Characterization of persistent follicles induced by prolonged treatment with progesterone in dairy cows: An experimental model for the study of ovarian follicular cysts

Díaz, Pablo Uriel; Stangaferro, M.L.; Gareis, Natalia Carolina; Silvia, W.J.; Matiller, Valentina; Salvetti, Natalia Raquel; Rey, Florencia; Barberis, F.; Cattáneo, L.; Ortega, Hugo Héctor

doi:10.1016/j.theriogenology.2015.06.015

Cited by 46 publications

(46 citation statements)

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“…131/12) The model used here has been previously optimized [Díaz et al, 2015] and is summarized in Figure 1 . This model was performed in nonlactating Holstein cows ( n = 25) with regular estrous cycles.…”

Section: Induction Of Follicular Persistence By Low Doses Of Progestementioning

confidence: 99%

“…This model was performed in nonlactating Holstein cows ( n = 25) with regular estrous cycles. Ovarian activity was synchronized starting with the protocol G6G [Pursley et al, 1995;Bello et al, 2006] with modifications [Díaz et al, 2015]. Once synchronized, the cows were divided into 5 groups: the control group ( n = 5), and groups P0 (treated with progesterone up to the expected day of ovulation; n = 5), P5 (5 days of follicular persistence since the expected day of ovulation; n = 5), P10 (10 days of follicular persistence since the expected day of ovulation; n = 5), and P15 (15 days of follicular persistence since the expected day of ovulation; n = 5).…”

Section: Induction Of Follicular Persistence By Low Doses Of Progestementioning

confidence: 99%

“…anti-Müllerian hormone BMP bone morphogenetic protein COD cystic ovarian disease FSH follicle-stimulating hormone IGF LH insulin-like growth factor luteinizing hormone intravaginal progesterone device (750 mg of micronized progesterone; Pro-Ciclar P4-Zoovet ® , Santa Fe, Argentina) to obtain the subluteal concentrations of progesterone (1-2 ng/mL), as described in Díaz et al [2015]. The device was kept in the cows until the expected day of ovulation in group P0, for 5 days after the expected day of ovulation in group P5, and for 8 days in groups P10 and P15.…”

Section: Amhmentioning

confidence: 99%

“…In group P15, a third intravaginal progesterone-releasing device was inserted on day 11 of persistence, 1 day before the removal of the second one ( Fig. 1 ) [for details see Díaz et al, 2015].…”

Section: Amhmentioning

confidence: 99%

“…In control cows, bilateral ovariectomy was performed 2 days after completion of the synchronization protocol, whereas in groups P0, P5, P10, and P15, ovariectomy was performed on day 0 (the expected day of ovulation) and on days 5, 10, and 15 of follicular persistence, respectively [Díaz et al, 2015] ( Fig. 1 ).…”

Section: Amhmentioning

confidence: 99%

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Changes in the Proliferation/Apoptosis Balance in the Bovine Ovary: A Key Early Event in Follicular Persistence

Belotti

Stassi

Velázquez

et al. 2017

Cells Tissues Organs

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The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone to detect incipient changes during cystic ovarian disease development on the expected day of ovulation (day 0) and after 5, 10, and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX, and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in the P0 group (treated with progesterone up to the expected day of ovulation) and in the control group. A decrease in cell proliferation was detected after 5 days of persistence (P5) in relation to P0 (p < 0.05). Similar changes were found in the granulosa cells of the persistent follicles in relation to the control group (p < 0.05). Caspase-3 expression was similar in granulosa cells of antral follicles at early stages of persistence, with an increase after 15 days of persistence (p < 0.05). In the granulosa cells of group P10 (10 days of persistence), caspase-3 expression was reduced relative to that of antral follicles from the control group (p < 0.05). BCL2 expression was higher in granulosa cells of the persistent follicles of group P0 relative to the control follicles, with no changes in BAX expression, which was increased in persistent follicles of group P15 (p < 0.05). Similar results were observed in theca cells at initial stages of persistence. The results show that, initially, proliferation is maintained with low apoptosis and an increase in cell survival.

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Section: Induction Of Follicular Persistence By Low Doses Of Progestementioning

confidence: 99%