Characterization of olive seed storage proteins

Abstract

At present little is known about olive seed storage proteins (SSPs). A better understanding of olive SSPs will be important for future biotechnology efforts. In the present study, we first developed a protocol relied on chloroform for preparing protein samples free of lipids from lipid-rich olive seeds. Then, we characterized olive SSPs by SDS-PAGE, N-terminal sequencing and immunoblot. Two smaller subunits (20 and 21.5 kD) of SSPs were purified to homogeneity and used for antibody production or N-terminal sequencing. N-terminal sequencing confirmed that major olive SSPs are 11S globulins. Moreover, the components and size distribution of SSPs are identical among several olive cultivars examined, suggesting that their synthesis is highly conserved in this species. Olive SSPs are soluble in aqueous alcohol, with limited solubility in water and dilute salt. Thus, despite their homology with globulins, olive SSPs are similar in solubility to prolamins and different from globulins in other dicot plants. Finally, the accumulation of olive SSPs during fruit maturation was examined. Our results revealed that the accumulation of SSPs is time-dependent and tissue-specific, and only 105 days after pollination (DAP), did individual components of SSPs synthesize substantially, and accumulate rapidly in large quantities over a short period of time. Our results suggest that a 36 kD protein is the precursor of olive SSPs, and 90-105 DAP seems to be a crucial transition period (from a precursor to mature subunits) for the accumulation of SSPs.

Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaeaL.) Seeds

“…Previous studies by our research group have demonstrated that 11S SSPs similar to legumins are extremely abundant in the olive endosperm and embryo and are localized in numerous protein bodies present in the cytoplasm of cells, which are part of these tissues [ 8 , 12 ]. In the olive seed, 11S proteins have been biochemically characterised, and the evolution of their olive seed formation and germination has been determined [ 8 , 13 , 14 ].…”

Recovery of Nitrogen from Low-Cost Plant Feedstocks Used for Bioenergy: A Review of Availability and Process Order

“…Several protein extraction methodologies and conditions have been reported for integrated biorefineries. , Chemical methods include alkaline extraction, , aqueous ammonia (AFEX) extraction, , buffer extraction, organic solvent extraction, and combinations of these methods. , Alkaline extraction is commonly used for extracting proteins from agriculture residues, deoiled seedcakes, and DDGS with protein recoveries of 82–91.1%, 15–36%, and 60–79%, respectively. − The efficacy of protein extraction using chemical methods depends on the nature of the biomass, solid-to-solvent ratio, temperature, pH, and process time . A recent review by Kumar et al summarized the effects of the sample-to-solvent ratio, alkali concentration, time, and temperature for several protein extractions from plant .…”

The Potential of Tree Fruit Stone and Seed Wastes in Greece as Sources of Bioactive Ingredients

“…Currently, scientific research has focused more on the compositional characteristics of the wooden part of olive stones with regard to its heating or composting potential [25,26] rather than on the biochemical profile of the olive stones as a whole or of the olive seeds alone. The whole olive stone seems to be a good source of bioactive compounds [11,[27][28][29][30][31]. Phenols constitute the most important family of bioactive constituents in the olive seeds, as it is the case with other parts of the olive drupe.…”