Characterization of Patterned Self-Assembled Monolayers and Protein Arrays Generated by the Ink-Jet Method

Pardo, Laura; Wilson, Wesley V.; Boland, Thomas

doi:10.1021/la026171u

Cited by 141 publications

(120 citation statements)

References 33 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…In addition, the small orifices are susceptible to blockage by dust particles or aggregates of material [9]. This method can be cost-effective and readily accessible to most laboratories by using modified, commercially available ink-jet printers [57].…”

Section: Microarray Formationmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

View full text Add to dashboard Cite

The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the literature in this area and conclude that for polymers, this process is best achieved in a microarray format, which enable thousands of cell-material interactions to be monitored on a single chip. Polymer microarrays can be formed by printing pre-synthesised polymers or by printing monomers onto the chip where on-slide polymerisation is initiated.The surface properties of the material can be analysed and correlated to the biological performance using high throughput surface analysis, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), Xray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. This approach enables the surface properties responsible for the success of a material to be understood, which in turn provides the foundations of future material design. The high throughput discovery of materials using polymer microarrays has been explored for many cell-based applications including the isolation of specific 2 cells from heterogeneous populations, the attachment and differentiation of stem cells and the controlled transfection of cells.Further development of polymerisation techniques and high throughput biological assays amenable to the polymer microarray format will broaden the combinatorial space and biological phenomenon that polymer microarrays can explore, and increase their efficacy. This will, in turn, result in the discovery of optimised polymeric materials for many biomaterial applications.

show abstract

Section: Microarray Formationmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

View full text Add to dashboard Cite

show abstract

“…34 Defined droplets can be dispensed with a computer-controlled pump 34 or through inkjet printing. 35,36 For all methods the biomolecule diffuses away from its source into the gel forming a concentration gradient over the cells that evolves in both space and time (Fig. 1c).…”

Section: Biological Hydrogelsmentioning

confidence: 99%

Biomolecular gradients in cell culture systems

2008

View full text Add to dashboard Cite

Biomolecule gradients have been shown to play roles in a wide range of biological processes including development, inflammation, wound healing, and cancer metastasis. Elucidation of these phenomena requires the ability to expose cells to biomolecule gradients that are quantifiable, controllable, and mimic those that are present in vivo. Here we review the major biological phenomena in which biomolecule gradients are employed, traditional in vitro gradient-generating methods developed over the past 50 years, and new microfluidic devices for generating gradients. Microfluidic gradient generators offer greater levels of precision, quantitation, and spatiotemporal gradient control than traditional methods, and may greatly enhance our understanding of many biological phenomena. For each method, we outline the salient features, capabilities, and applications.

show abstract

“…Micro contact printing with the use of polydimethylsiloxane (PDMS) stamps [1][2][3], ink-jet printing with inkjet printers [4,5], and micro-fluid techniques [6,7] have been widely used to control cell attachment and growth on a substrate by patterning ECM proteins. Recently another technique based on patterned etching of ECM layers by atmospheric-pressure plasma (APP) has been proposed [8], which the authors believe offers one of the most cost effective means for ECM patterning.…”

Section: Arrangement Of Pc12 Cells On a Silicon Chip Via Extracellulamentioning

confidence: 99%

“…The extracellular matrix (ECM) is key proteins for cell attachment and proliferation. For the arrangement of living cells on a semiconductor substrate, therefore, the formation of patterned ECM layers on the semiconductor surface is an important first step.Micro contact printing with the use of polydimethylsiloxane (PDMS) stamps [1-3], ink-jet printing with inkjet printers [4,5], and micro-fluid techniques [6,7] have been widely used to control cell attachment and growth on a substrate by patterning ECM proteins. Recently another technique based on patterned etching of ECM layers by atmospheric-pressure plasma (APP) has been proposed [8], which the authors believe offers one of the most cost effective means for ECM patterning.…”

mentioning

confidence: 99%

Arrangement of PC12 Cells on a Silicon Chip via Extracellular Matrix (ECM) Layer Patterning by Atmospheric Pressure Plasmas

Ando

Uno

Asano

et al. 2011

Plasma and Fusion Research

View full text Add to dashboard Cite

It is shown that neuronal model cells PC12 (rat adrenal pheochromocytoma cell line) can be cultured on a silicon (Si) substrate with the use of extracellular matrix (ECM) patterned by atmospheric-pressure plasmas (APPs). Arrangement of neuron cells in a desired pattern on a Si chip is an important step for the development of neuron cell chips. In the experiments, 100-200 µm wide strips of ECM (Poly-L-Lysine) layers arranged in parallel were formed on a 1 cm 2 area of a Si surface by the APP etching technique and PC12 cells were shown to grow on the ECM strips. The APP etching technique for ECM layers provides a simple mean of arranging neuron cells on a relatively large area of a Si surface. Control of cell adhesion to and proliferation on a specific surface connected with electrical circuits is a technology required for the development of next-generation biosensors/biochips that house living cells in them. Such sensors and chips, which may be called cell sensors or cell chips, are expected to serve to various applications in biology and medicine. The extracellular matrix (ECM) is key proteins for cell attachment and proliferation. For the arrangement of living cells on a semiconductor substrate, therefore, the formation of patterned ECM layers on the semiconductor surface is an important first step.Micro contact printing with the use of polydimethylsiloxane (PDMS) stamps [1-3], ink-jet printing with inkjet printers [4,5], and micro-fluid techniques [6,7] have been widely used to control cell attachment and growth on a substrate by patterning ECM proteins. Recently another technique based on patterned etching of ECM layers by atmospheric-pressure plasma (APP) has been proposed [8], which the authors believe offers one of the most cost effective means for ECM patterning.The goal of this study is to demonstrate that the APP etching method for ECM layers proposed by Ref. [8] can be also applied to the arrangement of neuron cells on a silicon (Si) chip. Specific cells proliferate only on specific ECMs, so the demonstration of HEK293 cells (Human Embryo Kidney cells) on fibronectins (which are used

show abstract

Characterization of Patterned Self-Assembled Monolayers and Protein Arrays Generated by the Ink-Jet Method

Cited by 141 publications

References 33 publications

High throughput methods applied in biomaterial development and discovery

High throughput methods applied in biomaterial development and discovery

Biomolecular gradients in cell culture systems

Arrangement of PC12 Cells on a Silicon Chip via Extracellular Matrix (ECM) Layer Patterning by Atmospheric Pressure Plasmas

Contact Info

Product

Resources

About