pewarna yang sering disalahgunakan penggunaannya dalam produk kosmetik adalah Rhodamin B. Rhodamin B dapat menyebabkan kerusakan hati, iritasi pada saluran pernapasan, dan bersifat karsinogenik. Tujuan dari review artikel ini adalah untuk memberikan informasi tentang penggunaan teknik analisis untuk mengidentifikasi produk kosmetik yang mengandung rhodamin B. Metode yang digunakan yaitu literature review dengan mencari jurnal penelitian dengan rentang waktu 2013- 2023 pada Science Direct dan Google Scholar menggunakan kata kunci “identifikasi pewarna merah k10 pada kosmetik”, “analisis rhodamin B pada kosmetik” dan “metode analisis pewarna merah k10 pada kosmetik”. Berdasarkan literature review yang telah dilakukan, untuk mengidentifikasi kandungan pewarna rhodamin B dapat dengan berbagai teknik analisa diantaranya Kromatografi Lapis Tipis (KLT), Kromatografi Cair Kinerja Tinggi (KCKT), Spektrofotometri UV-Vis, Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometric (UPLC-MS), dan rapid test kit. Untuk analisis kualitatif kandungan rhodamin B pada kosmetik dapat dilakukan dengan KLT dan rapid test kit. Sedangkan untuk analisa kuantitatif dapat dilakukan dengan metode spektrofotometri UV-Vis, KCKT, dan UPLC-MS.

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Green Synthesis of Titanium dioxide Nanoparticles by utilizing Marchantia polymorpha and their Application in Methylene Blue Dye Removal

Dadwal,

Kumari,

Nike

et al. 2024

Catal Lett

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Characterization of Phytochemicals Isolated From Cucurbita Pepo Seeds Using Uv–vis and Ftir Spectroscopy

Abstract: CHARACTERIZATION OF PHYTOCHEMICALS ISOLATED FROM CUCURBITA PEPO SEEDS USING UV–VIS AND FTIR SPECTROSCOPY

Cited by 4 publications

References 20 publications

Uv-Vis and Ftir Spectroscopic Profile of Gametophyte and Sporophyte Ethanolic Extract of Anemia Schimperiana C. Presl Subsp. Wightiana (Gardner) Fraser-Jenk. And Cyathea Gigantea (Wall. Ex. Hook.) Holtt.

Uv-Vis and Ftir Spectroscopic Profile of Gametophyte and Sporophyte Ethanolic Extract of Anemia Schimperiana C. Presl Subsp. Wightiana (Gardner) Fraser-Jenk. And Cyathea Gigantea (Wall. Ex. Hook.) Holtt.

Review artikel: metode analisis dalam pengujian kandungan pewarna merah K10 (Rhodamin B) pada kosmetik

Green Synthesis of Titanium dioxide Nanoparticles by utilizing Marchantia polymorpha and their Application in Methylene Blue Dye Removal

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