Characterization of nuclear microsatellite loci in the Neotropical tree <i>Parkia panurensis</i> (Fabaceae)

Luettmann, Kathrin; Michalczyk, Inga M.; Mengel, Christina; Ziegenhagen, Birgit; Heymann, Eckhard W.; Saboya, Pedro Pablo Pinedo; Bialozyt, Ronald

doi:10.3732/ajb.1000096

Cited by 4 publications

(4 citation statements)

References 7 publications

(6 reference statements)

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“…We used nine highly polymorphic nuclear microsatellite loci for genotyping (Luettmann et al 2010). PCR and genotyping methods are reported in detail in Heymann et al (2012).…”

Section: Molecular Genetic Methods and Analyses Of Genetic Variationmentioning

confidence: 99%

Primate seed dispersal leaves spatial genetic imprint throughout subsequent life stages of the Neotropical tree Parkia panurensis

Bialozyt

Luettmann

Michalczyk

et al. 2014

Trees

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Key messageThe Neotropical tree Parkia panurensis shows a spatial genetic structure from the seed to the adult stage that is most likely the outcome of the seed dispersal provided by primates. Abstract Seed dispersal and pollination determine the gene flow within plant populations. In addition, seed dispersal creates the template for subsequent stages of plant recruitment. Therefore, the question arises whether and how seed dispersal affects the spatial genetic structure (SGS) of plant populations. In this study, we used microsatellites to analyse the SGS of the Neotropical tree Parkia panurensis (Fabaceae). This plant species is a major food resource for primates and its seeds are mainly dispersed by primates. Seeds were collected during behavioural observations of a tamarin mixed-species troop in northeastern Peru. Additionally, leaf samples of juveniles and of adults trees of this species were collected throughout the home range of the tamarin troop. A significant SGS for embryos (located within the dispersed seeds) and for nonreproductive plants are found up to a distance of 300 m. This matches the distance within which most seeds are dispersed. In the adult stage, the scale of a significant SGS is reduced to 100 m. While we cannot explain this scale reduction, our study provides the first evidence that primate seed dispersal does influence the SGS of a tropical tree species.

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“…We used nine highly polymorphic nuclear microsatellite loci for genotyping (Luettmann et al 2010). PCR and genotyping methods are reported in detail in Heymann et al (2012).…”

Section: Molecular Genetic Methods and Analyses Of Genetic Variationmentioning

confidence: 99%

Primate seed dispersal leaves spatial genetic imprint throughout subsequent life stages of the Neotropical tree Parkia panurensis

Bialozyt

Luettmann

Michalczyk

et al. 2014

Trees

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“…For the extraction of DNA, hexadecyltrimethylammonium bromide CTAB protocol was used as described by [21] and then quantified [17]. A total of 9 polymorphic and informative microsatellite primers developed for Parkia panurensis (Parpan 3, Parpan 4, Parpan 5, Parpan 9, Parpan11, Parpan13, Parpan14, Parpan15, and Parpan 21) (Table 1) were validated in heterologous amplification [22] and were used in the study. These microsatellite loci were amplified by polymerase chain reaction (PCR) using a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with a total volume of 10 µL per reaction (containing 10 ng of genomic DNA, 1× buffer, 210 µM of each dNTP, 1.5 mM MgCl 2 , 0.16 µM forward primer and M13 (FAM or NED) broth [23], 0.32 µM reverse primer, 1.05 U Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA), and 3.49 µL ultrapure water.…”

Section: Amplification Of Microsatellite Markersmentioning

confidence: 99%

Mating System Analysis and Genetic Diversity of Parkia multijuga Benth. One Native Tree Species of the Amazon

Oliveira,

Ramos,

Ferreira

et al. 2024

Forests

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The Amazonian native tree species Parkia multijuga has potential silvicultural characteristics that can be utilized to productive plantations. Understanding its mating system is necessary to delineate the methods for the breeding of the species, the collection of seeds for conservation, and the use of seedlings for production plantations. The aim of this study is to evaluate the mating system and population genetic diversity of P. multijuga, using molecular markers. The DNA of 221 plants was extracted and genotyped with nine microsatellite loci using capillary electrophoresis in an automated DNA sequencer. The estimates for single and multilocus crossing rates were 0.998 and 1.0, respectively. The paternity correlation was low (r^p(m) = 0.307). The fixation index (f) showed values below zero, indicating an excess of heterozygotes. The cluster number K = 2 shows a better grouping among families for genetic structure. P. multijuga families consist mainly of half-sibs, and the reproductive strategy of the species is allogamy.

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“…For DNA fingerprinting all seed coat and leaf samples were analysed at seven highly polymorphic nuclear microsatellite (nSSR) loci, previously characterised in Parkia panurensis : Parpan3, Parpan4, Parpan5, Parpan9, Parpan14, Parpan15, Parpan21 [40]. PCR amplifications were carried out in a Thermocycler (Biometra, Göttingen, Germany) using fluorescent labelled primers.…”

Section: Methodsmentioning

confidence: 99%

“…PCR amplifications were carried out in a Thermocycler (Biometra, Göttingen, Germany) using fluorescent labelled primers. PCR protocols are specified in [40]. Numbers of cycles were increased for the seed coats following the procedure with other exocarp material [41].…”

Section: Methodsmentioning

confidence: 99%

DNA Fingerprinting Validates Seed Dispersal Curves from Observational Studies in the Neotropical Legume Parkia

et al. 2012

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Background Determining the distances over which seeds are dispersed is a crucial component for examining spatial patterns of seed dispersal and their consequences for plant reproductive success and population structure. However, following the fate of individual seeds after removal from the source tree till deposition at a distant place is generally extremely difficult. Here we provide a comparison of observationally and genetically determined seed dispersal distances and dispersal curves in a Neotropical animal-plant system. Methodology/Principal Findings In a field study on the dispersal of seeds of three Parkia (Fabaceae) species by two Neotropical primate species, Saguinus fuscicollis and Saguinus mystax , in Peruvian Amazonia, we observationally determined dispersal distances. These dispersal distances were then validated through DNA fingerprinting, by matching DNA from the maternally derived seed coat to DNA from potential source trees. We found that dispersal distances are strongly right-skewed, and that distributions obtained through observational and genetic methods and fitted distributions do not differ significantly from each other. Conclusions/Significance Our study showed that seed dispersal distances can be reliably estimated through observational methods when a strict criterion for inclusion of seeds is observed. Furthermore, dispersal distances produced by the two primate species indicated that these primates fulfil one of the criteria for efficient seed dispersers. Finally, our study demonstrated that DNA extraction methods so far employed for temperate plant species can be successfully used for hard-seeded tropical plants.

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Characterization of nuclear microsatellite loci in the Neotropical tree Parkia panurensis (Fabaceae)

Abstract: All nine loci could also be verified in six other Parkia species and polymorphic fragments amplified. The new marker set can be used for future studies of genetic diversity and differentiation, as well as estimation of gene flow and parentage analyses in various Parkia species.

Cited by 4 publications

References 7 publications

Primate seed dispersal leaves spatial genetic imprint throughout subsequent life stages of the Neotropical tree Parkia panurensis

Primate seed dispersal leaves spatial genetic imprint throughout subsequent life stages of the Neotropical tree Parkia panurensis

Mating System Analysis and Genetic Diversity of Parkia multijuga Benth. One Native Tree Species of the Amazon

DNA Fingerprinting Validates Seed Dispersal Curves from Observational Studies in the Neotropical Legume Parkia

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