Characterization of pectinolytic enzymes of Clostridium thermosulfurogenes

Pectinolytic activity ofClostridium thermocellum: Its use for anaerobic fermentation of sugar beet pulp

“…This strain is thus able to hydrolyse simultaneously the major hydrocarbons of parietal polymers of sugar beet pulp. Pectinolytic activity of several mesophilic anaerobic bacteria such as Clostridium felsineum (Avrova et al 1981), Clostridium multifermentans (Sheiman et al 1976), Clostridium butyricum (Obi 1981;Szymanski 1981) and a few thermophilic anaerobic bacteria such as Clostridium thermosulfurogenes (Schink and Zeikus 1983) and other strains of Clostridium and Erwinia (Schink and Zeikus 1980) have been studied. Our results showed that galacturonase and pectin lyase are both produced by Clostridium thermocellum.…”

Analysis of Pectin Methyl Esterases

“…Like plant PMEs, fungus PME can be glycosylated, and in one case a bacterial PME also appeared to be glycosylated (Van Rijssel et al 1993). In some cases, PME activity is expressed by multi-enzymic complexes also exhibiting pectate lyase (PL) (Sheiman et al 1976) or polygalacturonase (PG) (Van Rijssel et al 1993;Schink and Zeikus 1983) activities. (Table 3).…”

Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum

“…Numerous clostridial species are capable of fermenting galacturonate and gluconate, but little is known about the pathways and the associated genes contributing to this process in solventogenic clostridia [14][15][16][17][18]. An early study comparing C. acetobutylicum fermentations on substrates with varying degrees of oxidation showed fermentation of gluconate resulted in higher acetate:butyrate ratios when compared to glucose fermentations [10].…”