Characterization of OsPM19L1 encoding an AWPM-19-like family protein that is dramatically induced by osmotic stress in rice

Chen, H; Lan, Haiyan; Huang, Peng; Zhang, Y; Yuan, Xunlai; Huang, Xiaosan; Huang, Ji; Zhang, H

doi:10.4238/2015.october.5.12

Cited by 23 publications

(36 citation statements)

References 33 publications

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“…The colour assignments are: In the model plant Arabidopsis thaliana, AWPM19 proteins are encoded for by a 4-member gene family (PM19L1-4, 8 ). We confirmed plasma membrane localisation of PM19L1 protein 3,6,7 by confocal imaging of cells from transgenic Arabidopsis seedlings expressing free GFP or a PM19L1-GFP fusion driven by the CaMV 35S promoter. PM19L1-GFP exhibited colocalization with FM4-64 at the plasma membrane ( Fig.…”

Section: Results Colour-coded For Amino Acid Conservationsupporting

confidence: 58%

The conserved Arabidopsis PM19L1 protein functions as an osmosensor and regulator of dormancy and germination

Alexander¹,

Castillejo-Pons

Alsaif

et al. 2020

Preprint

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During germination, seeds undergo a dramatic transformation in their water content, which increases from around 10% to 90% within 24 hours. Control of water uptake to the embryo is key to the germination process; failure to imbibe sufficiently can result in seed dormancy. This is strongly affected by environmental factors including drought or salinity and can result in reduced crop establishment and yields. However, the osmosensing mechanisms regulating water uptake in plants have not been described. In this work we show that the highly conserved plant plasma membrane protein AWPM19 is a plant osmosensor, regulating germination under osmotic stress. The AWPM19 protein structurally resembles the yeast osmosensor Sho1, with four transmembrane domains, and the Arabidopsis homologue PM19L1 will phenotypically complement the sho1 mutant, showing that it can function as an osmosensor. We find that PM19L1 is highly expressed in the seed and seedling during germination and is located in the plasma membrane. Arabidopsis pm19l1 mutants have reduced germination in the presence of salt or sorbitol. In a striking parallel to osmosensing in yeast, signalling downstream of AWPM19 involves a MAP kinase signal transduction pathway. These results give an indication of how plants are able to sense the availability of water, and has important implications for the study of dormancy, drought and salinity tolerance in crops. Furthermore this provides insight into evolutionary adaptation of plants to a terrestrial environment.

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Section: Results Colour-coded For Amino Acid Conservationsupporting

confidence: 58%

The conserved Arabidopsis PM19L1 protein functions as an osmosensor and regulator of dormancy and germination

Alexander¹,

Castillejo-Pons

Alsaif

et al. 2020

Preprint

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“…It plays important role in hair growth and development and in the P deficiency induced phospholipid degradation pathway in wheat roots and also found in Lupinus albus (Cheng et al, 2011). AWPM-19-like membrane family protein encoded by 2 transcripts in our study plays important roles in plant development and various stress responses (Chen et al, 2015). It has a similar sequence of LEA protein, which is induced by ABA during freezing tolerance (Koike et al, 1997).…”

Section: Resultsmentioning

confidence: 77%

Transcriptomic Analysis of Soil Grown T. aestivum cv. Root to Reveal the Changes in Expression of Genes in Response to Multiple Nutrients Deficiency

et al. 2017

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Deficiency of necessary macronutrients, i.e., Potassium (K), Magnesium (Mg), Nitrogen (N), Phosphorus (P), and Sulfate (S) in the soil leads to a reduction in plant growth and yield, which is a result of changes in expression level of various genes. This study was performed to identify the differentially expressed genes and its associated metabolic pathways occurred in soil grown wheat root samples excavated from the control and treated fields. To identify the difference in gene expression levels due to deficiency of the said nutrients, a transcriptomic, meta-analysis was performed on array expression profile data. A set of 435 statistically significant probes encoding 398 Nutrient Deficiency Response Genes (NRGs) responding at-least one nutrients deficiency (ND) were identified. Out of them 55 NRGs were found to response to minimum two ND. Singular Enrichment Analysis (SEA) predicts ontological based classifications and functional analysis of NRGs in different cellular/molecular pathways involved in root development and growth. Functional annotation and reaction mechanism of differentially expressed genes, proteins/enzymes in the different metabolic pathway through MapMan analysis were explored. Further the meta-analysis was performed to revels the active involvement each NRGs in distinct tissues and their comparative potential expression analysis in different stress conditions. The study results in exploring the role of major acting candidate genes such as Non-specific serine/threonine protein kinase, Xyloglucan endotransglucosylase/hydrolase, Peroxides, Glycerophosphoryl diester phosphodiesterase, S-adenosylmethionine decarboxylase proenzyme, Dehydrin family proteins, Transcription factors, Membrane Proteins, Metal binding proteins, Photosystem proteins, Transporter and Transferase associated in different metabolic pathways. Finally, the differences of transcriptional responses in the soil-grown root of T. aestivum cv. and in-vitro grown model plants under nutrients deficiency were summarized.

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“…As involvement of ABA in the control of seed dormancy is well established, as well as in bud dormancy (González‐Grandío and Cubas, ; Shu et al ., ; Nguyen and Emery, ), we further analyzed the effects of ABA in the control of SL‐mediated axillary bud dormancy in rice. Indeed, the gene most highly activated in the dormant buds was an ortholog of AWPM‐19 , which is an ABA‐responsive gene of unknown function isolated from Arabidopsis and was recently shown to be involved in seed dormancy of wheat (Barrero et al ., ; Chen et al ., ) (Figure a; Table S3). The AWPM‐19 gene of rice was strongly expressed in the youngest leaf primordia in the dormant bud, suggesting that the ABA level may be raised in the leaf primordia prior to the onset of dormancy (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

et al. 2019

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SummaryBy contrast with rapid progress in understanding the mechanisms of biosynthesis and signaling of strigolactone (SL), mechanisms by which SL inhibits axillary bud outgrowth are less well understood. We established a rice (Oryza sativa L.) hydroponic culture system to observe axillary buds at the critical point when the buds enter the dormant state. In situ hybridization analysis indicated that cell division stops in the leaf primordia of the buds entering dormancy. We compared transcriptomes in the axillary buds isolated by laser capture microdissection before and after entering the dormant state and identified genes that are specifically upregulated or downregulated in dormant buds respectively, in SL‐mediated axillary bud dormancy. Typically, cell cycle genes and ribosomal genes are included among the active genes while abscisic acid (ABA)‐inducible genes are among the dormant genes. Application of ABA to the hydroponic culture suppressed the growth of axillary buds of SL mutants to the same level as wild‐type (WT) buds. Tiller number was decreased in the transgenic lines overexpressing OsNCED1, the gene that encodes ABA biosynthesis enzyme. These results indicated that the main site of SL function is the leaf primordia in the axillary bud and that ABA is involved in SL‐mediated axillary bud dormancy.

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Characterization of OsPM19L1 encoding an AWPM-19-like family protein that is dramatically induced by osmotic stress in rice

Cited by 23 publications

References 33 publications

The conserved Arabidopsis PM19L1 protein functions as an osmosensor and regulator of dormancy and germination

The conserved Arabidopsis PM19L1 protein functions as an osmosensor and regulator of dormancy and germination

Transcriptomic Analysis of Soil Grown T. aestivum cv. Root to Reveal the Changes in Expression of Genes in Response to Multiple Nutrients Deficiency

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

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