Characterization of pH Dependent Growth Response of Agriculturally Important Microbes for Development of Plant Growth Promoting Bacterial Consortium

Kumar, Anil; Kumari, Menka; Swarupa, Preeti; Shireen, Shireen

doi:10.22207/jpam.13.2.43

Cited by 15 publications

(11 citation statements)

References 15 publications

(13 reference statements)

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“…9,10 These three isolates were also tolerant and grew in a broad pH range (pH 3.5-10). 10 These three isolates were used in this study.…”

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 98%

“…Three bacterial isolates (E-2, T-2, and T-1) were obtained from Kumar's laboratory at Central University of Jharkhand, India. 9,10 All three isolates exhibited four plant growth-promoting characteristics: (1) nitrogen fixation, (2) phosphate solubilization, (3) siderophore production, and (4) Indole acetic acid production. 9,10 These three isolates were also tolerant and grew in a broad pH range (pH 3.5-10).…”

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 99%

“…9,10 All three isolates exhibited four plant growth-promoting characteristics: (1) nitrogen fixation, (2) phosphate solubilization, (3) siderophore production, and (4) Indole acetic acid production. 9,10 These three isolates were also tolerant and grew in a broad pH range (pH 3.5-10). 10 These three isolates were used in this study.…”

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 99%

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Validation and Evaluation of Plant Growth Promoting Potential of Rhizobacteria Towards Paddy Plants

Kumari¹,

Swarupa²,

Kumar³

2022

J. Pure Appl. Microbiol.

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This study aimed to characterize, validate, and evaluate the plant growth potential of bacterial isolates (E-2, T-2, and T-1) to determine their suitability for application as biofertilizers and/or plant-biostimulants. The plant growth-promoting potential of bacteria (E-2, T-2, and T-1) has been validated in a hydroponic study on paddy plants by inoculating bacterial isolates and monitoring the phenotypic and plant growth responses. The applicability of bacteria was tested based on their tolerance to salinity, susceptibility to antibiotics, and identification based on 16S rDNA sequencing. The isolates E-2, T-2, and T-1 improved plant growth variably and significantly (P < 0.05 at 95% confidence interval) when inoculated into the plant growth matrix, ensuring nutrient availability to the plants grown under a nutrient (nitrate or phosphate) deprived growth matrix. Isolates E-2, T-2, and T-1 grew at salt (NaCl) concentrations of 7%, 6%, and 6%, respectively, and were tolerant to saline conditions. Although these three isolates exhibited resistance to certain antibiotics, they were susceptible to a large number of readily available antibiotics. Isolates E-2, T-2, and T-1 were identified as Klebsiella sp. strain BAB-6433, Citrobacter freundii strain R2A5, and Citrobacter sp. DY1981 respectively, and all of these may be assigned to Risk-Group-2 and hence are safe in view of their susceptibility to readily available antibiotics. Hence, these isolates are promising for extensive evaluation as bioinoculants to ecologically improve soil quality, fertility, crop growth, and yield.

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“…9,10 These three isolates were also tolerant and grew in a broad pH range (pH 3.5-10). 10 These three isolates were used in this study.…”

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 98%

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 99%

Section: Materials and Methods Source And Information Of The Microbia...mentioning

confidence: 99%

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Validation and Evaluation of Plant Growth Promoting Potential of Rhizobacteria Towards Paddy Plants

Kumari¹,

Swarupa²,

Kumar³

2022

J. Pure Appl. Microbiol.

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“…From the results it can be observed that the pH of the soil was slightly acidic which is suitable for the growth of the most of plants (Kumar et al 2019). In addition, some useful microorganisms can tolerate acidic pH and are applicable for crop enhancement (Kumar et al 2019). Soil is the largest deposit of heavy metals such as arsenic and their compounds.…”

Section: Discussionmentioning

confidence: 99%

Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

Poudel¹,

Nepal²,

Magar³

et al. 2019

TU J. Microbiol.

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Objectives: To isolate arsenic resistant Bacillus spp. and to determine plant growth promoting activities. Methods: Eighteen soil samples were collected from the agricultural soil of Terai region of Nepal. Selective isolation of Bacillus species was done by heating the soil at 80 ºC for 15 minutes before the isolation. Nutrient agar was used as an isolation medium. Screening of arsenic resistant Bacillus species was done using nutrient agar supplemented with 100 ppm sodium arsenate and sodium arsenite. For plant growth promoting activity; IAA production was detected taking 0.1% tryptophane and measuring absorbance at 540 nm, NH3 production was tested by Nessler’s reagent and phosphate solubilization activity was detected by growing colonies on Pikovskaya’s agar. Sugar assimilation test was performed to identify the isolates. Most potent arsenic resistant isolate was identified by 16S rRNA gene sequencing. Results: Among 54 randomly selected isolates, 42 were found to be Gram-positive rod-shaped, spore-forming while 12 isolates were Gram-negative bacteria. The isolates IN12a, M12a and BG34a showed growth on 100 ppm sodium arsenite containing NA. Only isolate M12a tolerated up to 1000 ppm and 15000 ppm of sodium arsenite and sodium arsenate respectively, while other isolates could not grow above 400 ppm sodium arsenite. The isolates IN12a and M12a were able to produce IAA and solubilize phosphate while BG34a could not. Both the isolates IN12a and M12a were able to utilize the sugars glucose, fructose, lactose, sucrose, galactose, mannose, mannitol, maltose and xylose. Based on the 16S rRNA gene sequencing, isolate M12a was identified to be Bacillus flexus with highest similarity of 99.2%. Conclusion: Arsenic resistant and plant growth promoting Bacillus spp. was isolated from the agricultural soil of Terai region of Nepal

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“…A high concentration of arsenic in the drinking water of the Terai region has been a long-standing public health issue of Nepal [ 40 ]. The soil profile of processed samples in this study reveals a slightly acidic pH (5-6); while soil microbes exhibit tolerance to a wide range of pH (3–13) [ 41 ]. Among 158 isolates of Bacillus species, five isolates N 4-1, RW, KR7-12, Bhw 4-1, and BW2-2 were found to be arsenic tolerant in culture media and could grow on NB containing a sodium arsenite concentration of >600 ppm.…”

Section: Discussionmentioning

confidence: 99%

Detection of Bacillus Species with Arsenic Resistance and Plant Growth Promoting Efficacy from Agricultural Soils of Nepal

et al. 2022

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Arsenic contamination in soil and water is one of the major environmental problems in multiple countries including Nepal imposing a serious threat to the ecosystem and public health. Many soil bacteria can detoxify arsenic, including genus Bacillus. With an objective to gauge the plant growth-promoting activities of arsenic-resistant Bacillus species, 36 samples (soil, rice, cauliflower, and beans) were collected from the Terai region of Nepal. For selective isolation of Bacillus species, each sample was heated at 80°C for 15 min before the inoculation into nutrient agar (NA). Following the standard protocol, arsenic-resistant Bacillus species were screened using NA supplemented with 100 ppm sodium arsenate and sodium arsenite. Among 158 randomly selected isolates, only five isolates were able to tolerate sodium arsenite concentration up to 600 ppm. Notably, all five isolates were able to produce indole acetic acid (IAA), a plant hormone, and solubilize phosphate. Based on biochemical analysis and 16S rRNA gene sequencing, isolates N4-1, RW, KR7-12, Bhw1-4, and BW2-2 were identified as B. subtilis subsp. stercosis, B. flexus, B. licheniformis, B. cereus, and B. flexus, respectively. To the best of our knowledge, this is the first study showing the presence of arsenic-resistant B. flexus in Nepalese soil with plant growth-promoting traits. Possible utilization of these Bacillus strains could facilitate the novel bioremediation pathway to reduce the toxic effect of arsenic from the soil and water in the Terai region of Nepal.

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Characterization of pH Dependent Growth Response of Agriculturally Important Microbes for Development of Plant Growth Promoting Bacterial Consortium

Cited by 15 publications

References 15 publications

Validation and Evaluation of Plant Growth Promoting Potential of Rhizobacteria Towards Paddy Plants

Validation and Evaluation of Plant Growth Promoting Potential of Rhizobacteria Towards Paddy Plants

Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

Detection of Bacillus Species with Arsenic Resistance and Plant Growth Promoting Efficacy from Agricultural Soils of Nepal

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