Characterization of olfactory sensilla of Stomoxys calcitrans and electrophysiological responses to odorant compounds associated with hosts and oviposition media

Abstract

Brewer, Gary J.; and Zhu, J. J., "Characterization of olfactory sensilla of Stomoxys calcitrans and electrophysiological responses to odorant compounds associated with hosts and oviposition media" (2011). Faculty Publications: Department of Entomology. 338. http://digitalcommons.unl.edu/entomologyfacpub/338 Medical and Veterinary Entomology (2011) 25, 327-336 doi: 10.1111/j.1365-2915.2011 Characterization of olfactory sensilla of Stomoxys calcitrans and electrophysiological responses to odorant compounds associated with hosts and oviposition media To further our understanding of stable fly olfaction, we examined the antennal morphology of adults using scanning electron microscopy techniques. Four major types of sensillum were found and classified as: (a) basiconic sensilla; (b) trichoid sensilla with three subtypes; (c) clavate sensilla, and (d) coeloconic sensilla. No significant differences between male and female flies in abundances (total numbers) of these sensillum types were observed, except for medium-sized trichoid sensilla. The distinctive pore structures found on the surface of basiconic and clavate sensilla suggest their olfactory functions. No wall pores were found in trichoid and coeloconic sensilla, which suggests that these two types of sensillum may function as mechano-receptors. Details of the distributions of different sensillum types located on the funicle of the fly antenna were also recorded. Electroantennogram results indicated significant antennal responses to host-associated compounds. The importance of stable fly olfaction relative to host and host environment seeking is discussed. This research provides valuable new information that will enhance future developments in integrated stable fly management.

Olfactory sensilla on antennae and maxillary palps of Fannia hirticeps (Stein, 1892) (Diptera: Fanniidae)

“…The funicular surface of F. hirticeps appears densely populated by a large number of trichoid and basiconic sensilla and less common coeloconic and clavate sensilla. These sensilla types and morphology are similar to those of other fly species such as Chrysomya megacephala , C. rufifacies , Lucilia cuprina (Sukontason et al,2004), Stomoxys calcitrans (Tangtrakulwanich et al,2011), Musca domestica (Sukontason et al,2004,2008), Musca autumnalis (Bay and Pitts,1976), Hydrotaea chalcogaster (Sukontason et al,2006), Delia radicum , D. floralis , D. antiqua , D. plature (Ross,1992), and Drosophila melanogaster (Shanbhag et al,1999). Except for coeloconic sensilla, the funicular sensilla of F .…”

Spatial-Temporal Dynamics of Stable Fly (Diptera: Muscidae) Trap Catches in Eastern Nebraska

“…Larval developmental sites for stable ßies, as with most ßies, are ephemeral and may be limiting resources with respect to population growth. Stable ßies orient toward potential larval developmental sites by using olfactory cues (Tangtrakulwanich et al 2011) and disperse from them upon completing immature development (Taylor et al 2010). Movement of hosts and availability of carbohydrate sources may be contributing to reduced synchrony of trap catches as well.…”

Identification of stable fly attractant compounds in vinasse, a byproduct of sugarcane–ethanol distillation

“…Vinasse has a very distinct odour that can be perceived from a distance, primarily as a result of its high content of short‐chain organic acids. These short‐chain acids include butanoic acid, hexanoic acid, 3‐methyl butanoic acid and pentanoic acid, and have been demonstrated previously to attract S. calcitrans , which shows strong EAG responses (Jeanbourquin & Guerin, 2007a, 2007b; Tangtrakulwanich et al ., ). The attraction of S. calcitrans to vinasse was further confirmed in the field test, in which six times as many stable flies were captured in traps using vinasse as a lure than in traps using any other test material.…”