Characterization of outer membrane and secreted proteins of Leptospira interrogans serovar pomona

Characterization of the Leptospiral Outer Membrane and Description of Three Novel Leptospiral Membrane Proteins

“…However, spirochetal architecture differs significantly from that of gram-negative bacteria in that the peptidoglycan layer of spirochetes is associated with the cytoplasmic membrane rather than the OM (22,24). For this reason, techniques developed for isolation of the gram-negative OM from components of the underlying cell wall and cytoplasmic membrane are unlikely to be useful in spirochetes.Several approaches have been used in the isolation of the leptospiral OM, including sodium dodecyl sulfate (SDS) treatment of salt-altered cells (2,7,25,28), Sarkosyl (27), and Triton X-114 extraction and phase partitioning (20,45). On the basis of these approaches, three classes of leptospiral OM proteins (OMPs) have been described: transmembrane, lipoprotein, and peripheral membrane proteins.…”

Amino acid sequences of proteins from Leptospira serovar pomona

“…Investigators isolated and characterized the outer envelope proteins in the range of 22 to 66 kDa from Leptospira interrogans using different detergent and extraction methods (Nunes et al 1985, Brown et al 1991, Haake et al 1991, Zuerner et al 1991. Haake et al (1991) and Zuerner et al (1991) used Triton X-114 extraction to isolate and characterize outer envelope antigens of serovar grippotyphosa and pomona, respectively. They concluded that these detergent phase proteins were related to integral membrane proteins.…”

“…Host immune defense mechanisms in leptospirosis are mainly humoral (Adler et al 1980) with production of specific antibodies primarily to the outer envelope antigens. Whole cell (Bey 1982) and outer envelope antigens (Auran et al 1972, Zuerner et al 1991 have been extracted from leptospiras by different methods and proven to be immunogenic. Investigators isolated and characterized the outer envelope proteins in the range of 22 to 66 kDa from Leptospira interrogans using different detergent and extraction methods (Nunes et al 1985, Brown et al 1991, Haake et al 1991, Zuerner et al 1991.…”

“…Whole cell (Bey 1982) and outer envelope antigens (Auran et al 1972, Zuerner et al 1991 have been extracted from leptospiras by different methods and proven to be immunogenic. Investigators isolated and characterized the outer envelope proteins in the range of 22 to 66 kDa from Leptospira interrogans using different detergent and extraction methods (Nunes et al 1985, Brown et al 1991, Haake et al 1991, Zuerner et al 1991. Haake et al (1991) and Zuerner et al (1991) used Triton X-114 extraction to isolate and characterize outer envelope antigens of serovar grippotyphosa and pomona, respectively.…”

Avaliação e caracterização de candidatos vacinais voltados para o controle da leptospirose.

“…Esse detergente, inicialmente, permite a solubilização da membrana externa das bactérias sem promover alteração na membrana citoplasmática e, com isso, duas frações são obtidas (ZUERNER et al, 1991). Uma dessas frações, a solúvel ao detergente, pode ainda ser separada em duas fases após a elevação de temperatura, o que resulta em uma fase hidrofóbica rica em detergente (fase DET) e uma fase aquosa com pouco detergente (fase AQ).…”