Characterization of ovine dermal papilla cell aggregation

Abstract

Context:The dermal papilla (DP) is a condensation of mesenchymal cells at the proximal end of the hair follicle, which determines hair shaft size and regulates matrix cell proliferation and differentiation. DP cells have the ability to regenerate new hair follicles. These cells tend to aggregate both in vitro and in vivo. This tendency is associated with the ability of papilla cells to induce hair growth. However, human papilla cells lose their hair-inducing activity in later passage number. Ovine DP cells are different from human DP cells since they do not lose their aggregative behavior or hair-inducing activity in culture. Nonetheless, our understanding of ovine DP cells is still limited.Aim:The aim of this study was to observe the expression of established DP markers in ovine cells and their association with aggregation.Subjects and Methods:Ovine DP cells from three different sheep were compared. Histochemistry, immunoflourescence, and polymerase chain reaction experiments were done to analyze the DP markers.Results:We found that ovine DP aggregates expressed all the 16 markers evaluated, including alkaline phosphatase and versican. Expression of the versican V0 and V3 isoforms, neural cell adhesion molecule, and corin was increased significantly with aggregation, while hey-1 expression was significantly decreased.Conclusions:Overall, the stable expression of numerous markers suggests that aggregating ovine DP cells have a similar phenotype to papillae in vivo. The stability of their molecular phenotype is consistent with their robust aggregative behavior and retained follicle-inducing activity after prolonged culture. Their phenotypic stability in culture contrasts with DP cells from other species, and suggests that a better understanding of ovine DP cells might provide opportunities to improve the hair-inducing activity and therapeutic potential of human cells.

Ti0.8O2Nanosheets Inhibit Lung Cancer Stem Cells by Inducing Production of Superoxide Anion

“…Ti 0.8 O 2 Nanosheets Have No Effect on Primary Dermal-Papilla Aggregation Properties. The data thus far has indicated that DP aggregation patterns reflect the ability to form hair (Sari et al, 2016). Having shown the promising effect of Ti 0.8 O 2 nanosheets in selective suppression of the CSC in lung cancer, we next evaluated whether treatment of normal cells with such a substance affected the viability of normal cells as well as their stem cell properties.…”

Horse hair follicles: A novel dermal stem cell source for equine regenerative medicine

“…The stable detection of versican in the epidermis, specifically in the stratum germinativum as described by Zimmermann et al , could be observed in all tested skin samples. The study in ovine cell aggregates harvested from secondary wool hair follicles reported versican loss in adherent cells . Unlike in ovine, our equine adherent cells clearly expressed versican.…”

Hair Growth Promoting Effect of 4HGF Encapsulated with PGA Nanoparticles (PGA-4HGF) by β-Catenin Activation and Its Related Cell Cycle Molecules

“…DPCs play a key role in generating hair bulbs and regulating hair-growth [5,47]. They are known to aggregate reactions that induce HF formation, and determine the hair bulb and shaft due to stem cell-like plasticity [48,49,50,51,52]. Therefore, the proliferation and stemness of DPCs can increase hair-growth.…”