Characterization of novel heat-responsive transcription factor (TaHSFA6e) gene involved in regulation of heat shock proteins (HSPs) — A key member of heat stress-tolerance network of wheat

Kumar, Ranjeet; Goswami, Suneha; Singh, Khushboo; Dubey, Kavita; Kumar, Girjesh; Singh, Bhupinder; Singh, S. P.; Grover, Monendra; Mishra, Dwijesh Chandra; Kumar, Sanjeev; Bakshi, Suman; Rai, Anil; Pathak, Himanshu; Chinnusamy, Viswanathan; Praveen, Shelly

doi:10.1016/j.jbiotec.2018.05.008

Cited by 46 publications

(24 citation statements)

References 55 publications

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“…Members of the HSF family have been identified in wheat based on the old reference genome [32], but a lack of sufficient information has hindered in-depth studies of the stress resistance mechanisms related to TaHsfs. A limited number of TaHsfs have been functionally analyzed, and studies have demonstrated that TaHsfA4a, TaHsfA6e, TaHsfB2d, and TaHsfC2a function as wheat HSF genes to enhance the tolerance of heat, confer strong cadmium tolerance, and participate in protecting the grains [28,[33][34][35]. Therefore, a more comprehensive and systematic analysis is required of TaHsf s based on the newly published wheat reference genome in order to provide a reliable basis for elucidating their functions.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development

Yang

Gan

et al. 2020

IJMS

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Heat shock transcription factors (HSFs) play crucial roles in resisting heat stress and regulating plant development. Recently, HSFs have been shown to play roles in anther development. Thus, investigating the HSF family members and identifying their protective roles in anthers are essential for the further development of male sterile wheat breeding. In the present study, 61 wheat HSF genes (TaHsfs) were identified in the whole wheat genome and they are unequally distributed on 21 chromosomes. According to gene structure and phylogenetic analyses, the 61 TaHsfs were classified into three categories and 12 subclasses. Genome-wide duplication was identified as the main source of the expansion of the wheat HSF gene family based on 14 pairs of homeologous triplets, whereas only a very small number of TaHsfs were derived by segmental duplication and tandem duplication. Heat shock protein 90 (HSP90), HSP70, and another class of chaperone protein called htpG were identified as proteins that interact with wheat HSFs. RNA-seq analysis indicated that TaHsfs have obvious period- and tissue-specific expression patterns, and the TaHsfs in classes A and B respond to heat shock, whereas the C class TaHsfs are involved in drought regulation. qRT-PCR identified three TaHsfA2bs with differential expression in sterile and fertile anthers, and they may be candidate genes involved in anther development. This comprehensive analysis provides novel insights into TaHsfs, and it will be useful for understanding the mechanism of plant fertility conversion.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development

Yang

Gan

et al. 2020

IJMS

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“…Additionally, AtHsfA6b was reported to be involved in ABA signaling and required for ABA-mediated heat stress resistance [15]. Several Hsfs in other plant species, including tomato SlHsfA1 [16], tall fescue FaHsfA2c [17], wheat TaHsfA6e [18], and maize ZmHsf05 [19], have also been proved to participate in thermotolerance.…”

Section: Introductionmentioning

confidence: 99%

Wheat Heat Shock Factor TaHsfA6f Increases ABA Levels and Enhances Tolerance to Multiple Abiotic Stresses in Transgenic Plants

Zhao

et al. 2020

IJMS

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Abiotic stresses are major constraints limiting crop growth and production. Heat shock factors (Hsfs) play significant roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. In this study, we explored the biological functions and underlying mechanisms of wheat TaHsfA6f in plant tolerance to various abiotic stresses. Gene expression profiles showed that TaHsfA6f has relatively high expression levels in wheat leaves at the reproductive stage. Transcript levels of TaHsfA6f were substantially up-regulated by heat, dehydration, salinity, low temperature, and multiple phytohormones, but was not induced by brassinosteroids (BR). Subcellular localization analyses revealed that TaHsfA6f is localized to the nucleus. Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA. Furthermore, RNA-sequencing data demonstrated that TaHsfA6f functions through up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genes. Collectively, these results provide evidence that TaHsfA6f participates in the regulation of multiple abiotic stresses, and that TaHsfA6f could serve as a valuable gene for genetic modification of crop abiotic stress tolerance.

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“…Similarly, HSFs have been reported to play a key role in plant acclimation to abiotic stress conditions. Kumar et al reported that TaHSFA6e modulates tolerance of wheat to HS and drought stress during pollination and grain filling stages [98]. Yokotani et al reported that OsHSFA2e improves Arabidopsis tolerance to HS and salinity stress by activating the expression of HSPs [99].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Investigation and Expression Profiles of Heat Shock Transcription Factor (HSF) Gene Family in Maize (<em>Zea mays</em> L.) under Different Growth Stages and Abiotic Stress Conditions

Haider¹,

Rehman²,

Ahmad³

et al. 2021

Preprint

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Heat shock transcription factors (HSFs) participate in regulating many environmental stress responses and biological processes in plants. Maize (Zea mays L.) is a major cash crop that is grown worldwide. However, the growth and yield of maize are affected by several adverse environmental inputs. Therefore, investigating the factors that regulate maize growth and development and resistance to abiotic stress is an essential task for developing stress-resilient maize varieties. Thus, a comprehensive genome-wide identification analysis was performed to identify HSFs in the maize genome. The current study identified 25 ZmHSFs, randomly distributed throughout the maize genome. Phylogenetic analysis revealed that ZmHSFs are divided into three classes and 13 sub-classes. Gene structure and protein motif analysis supported the results obtained through the phylogenetic analysis. Domain analysis showed the DNA-binding domain to be the most conserved region of ZmHSFs. Segmental duplication is shown to be responsible for the expansion of ZmHSFs. Most of the ZmHSFs are localized inside the nucleus, and the ZmHSFs which belong to the same group show similar physio-chemical properties. The 3D structures revealed comparable conserved ZmHSFs protein structures. RNA-seq analysis revealed a major role of class A HSFs including, ZmHSFA-1a and ZmHSFA-2a in all the maize growth stages, i.e., seed, vegetative, and reproductive development. Furthermore, ZmHSFs displayed an obvious spatiotemporal expression. Under abiotic stress conditions (heat, drought, cold, UV, and salinity), members of class A and B ZmHSFs are induced. Gene ontology (GO) annotation analysis indicated a major role of ZmHSFs in resistance to environmental stress and regulation of primary metabolism. Further, the protein-protein interaction analysis showed that ZmHSFs interact with several molecular chaperons and major stress-responsive proteins. To summarize, this study provides novel insights for functional studies on the ZmHSFs in maize breeding programs.

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Characterization of novel heat-responsive transcription factor (TaHSFA6e) gene involved in regulation of heat shock proteins (HSPs) — A key member of heat stress-tolerance network of wheat

Cited by 46 publications

References 55 publications

Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development

Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development

Wheat Heat Shock Factor TaHsfA6f Increases ABA Levels and Enhances Tolerance to Multiple Abiotic Stresses in Transgenic Plants

Genome-wide Investigation and Expression Profiles of Heat Shock Transcription Factor (HSF) Gene Family in Maize (<em>Zea mays</em> L.) under Different Growth Stages and Abiotic Stress Conditions

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