Characterization of Nuclear Polyhedrosis Viruses from Three Subspecies ofLambdina fiscellaria

Levin, David B.; Laitinen, Ann Marie; Clarke, Tom; Lucarotti, Christopher J.; Morin, Benoit; Otvos, I. S.

doi:10.1006/jipa.1996.4633

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…Previous investigations have also shown that the genomes of SpliNPV-B and AcMNPV are not closely related (Croizier et al 1989). Phylogenetic analyses based upon both polh (Levin et al 1997) and egt (Hu et al, 1997) suggest that SpliNPV-B is only distantly related to AcMNPV. Huang et al (1999) reported that SpliNPV-B isolate is an unusual NPV that appears distantly related to AcMNPV.…”

Section: Splinpv-tr1mentioning

confidence: 99%

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Ayhan;CENGİZ¹

2006

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Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nucleopolyhedrovirus (SpliNPV) is a member of Baculoviridae and belongs to the group II NPVs. Lef-8 is a well-conserved gene that is characterized in most of the lepidopteran NPVs. Nucleotide and aminoacid sequences of the encoded proteins and gene content may be phylogenetically informative. In this study, lef-8 gene in the Turkish SpliNPV-B (SpliNPV-TR1) genome was partially amplified by PCR and analyzed by amino-acid sequences with several baculoviruses. Amino-acid sequences of partial SpliNPV-TR1 lef-8 gene was 84 % identical to the lef-8 of SpltNPV while revealed it to have about only 57 %, 55 %, 51 %, 50 %, 50 %, 50 % and 49 % of its amino-acid sequence identity with AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV and BufuNPV, respectively. The sequences between SpliNPV-TR1 and granuloviruses also produced low levels of sequence identities (maximum 46 %). Although group II NPVs were rather isolated from noctuid hosts, the low level identity values detected between these noctuid NPVs (except the high idendity value between SpliNPV-TR1 and SpltNPV) revealed that these isolates can not have a monophyletic origin.Key Words: Baculovirus, spodoptera littoralis, noctuidae, spliNPV-TR1, lef-8, sequence, comparison. Türk SpliNPV-B İzolatından elde edilen lef-8 Geninin diğer Bakulovirüslerle Kısmi Sekans KarşılaştırmasıÖz: Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nukleopolihedrovirus (SpliNPV) Baculoviridae'nin bir üyesi olup grup II NPV'leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV'sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B (SpliNPV-TR1) genomundaki lef-8 geni PCR'la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir (en fazla % 46). Grup II NPV'leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV'leri arasında saptanan düşük dereceli benzerlikler (SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç), bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir.

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Section: Splinpv-tr1mentioning

confidence: 99%

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Ayhan;CENGİZ¹

2006

Tarım Bilimleri Dergisi

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“…2C). The probe cross-reacted with CfMNPV (87% identical [9]), moderately cross-reacted with LdNPV and LffNPVs (76% identical [25,27]), was minimally reactive to OpSNPV (76% identical [26]), and did not cross-react with NeabNPV (55% identical [11]). In contrast to PCR and Southern hybridization methods, the ELISA method was specific to O. pseudotsugata NPVs (Fig.…”

Section: Sensitivitymentioning

confidence: 99%

Development and Evaluation of Methods To Detect Nucleopolyhedroviruses in Larvae of the Douglas-Fir Tussock Moth, Orgyia pseudotsugata (McDunnough)

Thorne

Otvos

Conder

et al. 2007

Appl Environ Microbiol

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Various molecular methods are used to detect pathogenic microorganisms and viruses within their hosts, but these methods are rarely validated by direct comparison. Southern hybridization, enzyme-linked immunosorbent assay (ELISA), and a novel DNA extraction/PCR assay were used to detect Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV) in Douglas-fir tussock moth larvae. PCR was more sensitive than Southern hybridization and ELISA at detecting semipurified virus. ELISA, however, was the most accurate method for detecting virus within larvae, given that Southern hybridization and PCR produced false-negative results (31% and 2.5%, respectively). ELISA may be preferable in some applications because virus infections can be quantified (r 2 ‫؍‬ 0.995). These results may be applicable to both applied and academic research that seeks to accurately identify the incidence of viruses and microorganisms that regulate insect populations.

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“…NPVs have been isolated from all three subspecies (LafiNPV-W, LafiNPV-E, and LafiNPV-O, respectively;Morris 1964;Cunningham 1970b;Levin et al 1997), and bioassays have established that NPVs from each of the three produce similar levels of infection in L. fiscellaria fiscellaria larvae (Cunningham 1970a). Sohi and Cunningham (1972) demonstrated that the Malacosoma disstria cell line, Md108, is permissive for LafiNPV-O, and that OBs derived from LafiNPV-O-infected Md108 cells can infect L. fiscellaria fiscellaria larvae per os.…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of Lambdina fiscellaria lugubrosa nucleopolyhedrovirus in heterologous cell lines

Whittome-Waygood

Fraser

Lucarotti

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Infections of two heterologous insect cell lines derived from Malacosoma disstria (Md108) and Choristoneura fumiferana (Cf70) by the Lambdina fiscellaria lugubrosa nucleopolyhedrovirus (LafiNPV-W) were characterized. Cytopathic effects characteristic of LafiNPV-W infection, including rounding of cells, nuclear hypertrophy, and occlusion body (OB) production, were observed in both cell lines. Budded virus titers were slightly higher in Md108 cells than Cf70 cells (5.8 x 10(7) versus 3.1 x 10(7) TCID(50) units mL(-1)). Viral replication kinetics and cytopathic effects induced by LafiNPV-W infection were very similar in both cell lines. Actin rearrangements and redistribution of heterochromatin and euchromatin were observed within 24 h post-inoculation (hpi), and large quantities of nucleocapsids and virions were observed by electron microscopy at 48 hpi in both cell lines. Cf70 cultures produced OBs with numerous embedded virions, while OBs in Md108 cultures contained few virions or were empty with nucleocapsids packed in the nucleoplasm between OBs. In bioassays against second instar L. fiscellaria lugubrosa, OBs derived from LafiNPV-W-infected Md108 cells induced significantly lower levels of mortality than OBs derived from LafiNPV-W-infected Cf70 cells or from infected L. fiscellaria fiscellaria larvae.

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Characterization of Nuclear Polyhedrosis Viruses from Three Subspecies ofLambdina fiscellaria

Cited by 17 publications

References 14 publications

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Development and Evaluation of Methods To Detect Nucleopolyhedroviruses in Larvae of the Douglas-Fir Tussock Moth, Orgyia pseudotsugata (McDunnough)

In vitro culture of Lambdina fiscellaria lugubrosa nucleopolyhedrovirus in heterologous cell lines

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