Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro

Chhor, Vibol; Charpentier, Tifenn Le; Lebon, Sophie; Oré, Marie-Virgine; Celador, Idoia Lara; Josserand, Julien; Degos, Vincent; Jacotot, Étienne; Hagberg, Henrik; Sävman, Karin; Mallard, Carina; Gressèns, Pierre; Fleiss, Bobbi

doi:10.1016/j.bbi.2013.02.005

Cited by 546 publications

(594 citation statements)

References 88 publications

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“…The M1 phenotype has been related to release of pro-inflammatory cytokines, cytotoxicity, radical oxygen species (ROS) release, auto antigen presentation to T cells and therefore contributing with disease progression as has been observed Alzehimer's disease, MS and stroke studies (Chhor et al 2013, Rawji et al 2016). The M2 anti-inflammatory phenotype has been related with the production of anti-inflammatory cytokines and is associated with regeneration properties and wound healing Zhang 2015, Ransohoff andPerry 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Microglia/macrophage phenotypes can be recognized by changes in the expression of specific molecules. NOS-2, TNFα, COX-2, and IL-1β are characteristic molecules of a M1 phenotype while arginase-1, CD206, Ym-1 and FIZZ-1 are considered M2-phenotype markers 21 (Chhor et al 2013, Fernando et al 2014, Gensel and Zhang 2015. However, recent studies in vitro and in vivo have revealed that reactive microglia/macrophages are able to co-express M1 and M2 markers.…”

Section: Microglia/macrophage Activation Phenotypementioning

confidence: 99%

“…Recent studies have identified M2 phenotype subtypes that have been classified according to specific protein expression and possible functions. M2a has been associated with regeneration and repair, M2b is considered immunoregulatory and M2c immunosuppressive (Chhor et al 2013, Miron and Franklin 2014, Mosser and Edwards 2008, Ransohoff and Perry 2009. Microglia/macrophage phenotypes can be recognized by changes in the expression of specific molecules.…”

Section: Microglia/macrophage Activation Phenotypementioning

confidence: 99%

See 2 more Smart Citations

Mechanisms Involved in the Remyelinating Effect of Sildenafil

Díaz-Lucena

Gutièrrez‐Mecinas

Moreno

et al. 2017

J Neuroimmune Pharmacol

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ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials dʼinvestigació i docència en els termes establerts a lʼart. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix lʼautorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No sʼautoritza la seva reproducció o altres formes dʼexplotació efectuades amb finalitats de lucre ni la seva comunicació pública des dʼun lloc aliè al servei TDX. Tampoc sʼautoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices.WARNING.

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Section: Discussionmentioning

confidence: 99%

Section: Microglia/macrophage Activation Phenotypementioning

confidence: 99%

Section: Microglia/macrophage Activation Phenotypementioning

confidence: 99%

See 1 more Smart Citation

Mechanisms Involved in the Remyelinating Effect of Sildenafil

Díaz-Lucena

Gutièrrez‐Mecinas

Moreno

et al. 2017

J Neuroimmune Pharmacol

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“…There have been a number of studies that demonstrate the beneficial neuroprotective and neuroreparative effects of M2 switching [68][69][70][71]. These studies have focused on the importance of various M2 markers such as IL-4 [72,73], IL-10 [72,74,75], IL-1RA [76], TGF-β [77,78], CD206 [72,79], arginase 1 [79,80], granulocyte macrophage colony-stimulating factor [72], insulin-like growth factor 1 [81,82], and peroxisome proliferator-activated receptor-γ [70,71,83]. Even though our understanding of the role of these individual factors have become more insightful, it is very difficult to achieve beneficial neuroprotective effects by modulating just one of these factors.…”

Section: Targeting Microglia As a Therapeutic Strategymentioning

confidence: 99%

Is Immunomodulation a Principal Mechanism Underlying How Cell-Based Therapies Enhance Stroke Recovery?

Satani

Savitz

2016

Neurotherapeutics

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Inflammation within the brain and in peripheral tissues contributes to brain injury following ischemic stroke. Therapeutic modulation of the inflammatory response has been actively pursued as a novel stroke treatment approach for decades, without success. In recent years, extensive studies support the high potential for cell-based therapies to become a new treatment modality for stroke and other neurological disorders. In this review, we explore different types of cellular therapies and discuss how they modulate central and peripheral inflammatory processes after stroke. Apart from identifying potential targets for cell therapy, we also discuss paracrine and immunomodulatory mechanisms of cell therapy.

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“…As a result, they may contribute to injury progression. M2 populations on the other hand are believed to play a role in angiogenesis, remodeling of the extracellular matrix, and support regeneration following injury [103]. When appropriately queued, microglia can also release neurotrophins to augment neuronal growth and survival [104].…”

Section: Mechanisms Of Neural Injury In the Traumatic Penumbramentioning

confidence: 99%

Traumatic Penumbra: Opportunities for Neuroprotective and Neurorestorative Processes

Regner¹,

Meirelles²,

Simon³

2018

Traumatic Brain Injury - Pathobiology, Advanced Diagnostics and Acute Management

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Traumatic brain injury (TBI) is a major cause of morbidity and mortality worldwide. Understanding the pathophysiology of TBI is crucial for the development of more effective therapeutic strategies. At the moment of the traumatic impact, transfer of kinetic forces causes neurologic damage; this primary injury triggers a secondary wave of biochemical cascades, together with metabolic and cellular changes, called secondary neural injury. These areas of ongoing secondary injury, or areas of "traumatic penumbra," represent crucial targets for therapeutic interventions. This chapter is focused on the interplay between progression of parenchymal injury and the neuroprotective and neurorestorative processes that are emerging and developing subsequently to traumatic impact. Thus, we emphasized the role of traumatic penumbra in TBI pathogenesis and suggested a crucial contribution of the neurovascular units (NVUs) and paracrine effects of exosomes and miRNAs in promoting neurological recovery.

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Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro

Abstract: Highlight► A unique catalogue of phenotype markers and neuronotoxic effects of polarised primary microglia, as a comparative tool to screen neurotherapies.

Cited by 546 publications

References 88 publications

Mechanisms Involved in the Remyelinating Effect of Sildenafil

Mechanisms Involved in the Remyelinating Effect of Sildenafil

Is Immunomodulation a Principal Mechanism Underlying How Cell-Based Therapies Enhance Stroke Recovery?

Traumatic Penumbra: Opportunities for Neuroprotective and Neurorestorative Processes

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