Characterization of P450 monooxygenase gene family in the cotton aphid, Aphis gossypii Glover

Zhao, Likang; Wang, Chuanpeng; Gao, Xueke; Luo, Junyu; Zhu, Xiangzhen; Wan, Sumei

doi:10.1016/j.aspen.2021.101861

Cited by 4 publications

(4 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aphids use a complex combination of enzymes to avoid the plant defense chemicals, including cytochrome P450 (CYP), glutathione transferases, carboxylesterase, UDP‐glucuronosyltransferase, oxidoreductases, peroxidases, superoxide dismutase, and others. CYP enzymes are one of the most extensive gene families found in living organisms, distinguished by their heme thiolate group and engaged in a variety of functions such as xenobiotic and endogenous metabolism (Werck‐Reichhart and Feyereisen, 2000; Zhao et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap‐sucking insects

Pavithran,

Murugan,

Mannu

et al. 2024

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Aphids are sap‐sucking insects responsible for crop losses and a severe threat to crop production. Proteins in the aphid saliva are integral in establishing an interaction between aphids and plants and are responsible for host plant adaptation. The cotton aphid, Aphis gossypii (Hemiptera: Aphididae) is a major pest of Gossypium hirsutum. Despite extensive studies of the salivary proteins of various aphid species, the components of A. gossypii salivary glands are unknown. In this study, we identified 123,008 transcripts from the salivary gland of A. gossypii. Among those, 2933 proteins have signal peptides with no transmembrane domain known to be secreted from the cell upon feeding. The transcriptome includes proteins with more comprehensive functions such as digestion, detoxification, regulating host defenses, regulation of salivary glands, and a large set of uncharacterized proteins. Comparative analysis of salivary proteins of different aphids and other insects with A. gossypii revealed that 183 and 88 orthologous clusters were common in the Aphididae and non‐Aphididae groups, respectively. The structure prediction for highly expressed salivary proteins indicated that most possess an intrinsically disordered region. These results provide valuable reference data for exploring novel functions of salivary proteins in A. gossypii with their host interactions. The identified proteins may help develop a sustainable way to manage aphid pests.

show abstract

Section: Discussionmentioning

confidence: 99%

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap‐sucking insects

Pavithran,

Murugan,

Mannu

et al. 2024

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…Firstly, the technology of sequencing affects the number of P450 genes; for example, 35 and 34 P450 genes were identified based on two transcriptomic data sets of the cotton aphid A. gossypii , respectively [ 55 , 56 ]. In contrast, 49 P450 genes were identified in a study of the A. gossypii genome [ 42 ]. Our current data are based on the latest sequencing technology, which can reduce the effect of sequencing technology.…”

Section: Discussionmentioning

confidence: 99%

“…The numbers of P450 genes from different insect species, including A. pisum , the green peach aphid Myzus persicae , the cotton aphid Aphis gossypii , the soybean aphid Aphis glycines , the silkworm Bombyx mori , D. melanogaster , Anopheles gambiae , the oriental fruit fly Bactrocera dorsalis , Apis mellifera, and the red flour beetle Tribolium castaneum , were collected through previous studies and the numbers of genes contained in each clan were listed in detail [ 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. These species include a number of model insects and some closely related aphid species.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Zhang

Wang

et al. 2023

Insects

View full text Add to dashboard Cite

Cytochrome P450 monooxygenases (P450s) have a variety of functions, including involvement in the metabolism of exogenous substances and the synthesis and degradation of endogenous substances, which are important for the growth and development of insects. Pseudoregma bambucicola is a social aphid that produces genetically identical but morphologically and behaviorally distinct first-instar soldiers and normal nymphs within colonies. In this study, we identified 43 P450 genes based on P. bambucicola genome data. Phylogenetic analysis showed that these genes were classified into 4 clans, 13 families, and 23 subfamilies. The CYP3 and CYP4 clans had a somewhat decreased number of genes. In addition, differential gene expression analysis based on transcriptome data showed that several P450 genes, including CYP18A1, CYP4G332, and CYP4G333, showed higher expression levels in soldiers compared to normal nymphs and adult aphids. These genes may be candidates for causing epidermal hardening and developmental arrest in soldiers. This study provides valuable data and lays the foundation for the study of functions of P450 genes in the social aphid P. bambucicola.

show abstract

“…Cytochrome P450 monooxygenase (CYP) is a supergene family enzyme system that widely exists in eukaryotes [23][24][25]. It not only has metabolic effects on exogenous substances such as pesticides, plant secondary substances, and other environmental toxic substances, but also participates in some endogenous substances that play important physiological functions such as the hormone metabolism of steroids and fatty acids [26][27][28]. Among the four gene clusters of the CYP gene family, CYP2 and mito genes are mainly involved in the growth and development of insects, such as the biosynthesis of ecdysteroids, etc.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the TcCYPE2 Gene and Its Role in Regulating Trehalose Metabolism in Response to High CO2 Stress

Zhou,

Wang

et al. 2023

Agronomy

View full text Add to dashboard Cite

Cytochrome P450 monooxygenase (CYP) is one of the three detoxification metabolic enzymes in insects, and is involved in the metabolism and transformation of endogenous substances as well as the activation and degradation of exogenous compounds. This study aims to reveal the molecular mechanism of CYP9E2 in Tribolium castaneum in adapting to high-CO2 stress. By predicting the sequence function of CYP9E2, analyzing the temporal and spatial expression profile of TcCYP9E2, and using RNAi to silence TcCYP9E2 combined with a high-CO2 stress treatment, we measured the carbohydrate content, trehalase activity, and gene expression levels in trehalose metabolism of T. castaneum. A bioinformatics analysis showed that the predicted molecular weight of the protein encoded by TcCYP9E2 is 60.15, the theoretical isoelectric point is 8.63, there is no signal peptide, and the protein is hydrophilic. An evolutionary tree analysis showed that TcCYP9E2 belongs to the CYP6 family and belongs to the CYP3 group; and the spatiotemporal expression profile results showed that TcCYP9E2 was highly expressed in the larvae midgut 48 h after injection of dsCYP9E2, with survival rates decreasing with the increase in CO2 concentration. Under the condition of 75% CO2, the contents of glycogen, glucose, ATP, and membrane-bound trehalase decreased significantly after the injection of dsCYP9E2. The expression of TRE-1, TRE-2, and GP in trehalose metabolism and energy pathways was significantly downregulated.

show abstract

Characterization of P450 monooxygenase gene family in the cotton aphid, Aphis gossypii Glover

Cited by 4 publications

References 78 publications

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap‐sucking insects

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap‐sucking insects

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Characterization of the TcCYPE2 Gene and Its Role in Regulating Trehalose Metabolism in Response to High CO2 Stress

Contact Info

Product

Resources

About