Characterization of nutrients, amino acids, polyphenols and antioxidant activity of Ridge gourd (Luffa acutangula) peel

Swetha, M.; Muthukumar, S. P.

doi:10.1007/s13197-016-2285-x

Cited by 20 publications

(17 citation statements)

References 26 publications

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“…The antioxidant potential was evaluated by in vitro free radical scavenging activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, 36 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) method, 37 and ferric reducing antioxidant power assay (FRAP) method. 38 …”

Section: Methodsmentioning

confidence: 99%

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

et al. 2021

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The dietary triacylglycerol (TAG) gets absorbed and accumulated in the body through the monoacylglycerol (MAG) pathway, which plays a major role in obesity and related disorders. The main enzyme of this pathway, monoacylglycerol acyltransferase 2 (MGAT2), is considered as a potential target for developing antiobesity compounds. Hence, there is a need for in vitro cell-based assays for screening the potential leads for MGAT2 inhibitors. Because of synthetic inhibitor's side effects, there is an increased interest in natural extracts as potential leads. Hence, we have optimized a 2-MAG-induced TAG accumulation inhibitory cell-based assay to screen natural extracts using the HIEC-6 cell line. A concentration-dependent TAG accumulation was observed when the HIEC-6 cells were fed with exogenous 2-MAG. The TAG accumulation was confirmed by in situ BODIPY staining and was quantified. However, no TAG accumulation was seen when the cells were fed with exogenous DAG or TAG, suggesting MGAT2mediated MAG uptake and its conversion to TAG. We demonstrated the utility of this assay by screening five different plant-based aqueous extracts. These extracts showed various inhibition levels (25% to 30%) of 2-MAG-induced TAG accumulation in the HIEC-6. The MGAT2 inhibitory potential of these extracts was confirmed by an in vitro MGAT2 assay. This cell-based assay adds a new methodology for screening, developing, and evaluating MGAT2 inhibitors for addressing obesity and related disorders.

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Section: Methodsmentioning

confidence: 99%

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

et al. 2021

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“…The plant also possesses hepatoprotective, anti‐diabetic, α‐glucosidase inhibitory activity . The plant contains a significant amount of polyphenols (mostly phenolic acids, namely gallic acid, p ‐coumaric acid, ferulic acid, protocatechuic acid), and its glycosides, flavonoids (catechin, quercetin, anthocyanins) especially in its fruits . Various micronutrients, essential amino acids, triterpene saponins (acutosides), cucurbitacins and luffangulin have also been reported in the plant …”

Section: Introductionmentioning

confidence: 99%

“…14 The plant contains a significant amount of polyphenols (mostly phenolic acids, namely gallic acid, p-coumaric acid, ferulic acid, protocatechuic acid), and its glycosides, flavonoids (catechin, quercetin, anthocyanins) especially in its fruits. 15 Various micronutrients, essential amino acids, triterpene saponins (acutosides), cucurbitacins and luffangulin have also been reported in the plant. 11,16 The present study evaluated the extracts and partitioned fractions of L. acutangula for CA inhibition and total phenolic and flavonoid content; explored the mechanism of inhibition based on the reaction kinetics; tentatively identified phenolics in the most active fraction using ultra-performance liquid chromatography-quadrupole/time-offlight mass spectrometry (UPLC-QTOF-MS).…”

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confidence: 99%

UPLC‐QTOF‐MS analysis of a carbonic anhydrase‐inhibiting extract and fractions of Luffa acutangula (L.) Roxb (ridge gourd)

Chanda

Mukherjee

Biswas

et al. 2018

Phytochemical Analysis

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Introduction Luffa acutangula (L.) Roxb, commonly known as ridge gourd (cucurbitaceae), is a common vegetable cultivated in India. It is also a well‐used medicinal plant in Indian traditional medicine. Objectives To analyse the phenolics content of the most potent carbonic anhydrase‐inhibiting fraction from an extract of L. acutangula. Materials and Methods An aqueous ethanol extract of dried fruits of L. acutangula was successively fractionated into petroleum ether, dichloromethane and ethyl acetate. The extract and subsequent fractions were assessed for carbonic anhydrase‐inhibitory activity and the enzyme inhibition kinetics were determined for the most active fraction. Total phenolic and flavonoid content of the extract and subsequent fractions were determined spectrophotometrically. Ultra‐performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS) analysis was used to tentatively identify the major phenolics in the most active fraction. Results The concentration of total phenolics and total flavonoids in the extract and each fraction thereof correlated with the level of carbonic anhydrase inhibition activity. The ethyl acetate fraction of the aqueous ethanol extract of L. acutangula had the highest carbonic anhydrase inhibition activity. The enzyme kinetics analysis indicated a mixed mode of inhibition. UPLC‐QTOF‐MS analysis of the ethyl acetate fraction indicated a number of phenolic acids, hydroxycoumarins, flavones, flavanones, and flavonoids. Conclusion The correlation of total phenolic content with carbonic anhydrase inhibition suggested further research that might confirm that phenolic compounds of L. acutangula offer potential therapeutic benefits against carbonic anhydrase‐related disorders.

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“…Luffa acutangula (L.) and Luffa cylindrica (L.) are consumed because of the medicinal properties of their fruits [106,107]. The total phenolic and flavonoid contents in fruits of L. acutangula has been measured under methanol extraction as 24.6 mg GAE/g DW and 43.2 mg QE/g DW, respectively [108].…”

Section: Other Generamentioning

confidence: 99%

Genetic and Pre- and Postharvest Factors Influencing the Content of Antioxidants in Cucurbit Crops

2021

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Cucurbitaceae is one of the most economically important plant families, and includes some worldwide cultivated species like cucumber, melons, and squashes, and some regionally cultivated and feral species that contribute to the human diet. For centuries, cucurbits have been appreciated because of their nutritional value and, in traditional medicine, because of their ability to alleviate certain ailments. Several studies have demonstrated the remarkable contents of valuable compounds in cucurbits, including antioxidants such as polyphenols, flavonoids, and carotenoids, but also tannins and terpenoids, which are abundant. This antioxidant power is beneficial for human health, but also in facing plant diseases and abiotic stresses. This review brings together data on the antioxidant properties of cucurbit species, addressing the genetic and pre- and postharvest factors that regulate the antioxidant content in different plant organs. Environmental conditions, management, storage, and pre- and postharvest treatments influencing the biosynthesis and activity of antioxidants, together with the biodiversity of this family, are determinant in improving the antioxidant potential of this group of species. Plant breeding, as well as the development of innovative biotechnological approaches, is also leading to new possibilities for exploiting cucurbits as functional products.

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Characterization of nutrients, amino acids, polyphenols and antioxidant activity of Ridge gourd (Luffa acutangula) peel

Cited by 20 publications

References 26 publications

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

UPLC‐QTOF‐MS analysis of a carbonic anhydrase‐inhibiting extract and fractions of Luffa acutangula (L.) Roxb (ridge gourd)

Genetic and Pre- and Postharvest Factors Influencing the Content of Antioxidants in Cucurbit Crops

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