Characterization of pHEMA-based hydrogels that exhibit light-induced bactericidal effect via release of NO

Halpenny, Genevieve M.; Steinhardt, Rachel C.; Okialda, Krystle A.; Mascharak, Pradip K.

doi:10.1007/s10856-009-3795-0

Cited by 42 publications

(41 citation statements)

References 31 publications

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“…PHEMA-based hydrogels have been used for artificial skin manufacturing and wound dressing applications [153,218] and many bioactive substances have been incorporated into these materials [153]. An interesting example is the case of a hybrid dressing consisting of a pHEMA core (containing a light-activated NO donor) and a PU coating for use on chronic wounds [246]. One major advantage of this material is the fact that NO release can be controlled by light exposure.…”

Section: Polyurethanes (Pus)mentioning

confidence: 99%

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

et al. 2013

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a b s t r a c tDiabetic foot ulcers (DFUs) are a chronic, non-healing complication of diabetes that lead to high hospital costs and, in extreme cases, to amputation. Diabetic neuropathy, peripheral vascular disease, abnormal cellular and cytokine/chemokine activity are among the main factors that hinder diabetic wound repair. DFUs represent a current and important challenge in the development of novel and efficient wound dressings. In general, an ideal wound dressing should provide a moist wound environment, offer protection from secondary infections, remove wound exudate and promote tissue regeneration. However, no existing dressing fulfills all the requirements associated with DFU treatment and the choice of the correct dressing depends on the wound type and stage, injury extension, patient condition and the tissues involved. Currently, there are different types of commercially available wound dressings that can be used for DFU treatment which differ on their application modes, materials, shape and on the methods employed for production. Dressing materials can include natural, modified and synthetic polymers, as well as their mixtures or combinations, processed in the form of films, foams, hydrocolloids and hydrogels. Moreover, wound dressings may be employed as medicated systems, through the delivery of healing enhancers and therapeutic substances (drugs, growth factors, peptides, stem cells and/or other bioactive substances). This work reviews the state of the art and the most recent advances in the development of wound dressings for DFU treatment. Special emphasis is given to systems employing new polymeric biomaterials, and to the latest and innovative therapeutic strategies and delivery approaches.

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Section: Polyurethanes (Pus)mentioning

confidence: 99%

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

et al. 2013

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“…27-32 Such NO donors have been employed to inhibit enzymes, 33 trigger apoptosis in human breast cancer cells, 34 and eradicate pathogenic bacteria 35 under the control of light. In order to explore whether the design principles could be utilized in synthesizing CORMs, we have now investigated the CO-releasing behavior of three iron carbonyls derived from three pentadentate polypyridine ligands shown in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Designed Iron Carbonyls as Carbon Monoxide (CO) Releasing Molecules: Rapid CO Release and Delivery to Myoglobin in Aqueous Buffer, and Vasorelaxation of Mouse Aorta

Gonzalez

Fry²,

Burt³

et al. 2011

Inorg. Chem.

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The physiological roles of CO in neurotransmission, vasorelaxation and cytoprotective activities have raised interest in the design and syntheses of CO-releasing materials (CORMs) that could be employed to modulate such biological pathways. Three iron-based CORMs namely, [(PaPy3)Fe(CO)](ClO4) (1), [(SBPy3)Fe(CO)](BF4)2 (2) and [(Tpmen)Fe(CO)](ClO4)2 (3), derived from designed polypyridyl ligands have been synthesized and characterized by spectroscopy and X-ray crystallography. In these three Fe(II) carbonyls, the CO is trans to a carboxamido-N (in 1), an imine-N (in 2) and a tertiary amine-N (in 3) respectively. This structural feature has been correlated to the strength of the Fe-CO bond. The CO-releasing properties of all three carbonyls have been studied in various solvents under different experimental conditions. Rapid release of CO is observed with 2 and 3 upon dissolution in both aqueous and nonaqueous media in the presence and absence of dioxygen. With 1, CO release is observed only under aerobic conditions and the final product is an oxo-bridged diiron species while with 2 and 3, the solvent bound [(L)Fe(CO)]2+ (where L = SBPy3 or Tpmen) results upon loss of CO under both aerobic and anaerobic conditions. The apparent rates of CO loss by these CORMs are comparable to other CORMs such as [Ru(glycine)(CO)3Cl] reported recently. Facile delivery of CO to reduced myoglobin has been observed with both 2 and 3. In tissue bath experiments, 2 and 3 exhibit rapid vasorelaxation of mouse aorta muscle rings. Although the relaxation effect is not inhibited by the soluble guanylate cyclase inhibitor ODQ, significant inhibition is observed with the BKCa channel blocker iberiotoxin.

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“…In addition, spatial release of NO was controlled by using a photomask to shield areas where NO-release was not desired. A poly(2-hydroxyethyl methacrylate)-based material was later employed in antibiotic studies, where the photo-released NO from 1 , in combination with singlet oxygen or hydrogen peroxide, demonstrated bactericidal effects against the Gram-negative bacteria P. aeruginosa and E. coli 20. Herein, we report the fabrication of a prototype fiber optic NO delivery system based on 1•HM and demonstrate its utility in remote-controlled, localized delivery of NO, and the results of antibiotic studies in the absence of other growth attenuators .…”

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confidence: 99%

Eradication of Pathogenic Bacteria by Remote Delivery of NO via Light Triggering of Nitrosyl-Containing Materials

Halpenny

Gandhi

Mascharak

2010

ACS Med. Chem. Lett.

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Although nitric oxide (NO) delivery systems have been fabricated with sol-gel-based materials, remote control of such systems with light has not been achieved. In this work, a fiber optic-based NO delivery system is described in which the photoactive metal-nitrosyl, [Mn(PaPy3)(NO)]ClO4 (1), has been employed in a sol-gel material. The material (1•FO) contains the manganese-nitrosyl which releases NO upon illumination with visible light. The NO-releasing capacity of 1•FO has been measured with an NO-sensitive electrode and the spatial diffusion of NO in solution has been visualized using the Griess reaction. The utility of 1•FO has been demonstrated in effective reduction of bacterial loads of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA). The results suggest that a device that releases NO via illumination by optical fiber may have clinical applications in combating infections with both Gram-positive, Gram-negative and to some degree antibiotic resistant bacteria.

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Characterization of pHEMA-based hydrogels that exhibit light-induced bactericidal effect via release of NO

Cited by 42 publications

References 31 publications

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

Designed Iron Carbonyls as Carbon Monoxide (CO) Releasing Molecules: Rapid CO Release and Delivery to Myoglobin in Aqueous Buffer, and Vasorelaxation of Mouse Aorta

Eradication of Pathogenic Bacteria by Remote Delivery of NO via Light Triggering of Nitrosyl-Containing Materials

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