Characterization of Pectobacterium carotovorum subsp. carotovorum and brasiliense from diseased potatoes in Kenya

Onkendi, Edward Makori; Moleleki, Lucy N.

doi:10.1007/s10658-014-0411-z

Cited by 37 publications

(27 citation statements)

References 37 publications

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“…Two strains that are considered to be type strains of two Pectobacterium species, PC1 (P. aroidearum) and Pcb1692 (P. carotovorum ssp. brasiliense), were used in this study (Nabhan et al, 2013;Onkendi and Moleleki, 2014). Pathogens belonging to these species are able to infect monocot and dicot hosts of agricultural importance, especially under warm conditions (Nabhan et al, 2012(Nabhan et al, , 2013Onkendi and Moleleki, 2014).…”

Section: Discussionmentioning

confidence: 99%

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Joshi

Burdman

Lipsky

et al. 2015

Molecular Plant Pathology

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Several studies have reported effects of the plant phenolic acids cinnamic acid (CA) and salicylic acid (SA) on the virulence of soft rot enterobacteria. However, the mechanisms involved in these processes are not yet fully understood. Here, we investigated whether CA and SA interfere with the quorum sensing (QS) system of two Pectobacterium species, P. aroidearum and P. carotovorum ssp. brasiliense, which are known to produce N-acyl-homoserine lactone (AHL) QS signals. Our results clearly indicate that both phenolic compounds affect the QS machinery of the two species, consequently altering the expression of bacterial virulence factors. Although, in control treatments, the expression of QS-related genes increased over time, the exposure of bacteria to non-lethal concentrations of CA or SA inhibited the expression of QS genes, including expI, expR, PC1_1442 (luxR transcriptional regulator) and luxS (a component of the AI-2 system). Other virulence genes known to be regulated by the QS system, such as pecS, pel, peh and yheO, were also down-regulated relative to the control. In agreement with the low levels of expression of expI and expR, CA and SA also reduced the level of the AHL signal. The effects of CA and SA on AHL signalling were confirmed in compensation assays, in which exogenous application of N-(β-ketocaproyl)-l-homoserine lactone (eAHL) led to the recovery of the reduction in virulence caused by the two phenolic acids. Collectively, the results of gene expression studies, bioluminescence assays, virulence assays and compensation assays with eAHL clearly support a mechanism by which CA and SA interfere with Pectobacterium virulence via the QS machinery.

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Section: Discussionmentioning

confidence: 99%

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Joshi

Burdman

Lipsky

et al. 2015

Molecular Plant Pathology

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“…brasiliense was first isolated from blackleg‐diseased potato plants in Brazil (Duarte et al ., ). Since then, it has been found in association with plants with symptoms in many parts of the world (Pitman et al ., ; Van der Merwe et al ., ; De Boer et al ., ; Onkendi & Moleleki, ). In Europe, P. carotovorum subsp.…”

Section: Introductionmentioning

confidence: 98%

Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands

Wolf¹,

Haan²,

Kastelein³

et al. 2016

Plant Pathology

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In western Europe, Pectobacterium carotovorum subsp. brasiliense is emerging as a causal agent of blackleg disease. In field experiments in the Netherlands, the virulence of this pathogen was compared with strains of other Dickeya and Pectobacterium species. In 2013 and 2014, seed potato tubers were vacuum infiltrated with high densities of bacteria (10 6 CFU mL À1 ) and planted in clay soil. Inoculation with P. carotovorum subsp. brasiliense and P. atrosepticum resulted in high disease incidences (75-95%), inoculation with D. solani and P. wasabiae led to incidences between 5% and 25%, but no significant disease development was observed in treatments with P. carotovorum subsp. carotovorum, D. dianthicola or the water control. Co-inoculations of seed potatoes with P. carotovorum subsp. brasiliense and D. solani gave a similar disease incidence to inoculation with only P. carotovorum subsp. brasiliense. However, coinoculation of P. carotovorum subsp. brasiliense with P. wasabiae resulted in a decrease in disease incidence compared to inoculation with only P. carotovorum subsp. brasiliense. In 2015, seed potatoes were inoculated with increasing densities of P. carotovorum subsp. brasiliense, D. solani or P. atrosepticum (10 3 -10 6 CFU mL À1 ). After vacuum infiltration, even a low inoculum density resulted in high disease incidence. However, immersion without vacuum caused disease only at high bacterial densities. Specific TaqMan assays were evaluated and developed for detection of P. carotovorum subsp. brasiliense, P. wasabiae and P. atrosepticum and confirmed the presence of these pathogens in progeny tubers of plants derived from vacuum-infiltrated seed tubers.

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“…Of these, Pectobacterium carotovorum subsp. brasiliense is an emerging SRE with global distribution (35-40). Previous studies have shown that Pcb strain PBR1692 (referred to hence forth as Pcb 1692) is able to inhibit growth of P. carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Nkomo

et al. 2018

Preprint

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17Plant microbial communities' complexity provide a rich model for investigation on biochemical and 18 regulatory strategies involved in interbacterial competition. Within these niches, the soft rot 19 Enterobacteriaceae (SRE) comprise an emerging group of plant-pathogens inflicting soft rot/black-20 leg diseases and causing economic losses worldwide in a variety of crops. In this report, a range of 21 molecular and computational techniques are utilized to survey the contribution of antimicrobial 22Pcb1692 inhibits growth of other SRE and several potato endophytes using either the type VI 51 secretion, carbapenem or bacteriocins. Carbapenem plays a role in both inter and intrabacterial 52 competition in vitro, while the Pcb1692T6SS plays a role in interbacterial competition in planta (in 53 potato tubers). We also demonstrate that carbapenem regulation requires the presence of 54 environmental iron and oxygen in a complex network consisting of Pcb1692 Fur, SlyA, and ExpI. 55

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Characterization of Pectobacterium carotovorum subsp. carotovorum and brasiliense from diseased potatoes in Kenya

Cited by 37 publications

References 37 publications

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

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