Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina

Consolo, Verónica Fabiana; Mónaco, Cecilia Inés; Cordo, Cristina Alicia; Salerno, Graciela L.

doi:10.1007/s11274-011-0938-5

Cited by 35 publications

(51 citation statements)

References 42 publications

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“…Plant Pathol., 9 (2): 46-58, 2015 rhizosphere soil (Harman et al, 2004). The variable in suppression capability of Trichoderma isolates obtained against soil-borne fungal plant pathogen (F. fujikuroi) was also reported by Consolo et al (2012). In dual culture plate testing, the mycelia of Trichoderma spp.…”

Section: Discussionmentioning

confidence: 93%

Potential of Trichoderma spp. as Biological Control Agents Against Bakanae Pathogen (Fusarium fujikuroi) in Rice

Ng¹,

Ngadin²,

azhari³

et al. 2015

Asian J. of Plant Pathology

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Bakanae disease is a major rice disease caused by Fusarium fujikuroi. Infected plants show slender and hyper elongated internodes due to the over-production of gibberellic acid. Application of Trichoderma spp. as biocontrol agent is gaining attention due to high capability in hyperparasitize the soil borne pathogen. The studies aimed to screen and evaluate the bio-efficacy of Trichoderma spp. with antagonistic activities against F. fujikuroi and plant growth-promoting properties. All the 65 Trichoderma isolates were isolated from healthy rice rhizosphere soil. Thirty eight out of 65 Trichoderma isolates exhibited more than 45 Percentage of Inhibition Radial Growth (PIRG) against F. fujikuroi in dual culture plate testing. All selected Trichoderma isolates were further in vitro screened for antagonistic testing: volatile compounds production and hydrogen cyanide production and plant growth-promotion properties: IAA production and phosphate solubilization. Twelve Trichoderma isolates were selected for further evaluation on antagonistic activity against F. fujikuroi, germination rate, plumule and radical lengths and vigor index. Finally, seven of the most potential Trichoderma isolates were selected for greenhouse evaluation. The bakanae disease incidence and disease severity in rice plant treated with respective selected Trichoderma isolates were significant reduced as compared with untreated plant. However, there was no significant increase in plant height between Trichoderma inoculated and uninoculated plants. Moreover, rice plant treated with Trichoderma T61 showed significantly increase in total plant dry biomass as compared to untreated plants. The selected Trichoderma isolates have potential to be developed as biological control agent against F. fujikuroi and also as an alternative for bakanae management.

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Section: Discussionmentioning

confidence: 93%

Potential of Trichoderma spp. as Biological Control Agents Against Bakanae Pathogen (Fusarium fujikuroi) in Rice

Ng¹,

Ngadin²,

azhari³

et al. 2015

Asian J. of Plant Pathology

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“…RAPD, ISSR and ITS markers proved to be useful as genetic markers in Trichoderma spp. fingerprinting [20,28,29]. Although major bands from these markers reactions are highly reproducible and potential confounding effects associated with co-migration with other markers.…”

Section: Cluster Analysis Of Trichoderma Parent's Strains and Their Fmentioning

confidence: 99%

Fingerprinting and molecular comparison among two parental strains of Trichoderma spp. and their corresponding fusants produced by protoplast fusion

Lakhani

Vakharia

Hassan

et al. 2016

Biotechnology & Biotechnological Equipment

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Protoplast fusions are important tools in strain improvement for bringing genetic recombination and developing hybrid strains in filamentous fungi. These are the powerful techniques for engineering of microbial strains with desirable different properties. The molecular characterization of two parent's strains (Trichoderma harzianum NBAII Th 1, Trichoderma viride NBAII Tv 23) and their corresponding fusants, produced by protoplast fusion, was investigated by random amplified polymorphic DNA (RAPD), ISSR (inter-simple sequence repeats) and internal transcribed spacer (ITS) markers. Specific results for fingerprinting were obtained by the 20 primers of each RAPD and ISSR, and four primers of ITS, respectively. These markers produced different fragment patterns with varied number of bands and yielded a total of 419 distinct bands. 13.3% were considered as polymorphic bands and 86.7% were considered as monomorphic bands. For RAPD marker, the OPC-04 primer has showed the highest polymorphism, 58.3%. While in ISSR marker, the UBC-835 primer has showed the highest polymorphism, 57.2%. The dendrogram based on RAPD marker results grouped the two parent's strains and 21 fusants into two different clusters with about 97% genetic similarity. While the dendrogram based on ISSR and ITS markers results grouped the two parent's strains and 21 fusants into two different clusters with about 88% genetic similarity. Moreover, the smallest genetic distance (0.01) was estimated between fusant-10 and fusant-12; moreover, fusant-8 and fusant-20 relatively have the highest genetic distance. Our results are consistent with the conclusion that understanding the genetic variation within parent Trichoderma strains and their corresponding fusants and molecular occurrence of protoplast fusion using RAPD, ISSR and ITS markers.

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“…This made it possible to search for all scaffolds in one go. Screening the genome for microsatellite motifs was based on published data about existing motifs that were detected in T. virens by ISSR and RAMS analysis [31,32]. Microsatellite loci sufficiently flanked by unique sequences were selected for primer design using the Primer3 software [33] with parameter settings that included the GC-clamp option and an allowance for primer annealing at approximately 60 °C.…”

Section: Screening Of Databases and Primer Designmentioning

confidence: 99%

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

et al. 2015

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Using biological fertilizers and pesticides based on beneficial soil microbes in order to reduce mineral fertilizers and chemical pesticides in conventional agriculture is still a matter of debate. In this regard, a European research project seeks to elucidate the role of root-endophytic fungi and to develop molecular tools to trace and quantify these fungi in the rhizosphere and root tissue. To do this, the draft genome sequence of the biocontrol fungus Trichoderma virens (T. virens) was screened for simple sequence repeats (SSRs) and primers were developed for 12 distinct loci. Primers were evaluated using a global collection of ten isolates where an average of 7.42 alleles per locus was detected. Nei's standard genetic distance ranged from 0.18 to 0.27 among the isolates, and the grand mean of haploid diversity in AMOVA analysis was 0.693 ± 0.019. Roots of tomato plants were inoculated with different strains and harvested six weeks later. Subsequent PCR amplification identified root-endophytic strains and co-colonization of roots by different strains. Markers were applied to qPCR to quantify T. virens strains in root tissue and to determine their identity using allele-specific melting curve analysis. Thus, the root-endophytic lifestyle of T. virens was OPEN ACCESSDiversity 2015, 7 361 confirmed, strains in roots were quantified and simultaneous colonization of roots by different strains was observed.

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Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina

Cited by 35 publications

References 42 publications

Potential of Trichoderma spp. as Biological Control Agents Against Bakanae Pathogen (Fusarium fujikuroi) in Rice

Potential of Trichoderma spp. as Biological Control Agents Against Bakanae Pathogen (Fusarium fujikuroi) in Rice

Fingerprinting and molecular comparison among two parental strains of Trichoderma spp. and their corresponding fusants produced by protoplast fusion

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

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