Characterization of Pectobacterium species from Iran using biochemical and molecular methods

Ravari, Sareh Baghaee; Rahimian, H.; Shams-Bakhsh, Masoud; López‐Solanilla, Emilia; Antúnez-Lamas, María; Rodrı́guez-Palenzuela, Pablo

doi:10.1007/s10658-010-9704-z

Cited by 60 publications

(43 citation statements)

References 26 publications

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“…Thus, it appears that there was no correlation between the presence or absence of a T3SS in these strains and the ability to cause disease. These observations are consistent with those of other researchers (Ma et al 2007;Kim et al, 2009;Pitman et al 2010;Baghaee-ravari et al 2011). …”

Section: Discussionsupporting

confidence: 94%

“…However, there have been several reports of its occurrence in potatoes (Ma et al, 2007;Pitman et al, 2008;Kim et al, 2009;Baghaee-Ravari et al, 2011). Interestingly, Ma et al (2007) previously identified several P. wasabiae strains lacking a type III secretion system (T3SS).…”

Section: Discussionmentioning

confidence: 99%

“…This is the first report of P. wasabiae in potatoes in South Africa. Previous reports of P. wasabiae strains infecting potatoes were in the USA, New Zealand and Iran (Kim et al, 2009;Pitman et al, 2008;Baghaee-Ravari et al, 2011).…”

Section: Phylogenetic Analysis Of Atypical South African Strainsmentioning

confidence: 99%

“…carotovorum and brasiliensis causing blackleg, tuber soft rot, stem wilt and stem rot symptoms (Kim et al, 2009;Pitman et al, 2010;van der Merwe et al, 2010;Baghaee-Ravari et al, 2011). Because there is an overlap in symptoms caused by the SRE, it is nearly impossible to identify the causal agent by looking at symptoms.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

et al. 2012

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Pectolytic bacteria were isolated from potato tubers and stems showing tuber soft rot and blackleg symptoms. Approximately half (52%) of the isolates could grow at both 27 and 37 °C while another half (48%) failed to grow at 37 °C. All isolates could be amplified with primers specific to the pectate lyase (pel) gene. Carbon utilisation profiles could not conclusively identify these isolates. PCR amplification using primers specific for Pectobacterium carotovorum subsp. brasiliensis was positive for all isolates that grew at 37°C. However, the group that did not grow at 37 °C failed to amplify with P. atrosepticum specific primers. To characterise this group of isolates, the intergenic transcribed spacer region (ITS) was amplified and PCR products digested with two restriction enzymes (RsaI and CfoI) to generate ITS-PCR-RFLP profiles. The profiles of these new isolates were compared to those of the type strains of other pectolytic bacteria. Profiles of five of the selected atypical strains generated with the enzyme CfoI appeared to be most similar to those of P. wasabiae type strain. Phylogenetic analysis using concatenated partial gene sequences of housekeeping genes mdh and gapA clustered these isolates together with those of P.wasabiae reference strains thus confirming their identity. These strains were virulent on potato tubers and stems but did not elicit hypersensitive response on tobacco plants. This is the first report of P. wasabiae causing soft rot and blackleg of potatoes in South Africa.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Atypical South African Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

et al. 2012

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“…Previously, P. c. ssp. carotovorum strains had been shown to vary in their ability to cause HR in tobacco and the presence of type III secretion system (Yap et al, 2004;Kim et al, 2009;Baghaee-Ravari et al, 2011;Nabhan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Pectobacterium carotovorum subsp. carotovorum - the causal agent of broccoli soft rot in Serbia

Gašić

Gavrilović

Dolovac

et al. 2014

Pesticidi i fitomedicina

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Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37°C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor "fried egg" colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.

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Screening bactericidal effect of Pectobacterium carotovorum subsp. carotovorum strains against causal agent of potato soft rot

Kazemi-Zaromi

Ravari

Khodaygan

et al. 2015

J. Basic Microbiol.

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This study focuses on the potential of Pectobacterium carotovorum subsp. carotovorum (Pcc) strains producing bacteriocin as a tool to control potato soft rot disease. Thirty out of 48 purified bacterial strains were characterized as Pcc using specific PCR and phenotypic tests. The pathogenicity and pectate degrading assays were recorded positive for 13 strains. Bacteriocin typing clustered producers into three groups according to their antimicrobial spectra. Majority of the producers except strains of group II showed antibacterial activity toward relative genus and the role of UV or mitomycin C was inductive. In addition, none of the distant genus was sensitive to Pcc bacteriocins except Rhizobium vitis. Molecular detection of four bacteriocins including carotovoricin, carosin S1, S2 and carosin D was performed. Overall, 54.5% of group I, 47.3 and 70% of groups II and III strains carried carotovoricin and four strains harbored gene corresponding to carosin S1. According to our data divers antimicrobial patterns obtained by Pcc strains and existence of new bateriocines could be possible. Moreover, our findings recommended that direct application of P29 or expression of corresponding genes of Pog22 or P21 in a nonpathogenic strain as a biocontrol agent may improve soft rot disease control.

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Characterization of Pectobacterium species from Iran using biochemical and molecular methods

Cited by 60 publications

References 26 publications

Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

Pectobacterium carotovorum subsp. carotovorum - the causal agent of broccoli soft rot in Serbia

Screening bactericidal effect of Pectobacterium carotovorum subsp. carotovorum strains against causal agent of potato soft rot

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