Characterization of nucleotides and nucleosides by pyrolysis-gas chromatography

The Application of Pyrolysis Techniques to the Identification of Micro‐organisms

“…No series of products characteristic of the class have been detected in nucleic acid pyrolysates. The carbohydrate moiety decomposes as expected to furans but the purine and pyrimidine bases undergo extensive degradation to hydrogen cyanide and a few organic compounds which are not definitive (Jennings & Dimick 1962;Turner 1969;Turner & Barr 1971). Certain pyrolysis products are specific for a particular compound or class of compound.…”

Differentiation of Salmonella Serotypes by pyrolysis-gas-liquid chromatography of cell fragments

“…Reiner and co-workers were the first to use PGLC for identification and taxonomic studies of bacteria (21)(22)(23)(24)26). This technique also was used to identify and characterize fungi (3,9,29,30,35), Vibrio cholerae (6), Clostridium botulinum types A, B, and E (4), streptococcal cell wall components (7), purines and pyrimidines (8), proteins, peptides, and amino acids (13,14), nucleotides and nucleosides (33), and ribonucleosides, ribonucleotides, and dinucleotides (34). PGLC techniques also could be useful in space exploration for detection of extraterrestrial life (19,31).…”