Characterization of photosystem II in salt-stressed cyanobacterial Spirulina platensis cells

Gong, Hongmei; Tang, Yun; Wang, Jia; Wen, Xiaopeng; Zhang, Lixin; Lu, Congming

doi:10.1016/j.bbabio.2008.03.018

Cited by 55 publications

(29 citation statements)

References 66 publications

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“…In control cells not subjected to nitrite treatment, the B band oscillated with a periodicity of 4, with the maximum emission occurring after the second flash. This is the same type of oscillation as that observed in the cyanobacteria (42,43). In the 6-h period following the addition of excess nitrite, the cells showed a oscillation pattern similar to that seen in the control.…”

supporting

confidence: 62%

“…Our TL data are consistent with the observation of Sahay et al (31) that nitrite inhibited the OEC on the donor side of PSII, as indicated by the suppression of the Q band and the upshift of the B band in nitrite-treated isolated chloroplasts. The increase in the stability of the S 2 state arises from the inactivation of the donor side (43). It has been reported that a mutant lacking the PsbO protein exhibits upshifts in the peak temperatures of S 2 Q A Ϫ and S 2 Q B Ϫ recombination, based on the TL profiles in a cyanobacterium, Synechocystis sp.…”

Section: Discussionmentioning

confidence: 99%

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The Acceptor Side of Photosystem II Is the Initial Target of Nitrite Stress in Synechocystis sp. Strain PCC 6803

Zhang

Zhu

et al. 2017

Appl Environ Microbiol

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Nitrite, a common form of inorganic nitrogen (N), can be used as a nitrogen source through N assimilation. However, high levels of nitrite depress photosynthesis in various organisms. In this study, we investigated which components of the photosynthetic electron transfer chain are targeted by nitrite stress in Synechocystis sp. strain PCC 6803 cells. Measurements of whole-chain and photosystem II (PSII)-mediated electron transport activities revealed that high levels of nitrite primarily impair electron flow in PSII. Changes in PSII activity in response to nitrite stress occurred in two distinct phases. During the first phase, which occurred in the first 3 h of nitrite treatment, electron transfer from the primary quinone acceptor (Q A ) to the secondary quinone acceptor (Q B ) was retarded, as indicated by chlorophyll (Chl) a fluorescence induction, S-state distribution, and Q A Ϫ reoxidation tests. In the second phase, which occurred after 6 h of nitrite exposure, the reaction center was inactivated and the donor side of photosystem II was inhibited, as revealed by changes in Chl fluorescence parameters and thermoluminescence and by immunoblot analysis. Our data suggest that nitrite stress is highly damaging to PSII and disrupts PSII activity by a stepwise mechanism in which the acceptor side is the initial target. IMPORTANCE In our previous studies, an alga-based technology was proposed to fix the large amounts of nitrite that are released from NO X -rich flue gases and proved to be a promising industrial strategy for flue gas NO X bioremediation (W. Chen et al., Environ Sci Technol 50:1620 -1627, https://doi.org/10.1021/ acs.est.5b04696; X. Zhang et al., Environ Sci Technol 48:10497-10504, 2014, https:// doi.org/10.1021/es5013824). However, the toxic effects of high concentrations of nitrite on algal cells remain obscure. The analysis of growth rates, photochemistry, and protein profiles in our study provides important evidence that the inhibition by nitrite occurs in two phases: in the first phase, electron transfer between Q A Ϫ and Q B is retarded, whereas in the second, the donor side of PSII is affected. This is an excellent example of investigating the "early" inhibitory effects (i.e., within the first 6 h) on the PSII electron transfer chain in vivo. This paper provides novel insights into the mechanisms of nitrite inhibition of photosynthesis in an oxygenic phototrophic cyanobacterium.

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supporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

The Acceptor Side of Photosystem II Is the Initial Target of Nitrite Stress in Synechocystis sp. Strain PCC 6803

Zhang

Zhu

et al. 2017

Appl Environ Microbiol

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“…In a study using the cyanobacterium Spirulina, the downregulation of PSII reaction center proteins was observed without any inhibition of electron transfer on the donor and acceptor sides of PSII (Lu and Vonshak 1999), suggesting that the eVects of salt stress on PSII may vary between species. A very recent paper by Gong et al (2008) reports that there were no signiWcant changes in the levels of the CP47, CP43, cytochrome c550, and D1 proteins. The PsbO content in isolated thylakoids, however, decreased.…”

Section: Introductionmentioning

confidence: 96%

Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions

Subramanyam

Jolley

Thangaraj

et al. 2010

Planta

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The eVect of high salt concentration (100 mM NaCl) on the organization of photosystem I-light harvesting complex I supercomplexes (PSI-LHCI) of Chlamydomonas reinhardtii was studied. The electron transfer activity was reduced by 39% in isolated PSI-LHCI supercomplexes. The visible circular dichroism (CD) spectra associated with strongly coupled chlorophyll (Chl) dimers were reduced in intensity, indicating that pigment-pigment interactions were disrupted. This data is consistent with results from Xuorescence streak camera spectroscopy, which suggest that red-shifted pigments in the PSI-LHCI antenna had been lost. Denaturing gel electrophoresis and immunoblot analysis reveals that levels of the PSI reaction center proteins PsaD, PsaE and PsaF were reduced due to salt stress. PsaE is almost completely absent under high salt conditions. It is known that the membrane-extrinsic subunits PsaD and E form the ferredoxin-docking site. Our results indicate that the PSI-LHCI supercomplex is damaged by reactive oxygen species at high salt concentration, with particular impact on the ferredoxin-docking site and the PSILHCI interface.

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“…Chlorophyll fluorescence is commonly used in the study of photosynthesis, particularly PSII function (Schreiber et al 1995). In fact, chlorophyll fluorescence has been extensively used to investigate the photosynthetic characteristics and stress tolerance abilities of land plants and algae (Baker and Rosenquist 2004;Gong et al 2008;Enríquez and Borowitzka 2011). In addition, the photosynthetic pigment contents and ratios are important characteristics of Pyropia cultivars because these characteristics are closely related to product color , and the color is an important factor in determining product price.…”

Section: Introductionmentioning

confidence: 99%

Study on the photosynthetic characteristics of a Pyropia haitanensis red mutant cultured under different light intensities

Zhang

et al. 2015

J Appl Phycol

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The photosynthetic characteristics of a Pyropia haitanensis red mutant mutated from a wild-type strain using chemical mutagens were studied. The aim was to compare the differences in photosynthetic characteristics between the red mutant and the wild type, and then to analyse the possibility of the red mutant as breeding material. The results indicated that the cytoplasm thickness of the red mutant and the wild type were similar, whereas the cell wall of the red mutant was significantly thinner than that of the wild type. Additionally, the chlorophyll a extract absorption spectra of both strains exhibited no obvious difference, whereas the absorption peaks at 498, 545, and 565 nm (which belong to phycoerythrin, PE) in the phycobiliprotein extract absorption spectra of the red mutant were clearly higher than those of the wild type. The PE content per area of the red mutant thallus was significantly higher than that of the wild type, and the ratios of PE to phycocyanin (PC) and PE to chlorophyll a were significantly higher in the red mutant than in the wild type. The net photosynthetic oxygen evolution rates of the red mutant and wild type cultured under different light intensities demonstrated that the oxygen evolution rates of all samples decreased with increasing light intensities, whereas the oxygen evolution rate of the red mutant was higher than that of the wild type under the same light intensities. The light response curves also indicated that the light-saturated photosynthetic rates (P max ), light saturation points (LSPs), and light compensation points (LCPs) of both strains decreased to different degrees with increasing light intensities and that the P max and LSP values of the red mutant were significantly higher than those of the wild type under identical light intensities. In contrast, there were no obvious differences in the respiration rate (R d ) and in the LCP. The F v /F m values of both strains were similar before light treatment, whereas the F v /F m of the red mutants after culture under different light intensities (120 and 200 μmol photons m −2 s −1 ) for 3 days were significantly higher than those values of the wild type. Moreover, ΔF/F m ′ also exhibited a similar variation. Based on these results, the high content of PE in the P. haitanensis red mutant causes a steady red phenotype, and the increase in the PE concentration and the decrease in the thickness of the thallus may enhance its photosynthetic physiological activity. Thus, the red mutant of P. haitanensis may provide good material for Pyropia breeding.

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Characterization of photosystem II in salt-stressed cyanobacterial Spirulina platensis cells

Cited by 55 publications

References 66 publications

The Acceptor Side of Photosystem II Is the Initial Target of Nitrite Stress in Synechocystis sp. Strain PCC 6803

The Acceptor Side of Photosystem II Is the Initial Target of Nitrite Stress in Synechocystis sp. Strain PCC 6803

Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions

Study on the photosynthetic characteristics of a Pyropia haitanensis red mutant cultured under different light intensities

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