Characterization of Pasteurella multocida strains isolated from geese

Fowl cholera caused by Pasteurella multocida has always been a disease of global importance for poultry production. The aim of this study was to obtain more information about the epidemiology of avian P. multocida infection in southwest China and the genetic characteristics of clinical isolates. P. multocida isolates were characterized by biochemical and molecular-biological methods. The distributions of the capsular serogroups, the phenotypic antimicrobial resistance profiles, lipopolysaccharide (LPS) genotyping and the presence of 19 virulence genes were investigated in 45 isolates of P. multocida that were associated with clinical disease in poultry. The genetic diversity of P. multocida strains was performed by 16S rRNA and rpoB gene sequence analysis as well as multilocus sequence typing (MLST). The results showed that most (80.0%) of the P. multocida isolates in this study represented special P. multocida subspecies, and 71.1% of the isolates showed multiple-drug resistance. 45 isolates belonged to capsular types: A (100%) and two LPS genotypes: L1 (95.6%) and L3 (4.4%). MLST revealed two new alleles (pmi77 and gdh57) and one new sequence type (ST342). ST129 types dominated in 45 P. multocida isolates. Isolates belonging to ST129 were with the genes ompH+plpB+ptfA+tonB, whereas ST342 included isolates with fur+hgbA+tonB genes. Population genetic analysis and the MLST results revealed that at least one new ST genotype was present in the avian P. multocida in China. These findings provide novel insights into the epidemiological characteristics of avian P. multocida isolates in southwest China.

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“…Varga et al . [ 40 ] thought that discrimination between P. multocida subsp. multocida and P. multocida subsp.…”

Section: Discussionmentioning

confidence: 99%

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Cheng

Lan

et al. 2018

The Journal of Veterinary Medical Science

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“…Pasteurella multocida is the causative agent of fowl cholera and causes significant economic losses in the poultry industry (Christensen & Bisgaard, ; Harper, Boyce, & Adler, ; Varga et al., ). P. multocida can be classified into five capsular serogroups A, B, D, E, and F by serological methods and 16 serotypes by analysis of heat stable precipitin antigens (Carter, ; Christensen & Bisgaard, ; Heddleston, Gallagher, & Rebers, ).…”

Section: Introductionmentioning

confidence: 99%

Comparative pharmacokinetics of florfenicol in healthy and Pasteurella multocida‐infected Gaoyou ducks

Lan

Xiao

Jiang

et al. 2019

Vet Pharm & Therapeutics

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Pasteurella multocida is the causative agent of fowl cholera, and florfenicol (FF) has potent antibacterial activity against P. multocida and is widely used in the poultry industry. In this study, we established a P. multocida infection model in ducks and studied the pharmacokinetics of FF in serum and lung tissues after oral administration of 30 mg/kg bodyweight. The maximum concentrations reached (Cmax) were lower in infected ducks (13.88 ± 2.70 μg/ml) vs. healthy control animals (17.86 ± 1.57 μg/ml). In contrast, the mean residence time (MRT: 2.35 ± 0.13 vs. 2.27 ± 0.18 hr) and elimination half‐life (T½β: 1.63 ± 0.08 vs. 1.57 ± 0.12 hr) were similar for healthy and diseased animals, respectively. As a result, the area under the concentration curve for 0–12 hr (AUC0–12 hr) for FF in healthy ducks was significantly greater than that in infected ducks (49.47 ± 5.31 vs. 34.52 ± 8.29 μg hr/ml). The pharmacokinetic differences of FF in lung tissues between the two groups correlated with the serum pharmacokinetic differences. The Cmax and AUC0–12 hr values of lung tissue in healthy ducks were higher than those in diseased ducks. The concentration of FF in lung tissues was approximately 1.2‐fold higher than that in serum both in infected and healthy ducks indicating that FF is effective in treating respiratory tract infections in ducks.

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“…The RpoB gene analysis showed a similarity (99.38%) between the strain S13 and 964 isolated from goose host and originated from Hungary [23]. The strain S14 was related to the Chinese strain PM-L1706 (99.33%) isolated from a chicken host (MG813902).…”

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 86%

Biological proprieties of Pasteurella multocida isolate from sheep in Morocco

Boumart¹,

Bamouh²,

Jazouli³

et al. 2019

Preprint

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Background In Morocco, observations of pasteurellosis clinical cases reported by field veterinarian and lesions observed in abattoirs, suggest circulation of serotypes of pasteurellosis among small ruminants population. However, little is known on the prevalence of the main serotypes in this region and their pathogenicity. Results We investigated five suspected Pasteurella clinical cases from different provinces of Morocco. We succeeded to isolate 2 strains (S14 and S13) of Pasteurella Multocida A among the five isolates. Identification was achieved by biochemical and molecular biology methods. Phylogeny based on two genes sequence analysis (RNA16S and rpoB) suggested that the two isolates present similarity with others from different species. A pathogenicity study was conducted in mice, guinea pigs and sheep to set up a model for vaccine testing. The strain S14 was more virulent than S13 in laboratory animals and induced severe illness in sheep. Conclusion The high mortality of infected mice suggest that this animal may represent a good alternative for testing pathogenicity and vaccine efficacy.

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Characterization of Pasteurella multocida strains isolated from geese

Cited by 12 publications

References 33 publications

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Comparative pharmacokinetics of florfenicol in healthy and Pasteurella multocida‐infected Gaoyou ducks

Biological proprieties of Pasteurella multocida isolate from sheep in Morocco

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