Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study

Laliberte, Catherine; Ng, Nicole; Eymael, Denise; Higgins, Kevin; Ali, Aiman; Kiss, Alex; Bradley, Grace; Magalhães, Marco A. O.

doi:10.3389/froh.2021.740469

Cited by 13 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data are in concordance with previous reports detecting higher levels of IL10, IP-10 (CXCL10), and IL-8 in peripheral blood of HNSCC compared to HD ( 7 – 9 ). Yet, the HIS stratification model illustrated in the current work revealed differential expression of IL10 in the HIS-INF, and IP-10, and IL8 in HIS-IE compared to HD.…”

Section: Discussionsupporting

confidence: 93%

Cytokine profiling in plasma distinguishes the histological inflammatory subtype of head and neck squamous cell carcinoma and a novel regulatory role of osteopontin

Ghita

Piperi

Atamas

et al. 2022

Front. Oral. Health

View full text Add to dashboard Cite

Head and neck squamous cell carcinoma (HNSCC) can be classified according to the histological inflammatory subtype (HIS) into inflamed (HIS-INF) or immune excluded (HIS-IE). HIS-IE was previously associated with higher levels of soluble Semaphorin 4D (HsS4D) in plasma, and higher transcriptional levels of osteopontin (OPN) in the tumor tissue, compared to HIS-INF. The goal of the current study is to investigate whether the HIS inflammatory subtype can be distinguished by a differential cytokine panel in peripheral blood. Retrospectively collected five HIS-INF and five HIS-IE tumor tissue with paired plasma were included in the study. Five healthy donors (HD) and five autoimmune/chronic inflammatory conditions (AI/CI) were controls. The ELISA-Luminex™ system was used to detect 40 traditional cytokines in plasma. Human cytokine array (104 cytokines) was used for the conditioned medium (CM) of the HNSCC HN6 cell line. Semaphorin 4D (Sema4D) siRNA and recombinant human osteopontin (rh-OPN) were used to investigate the effect of OPN on Sema4D expression. The HIS-IE cytokine profile was higher than HIS-INF but comparable to AI/CI. HIS-INF had the lowest cytokine levels. HIS-IE was differentially higher in IP-10 and IL8 compared to HD, while HIS-INF was higher in IL-10. Sema4D inhibition in HN6 resulted in a decrease of OPN in the CM of HN6, and treatment with rh-OPN rescued Sema4D in HN6 cell lysate and associated CM. In conclusion, the current work demonstrates a novel association between the HIS subtypes and a differential pattern of cytokine expression in plasma. These findings can open new avenues for HNSCC patient stratification and hence provide better personalized treatment.

show abstract

Section: Discussionsupporting

confidence: 93%

Cytokine profiling in plasma distinguishes the histological inflammatory subtype of head and neck squamous cell carcinoma and a novel regulatory role of osteopontin

Ghita

Piperi

Atamas

et al. 2022

Front. Oral. Health

View full text Add to dashboard Cite

show abstract

“…Furthermore, despite the questionable relationship between chronic in ammation and carcinogenesis, recent studies suggest that it could promote genetic and epigenetic changes that might lead to the development of oral SCC. [30][31][32] The presence of epithelial dysplasia is reported in the literature in 0.5-25% of OLP cases 12,21,33,34 and 5.6-27.2% of LLO cases. 21,33 Concerning the degree of epithelial dysplasia, Vijayan and Muthukrishnan 35 in 2022 found that out of 250 OLP lesions, 14 (5.6%) exhibited epithelial dysplasia, with 12 having mild epithelial dysplasia and 2 moderate.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Controversy: Dysplasia in Oral Lichen Planus - A Comparative Study Based on WHO Criteria and the Binary System

Marques,

Lopes,

Gonçalves

et al. 2024

Preprint

View full text Add to dashboard Cite

Background To assess the presence and degree of epithelial dysplasia of epithelial dysplasia according to the World Health Organization (WHO) criteria and the binary system in oral lichen planus (OLP) and oral lichenoid lesions (OLL), and to compare the influence of individual architectural and cytological criteria on the assessment of the degree of epithelial dysplasia in these lesions. Methods Sixty-five biopsies from lesions diagnosed as OLP and OLL underwent evaluation by two oral pathologists to diagnose oral epithelial dysplasia. This assessment utilized both WHO criteria and the binary system, with consideration given to individual architectural and cytological criteria in the diagnostic process. Results All biopsies showed epithelial dysplasia, with the majority classified as mild epithelial dysplasia according to WHO criteria (73.8%) and low risk by the binary system (61.5%). There was a statistically significant association in the classification of epithelial dysplasia between WHO criteria and the binary system. No statistically significant differences were found in the association of the presence and degree of epithelial dysplasia with the diagnosis of OLP and LLO. Statistical analysis indicated that an increase in the number of mitotic figures was associated with the severity of epithelial dysplasia (moderate/severe) according to the WHO system. Drop-shaped projections of epithelial ridges, an increased number of mitotic figures, superficial mitoses, premature keratinization in single cells, abnormal variation in cell shape, and atypical mitotic figures were associated with the high risk by the binary system. Conclusion The presence of epithelial dysplasia is common in both oral lichen planus and oral lichenoid lesions, and the degree of epithelial dysplasia does not statistically differ between these lesions. The absence of epithelial dysplasia should not be considered a diagnostic criterion for classifying OLP. The binary system may provide a more precise assessment of epithelial dysplasia in OLP and LLO lesions.

show abstract

“…Other studies have shown that there is a significant pro-inflammatory immune environment in OSCC. [17] In the tumor microenvironment, the production of a variety of pro-inflammatory cytokines can promote the survival and proliferation of tumor cells. [18,19] CDSN is located in the core of desmosomes in the cuticle.…”

Section: Discussionmentioning

confidence: 99%

Corneodesmosin as a potential target of oral squamous cell carcinoma

Bao

Guo

et al. 2022

Medicine

View full text Add to dashboard Cite

Objective: The relationship between oral squamous cell carcinoma (OSCC) and Corneodesmosin (CDSN) remains unclear. This study aims to explore the correlation between CDSN and the prognosis and survival time of patients with OSCC. Methods: Bioinformatics were used to identify the hub role of CDSN in the OSCC. A total of 200 patients with OSCC were recruited. Clinical and follow-up data were recorded, and the expression level of CDSN was detected. Pearson chi-square test and Spearman correlation coefficient were used to analyze the relationship between prognosis and related parameters in patients with OSCC. Univariate and multivariate Logistic regression and Cox proportional risk regression were applied for further analysis, and receiver operating characteristic curve and survival curve of subjects were plotted. Results: CDSN was identified as the most significant hub gene of the OSCC by the cytoHubba. By the comparative toxicogenomics database (CTD) analysis, there was strong relationship between the CDSN and mouth neoplasms, head and neck neoplasms, squamous cell carcinoma of head and neck. The OSCC patients with low expression level of CDSN have poor overall survival compared with the high expression level of CDSN (HR = 0.75, 95% confidence interval [95%CI]: 0.57-0.98, P = .036). Spearman correlation coefficient analysis showed that CDSN expression level was significantly correlated with prognosis (ρ = -0.528, P < .001). Multivariate Logistic regression analysis showed that poor prognosis (odds ratio [OR] = 0.096, 95%CI: 0.049-0.189, P < .001) was significantly associated with low expression of CDSN. Cox regression analysis showed that the survival time of OSCC patients was shorter when CDSN expression was low (HR = 0.588, 95%CI: 0.420-0.823, P = .002). Strong predictive value of CDSN for the OSCC survival time was obtained by the biological process (BP)-neural network and support vector machine (SVM). Conclusion: CDSN was significantly correlated with OSCC, and the shorter the survival time of patients with OSCC was, the worse the prognosis was.

show abstract

Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study

Cited by 13 publications

References 32 publications

Cytokine profiling in plasma distinguishes the histological inflammatory subtype of head and neck squamous cell carcinoma and a novel regulatory role of osteopontin

Cytokine profiling in plasma distinguishes the histological inflammatory subtype of head and neck squamous cell carcinoma and a novel regulatory role of osteopontin

Exploring the Controversy: Dysplasia in Oral Lichen Planus - A Comparative Study Based on WHO Criteria and the Binary System

Corneodesmosin as a potential target of oral squamous cell carcinoma

Contact Info

Product

Resources

About