Characterization of Pasteurella multocida isolated from ducks in China from 2017 to 2019

Xiao, Junfang; Li, Yajuan; Hu, Zezhong; Zhang, Yukun; Chang, Yung-Fu; Zhou, Qingfeng; Zhang, Yan; Zhang, Xinheng; Chen, Liyi; Li, Wenxue; Xie, Zili; Xie, Qingmei

doi:10.1016/j.micpath.2021.105196

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…P. multocida is a typical commensal of the upper respiratory tract (1,2) and can cause various animal diseases such as swine atrophic rhinitis and swine pneumonia, and hemorrhagic septicemia in various domestic poultry (3). The disease of ducks caused by P. multocida is called duck cholera or duck hemorrhagic septicemia, an acute, highly transmissible, and septicaemic infection with an incidence of 30-70% and a mortality rate of 30%-80% (4,5).…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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Duck cholera (duck hemorrhagic septicemia) is a highly contagious disease caused by Pasteurella multocida, and is one of the major bacterial diseases currently affecting the duck industry. Type A is the predominant pathogenic serotype. In this study, the genes encoding the lipoproteins VacJ, PlpE, and the outer membrane protein OmpH of P. multocida strain PMWSG-4 were cloned and expressed as proteins in E. coli. The recombinant VacJ (84.4 kDa), PlpE (94.8 kDa), and OmpH (96.7 kDa) proteins were purified, and subunit vaccines were formulated with a single water-in-oil adjuvant, while killed vaccines were prepared using a single oil-coated adjuvant. Antibody responses in ducks vaccinated with recombinant VacJ, PlpE, and OmpH proteins formulated with adjuvants were significantly antigenic (p<0.005). Protectivity of the vaccines was evaluated via the intraperitoneal challenge of ducks with 20 LD50 doses of P. multocida A: 1. The vaccine formulation consisting of rVacJ, rPlpE, rOmpH, and adjuvant provided 33.3%, 83.33%, and 83.33% protection, respectively, the vaccine formulation consisting of three recombinant proteins, rVacJ, rPlpE, rOmpH and adjuvant, was 100% protective, and the killed vaccine was 50% protective. In addition, it was shown through histopathological examination and tissue bacterial load detection that all vaccines could reduce tissue damage and bacterial colonization to varying (p<0.001). These findings indicated that recombinant PlpE or OmpH fusion proteins formulated with oil adjuvants have the potential to be used as vaccine candidates against duck cholera subunits.

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Section: Introductionmentioning

confidence: 99%

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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“…However, this bacterium can cause damage to multiple organs in addition to the respiratory organs, including the hearts, spleens, livers, kidneys, guts, and even brains, of both humans and animals after infections via respiratory tracts ( 19 – 21 ). In addition, it is common in clinical cases to recover P. multocida from organs in addition to respiratory organs ( 22 – 24 ). Therefore, we hypothesize that P. multocida invades and crosses the host respiratory epithelial barrier during infection.…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

et al. 2023

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“…The global epidemiology of FC is complex with diverse serovars mainly Heddleston serotypes 1, 3, 4 in different regions worldwide [ 4 , 43 – 45 ], which poses a substantial challenge for current commercial FC vaccines especially inactivated bacterins that were designed based on single serotype [ 14 ]. Live attenuated vaccines have been proven to confer a certain level of cross protection [ 17 ], therefore cross-immunity potential of the PMZ2 needs to be evaluated in further studies.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Zhao

Yang

Shen

et al. 2022

Vet Res

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Pasteurella multocida infection frequently causes fowl cholera outbreaks, leading to huge economic losses to the poultry industry worldwide. This study developed a novel live attenuated P. multocida vaccine strain for ducks named PMZ2 with deletion of the gatA gene and first four bases of the hptE gene, both of which are required for the synthesis of the lipopolysaccharide (LPS) outer core. PMZ2 produced a truncated LPS phenotype and was highly attenuated in ducks with a > 105-fold higher LD50 than the wild-type strain. PMZ2 colonized the blood and organs, including the spleen, liver and lung, at remarkably reduced levels, and its high dose of oral infection did not cause adverse effects on body temperatures and body weights in ducks. To evaluate the vaccine efficacy of the mutant, ducklings were inoculated orally or intranasally with PMZ2 or PBS twice and subsequently subjected to a lethal challenge. Compared with the PBS control, PMZ2 immunization stimulated significantly elevated serum IgG, bile IgA and tracheal IgA responses, especially after the boost immunization in both the oral and intranasal groups, and the induced serum had significant bactericidal effects against the wild-type strain. Furthermore, the two PMZ2 immunization groups exhibited alleviated tissue lesions and significantly decreased bacterial loads in the blood and organs compared with the PBS group post-challenge. All the ducks in the PMZ2 oral and intranasal groups survived the challenge, while 70% of ducks in the PBS group succumbed to the challenge. Thus, the P. multocida mutant with mutation of the gatA gene and part of the hptE gene proved to be an effective live attenuated vaccine candidate for prevention of fowl cholera in ducks.

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Characterization of Pasteurella multocida isolated from ducks in China from 2017 to 2019

Cited by 9 publications

References 52 publications

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

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