Characterization of oxidized phospholipids in oxidatively modified low density lipoproteins by nanoflow liquid chromatography–tandem mass spectrometry

Lee, Ju Yong; Lim, Sangsoo; Park, Sungha; Moon, Myeong Hee

doi:10.1016/j.chroma.2013.02.086

Cited by 17 publications

(15 citation statements)

References 31 publications

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“…Oxidation of a phospholipid molecule usually occurs by abstraction of a hydrogen atom from a bis-allylic methylene of a polyunsaturated fatty acid either via an inorganic reactive oxygen species (ROS) or enzymatic oxidation [49]. LDL oxidation induces apo B containing intrinsic PLA 2 activity [2,50].…”

Section: Pc Species/lpc Speciesmentioning

confidence: 99%

Human native, enzymatically modified and oxidized low density lipoproteins show different lipidomic pattern

Orsó

Matysik

Grandl

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Section: Pc Species/lpc Speciesmentioning

confidence: 99%

Human native, enzymatically modified and oxidized low density lipoproteins show different lipidomic pattern

Orsó

Matysik

Grandl

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars their analysis and available MS data grow, this is being remedied. LiPilot is a software tool designed for the identification of phospholipid structures from MSMS data (64) and has been used to carry out automatic searches of oxidized PLs found in LDL (60). This is an area that is expected to develop greatly in the next few years, and will be very important as improvement in instrument technology and scanning rates provide ever-increasing amounts of data, for example from ''pseudo-targeted'' approaches using q-TOF instruments, which requires data mining of the extensive datasets.…”

Section: Future Perspectivesmentioning

confidence: 99%

Oxidative Lipidomics Coming of Age: Advances in Analysis of Oxidized Phospholipids in Physiology and Pathology

Spickett

Pitt

2015

Antioxidants & Redox Signaling

100

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Significance: Oxidized phospholipids are now well recognized as markers of biological oxidative stress and bioactive molecules with both pro-inflammatory and anti-inflammatory effects. While analytical methods continue to be developed for studies of generic lipid oxidation, mass spectrometry (MS) has underpinned the advances in knowledge of specific oxidized phospholipids by allowing their identification and characterization, and it is responsible for the expansion of oxidative lipidomics. Recent Advances: Studies of oxidized phospholipids in biological samples, from both animal models and clinical samples, have been facilitated by the recent improvements in MS, especially targeted routines that depend on the fragmentation pattern of the parent molecular ion and improved resolution and mass accuracy.

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“…Formation of lipid vesicles was conducted by a published method [ 37 ]. In brief, 10 μg of 16:0-LPC, 16:0-PE, 16:0/14:0-PC, 18:0/18:1-PC, 18:0/18:1/18:1-TAG, 16:0/16:0/14:1-TAG, 16:018:1-DAG, 16:0/16:0-PA (totally, 8 species of lipid standards) were dissolved in the organic solvent mixture of CH 3 Cl and MeOH (8:2, v/v).…”

Section: Methodsmentioning

confidence: 99%

Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry

et al. 2018

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Nanoparticles (NPs) tend to adsorb matrix molecules like proteins and lipids incubated with biological fluids, forming a biological corona. While the formation and functions of protein corona have been studied extensively, little attention has been paid to lipid adsorption on NPs. However, lipids are also abundantly present in biological fluids and play important roles in processes like cell signaling and angiogenesis. Therefore, in this study, we established the analytical procedure for study of lipid adsorption on three different types of NPs in two matrices: human serum and heavy cream, using nanoflow liquid chromatography-mass spectrometry (nanoflowLC-MS). Serum was chosen to represent the common environment the NPs would be present once entering human body, and heavy cream was the representative food matrix NPs may be added to improve the color or taste. Steps of liquid-liquid extraction were established and optimized to achieve maximum recovery of the adsorbed, standard lipids from the NPs. Then, the LC-MS/MS method was developed to attain base-line separation of the standard lipids that represent the major lipid classes. At last, the lipid adsorption profiles of the three NPs were compared. We found that the lipid adsorption profile on the same type of NP was significantly different between the two matrices. The established method will help us investigate lipid adsorption on additional NPs and reveal how it could be affected by the physiochemical properties of NPs and the presence of proteins and other components in the biological matrix.

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Characterization of oxidized phospholipids in oxidatively modified low density lipoproteins by nanoflow liquid chromatography–tandem mass spectrometry

Cited by 17 publications

References 31 publications

Human native, enzymatically modified and oxidized low density lipoproteins show different lipidomic pattern

Human native, enzymatically modified and oxidized low density lipoproteins show different lipidomic pattern

Oxidative Lipidomics Coming of Age: Advances in Analysis of Oxidized Phospholipids in Physiology and Pathology

Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry

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