Characterization of physically different human T-cell subsets with monoclonal antibodies and biochemical markers

Griend, René Van de; Astaldi, A; Bruin, H. de; Tanke, Hans J.; Doorn, R. Van; Meerhof, Louis J.; Roos, Dirk

doi:10.1016/0090-1229(81)90198-7

Cited by 21 publications

(2 citation statements)

References 27 publications

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“…ND = Not done. density were enriched in OKT4-positive (helper) T cells, while lymphocytes with higher density were en riched in OKT8-positive (suppressor/cytotoxic) T cells [19], It thus seems as if mercuric chloride, like mitogenic lectins, is a polyclonal activator of human peripheral T lymphocytes and thymocytes with characteristics of medullary cells (virgin T cells).…”

Section: Discussionmentioning

confidence: 98%

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Nordlind¹

1984

Int Arch Allergy Immunol

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Human thymocytes and peripheral blood lymphocytes were separated on the basis of density on Percoll gradients and the different cell populations tested regarding DNA synthesis after addition of mercuric chloride. Thymocytes with a density of 1.065–1.067 g/ml and peripheral blood lymphocytes with a density of 1.063–1.065 g/ml were maximally stimulated with mercuric chloride as well as with mitogenic lectins. Thus mercuric chloride seems to be a polyclonal activator of human peripheral T lymphocytes and thymocytes with characteristics of medullary cells (virgin T cells).

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Section: Discussionmentioning

confidence: 98%

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Nordlind¹

1984

Int Arch Allergy Immunol

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“…However, functional heterodimeric receptor is acquired first at an early stage in the individual ontogeny by double-negative thymocytes, and such T3'4-8-lymphocytes are also present in human peripheral blood ( [38], manuscript in preparation). Double-negative O+ Lyt-lymphocytes have also been reported in peripheral lymphoid organs of normal mice [39] and in certain T cell malignancies ( [40], unpublished results).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of human mature thymocytes: Existence of a T3⁺4⁻8⁻ intermediate stage

et al. 1986

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A T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. We show differentiation in vitro of both mature subsets of thymocytes (T3+4+8- and T3+4-8+) from the above T4-8- compartment, their appearance being preceded by cells in a T3+4-8- intermediate stage. Furthermore, those mature thymocytes generated in vitro contain functionally competent cells which use T3, T4 and T8 structures for their cytolytic activity. The finding of T3+4-8- thymocytes in vivo, together with the observation that T3 antigen expression precedes that of T4 or T8 molecules in vitro, shows that T3 (and presumably Ti) is present early in ontogeny, and suggests that T3+4-8- cells constitute an "intermediate" stage relevant to the connection between early precursors and mature thymocytes during T lymphocyte ontogeny.

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Purine Metabolism in Human Thymocyte Subsets: Relevance for Lymphocytic Differentiation

Schuurman¹,

Laarhoven

Broekhuizen³

et al. 1984

Advances in Experimental Medicine and Biology

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Characterization of physically different human T-cell subsets with monoclonal antibodies and biochemical markers

Cited by 21 publications

References 27 publications

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Differentiation of human mature thymocytes: Existence of a T3⁺4⁻8⁻ intermediate stage

Purine Metabolism in Human Thymocyte Subsets: Relevance for Lymphocytic Differentiation

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Characterization of physically different human T-cell subsets with monoclonal antibodies and biochemical markers

Cited by 21 publications

References 27 publications

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

Differentiation of human mature thymocytes: Existence of a T3+4−8− intermediate stage

Purine Metabolism in Human Thymocyte Subsets: Relevance for Lymphocytic Differentiation

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Differentiation of human mature thymocytes: Existence of a T3⁺4⁻8⁻ intermediate stage