Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam

Huy, Nguyễn Quang; Jin, Sha; Amada, Kei; Haruki, Mitsuru; Huu, Nguyen Ba; Hang, Dinh Thuy; Ha, Dong-Soo; Imanaka, Tadayuki; Morikawa, Masaaki; Kanaya, Shigenori

doi:10.1016/s1389-1723(99)80184-4

Cited by 43 publications

(23 citation statements)

References 11 publications

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“…A colony surrounded by an emulsified halo was considered positive for BS production (Morikawa et al 2000). The diameter of the cleared zone on oil surface was visualized and measured after 30 s. Huy et al (1999) reported that this observed emulsified halo correlates with surfactant activity and is known as displacement activity.…”

Section: Oil-spreading Techniquementioning

confidence: 99%

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Nwaguma

Chikere

Okpokwasili

2016

Bioresour. Bioprocess.

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Background/aim: Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness. Hydrocarbon-polluted soils have been major sources of biosurfactant-producing bacteria; resultantly, this study had been aimed at isolating and characterizing biosurfactant produced by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria. Methodology:The biosurfactant screening techniques employed were emulsification assay, emulsification index (E 24 ), lipase activity, haemolytic assay, oil spreading, and tilted glass slide. The bacterial isolate was identified based on phenotypic, biochemical, and molecular means. Thin-layer chromatography (TLC) and gas chromatography mass spectrometry (GC-MS) analyses were used in the classification and characterization of the biosurfactant produced. The biosurfactant produced was applied on selected hydrocarbons to determine its emulsifying capacity. Results:The phylogenetic tree analysis of the 16S rRNA gene classified the isolate as K. pneumoniae strain IVN51. The sequence obtained from the isolate has been deposited in GenBank under the accession number KT254060.1. The result obtained from the study revealed high biosurfactant activity with a maximum E 24 of 60 % compared to E 24 of 70 % by sodium dodecyl sulphate (SDS). In addition, the biosurfactant showed emulsifying activity against the following hydrocarbons: petrol, kerosene, xylene, toluene, and diesel. The optimum cultural conditions (temperature, pH, carbon, nitrogen, hydrocarbon, inoculum concentration, and incubation time) for growth and biosurfactant production by K. pneumoniae IVN51 were determined. The biosurfactant was characterized as a phospholipid using TLC, while the GC-MS analysis identified the phospholipid as phosphatidylethanolamine. Conclusion:This study has demonstrated the capacity of K. pneumoniae strain IVN51 isolated from hydrocarbonpolluted soil to produce biosurfactant and the effectiveness of the produced biosurfactant in emulsifying different hydrocarbons. Furthermore, the biosurfactant produced was found to belong to the class, phospholipids based on the TLC and GC-MS analyses.

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Section: Oil-spreading Techniquementioning

confidence: 99%

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Nwaguma

Chikere

Okpokwasili

2016

Bioresour. Bioprocess.

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“…Plaza et al (2004) and Youssef et al (2006) demonstrated that the oil spreading technique is a reliable method to detect biosurfactant production by diverse microorganisms. The assay was also applied for screening by Huy et al (1999). Figure 5 lists the diameters of the clear zones on the oil surface obtained from oil displacement testing with the crude biosurfactant produced from different carbon and nitrogen sources by P. aeruginosa TMN.…”

Section: Oil Displacement Testmentioning

confidence: 99%

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

106

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-Pseudomonas aeruginosa TMN was used to produce rhamnolipid (RL) from a variety of carbon and nitrogen substrates. The most favorable carbon sources for RL production were glucose and glycerol (both at 40 g/L), giving a RL yield of 0.3 and 0.25 g/L, respectively. Meanwhile, sodium nitrate appeared to be the preferable nitrogen source, resulting in a RL production of 0.34g/L. Rhamnolipid production from P. aeruginosa TMN was affected by temperature, pH and agitation rate, with 37 °C, pH 7 and 200 rpm agitation favorable for rhamnolipid production. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) and electro spray ionization-mass spectrometry (ESI-MS) analyses indicated that the purified product contained one type of commonly found rhamnolipid, which is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate. The rhamnolipid product can reduce the surface tension of water to 34 mN/m with a critical micelle concentration of nearly 18.75 mg/L and emulsified kerosene by 46%. P. aeruginosa TMN strain is a potential source of rhamnolipid biosurfactant, which could be used for the development of bioremediation processes in the marine environment.

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“…Since many naturally occurring micr oor ganisms have th e ability to utilize hydrocarbons as the sole source of carbon and are widely distributed (Kor en et al, 2003), the biodegradation of these compounds is common in nature. Bacterial degradation of petroleum has been known for over 50 years, responsible bacteria have mostly been isolated from surface areas, such as soils, petroleum storage tanks and oil spills (Huy et al, 1999). There are numerous reports of isolation of petroleum hydrocarbon degrader bacteria from oil exposed areas (Kasai et al, 2001;Tazaki, 2005).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel

Kebria

Ahmad

Ganjidoust

et al. 2009

Int. J. Environ. Sci. Technol.

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ABSTRACT:The ability of native bacteria to utilize diesel fuel as the sole carbon and energy source was investigated in this research. Ten bacterial strains were isolated from the oil refinery field in Tehran, Iran. Two biodegradation experiments were performed in low and high (500 and 10000 ppm, respectively) concentration of diesel fuel for 15 days. Only two isolates were able to efficiently degrade the petroleum hydrocarbons in the first test and degraded 86.67 % and, 80.60 % of diesel fuel, respectively. The secondary experiment was performed to investigate the toxicity effect of diesel fuel at high concentration (10000 ppm). Only one strain was capable to degrade 85.20 % of diesel fuel at the same time (15 days). Phenotype and phylogeny analysis of this strain was characterized and identified as dieseldegrading bacteria, based on gram staining, biochemical tests, 16S rRNA gene sequence analysis. These results indicate that this new strain was Bacillus sp. and could be considered as Bacillus Cereus with 98 % 16 S rRNA gene sequence similarity. The results indicate that native strains have great potential for in situ remediation of diesel-contaminated soils in oil refinery sites.

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Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam

Cited by 43 publications

References 11 publications

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel

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