Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis

Eid, André; Torres-Padilla, Maria-Elena

doi:10.1080/15592294.2016.1172160

Cited by 13 publications

(9 citation statements)

References 42 publications

(66 reference statements)

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“…In addition, PRC1.6 is the only PRC1 complex that possesses histone deacetylation activity46,47, which may also impact on the global histone hyperaceylation observed in 2-cell-like-cells24,27. Notably, specific components of PRC1.6, such as L3MBTL2 and RYBP, are low or absent from embryonic chromatin in 2-cell-stage embryos58. While work on induced pluripotency indicates that reprogramming does not necessarily recapitulate developmental progression in reverse order, investigating whether the factors that we identified are responsible for regulating the 2-cell transition in vivo will be an important task for the future.…”

Section: Discussionmentioning

confidence: 99%

A molecular roadmap for the emergence of early-embryonic-like cells in culture

et al. 2017

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Unlike pluripotent cells, which generate only embryonic tissues, totipotent cells can generate a full organism, including extraembryonic annexes. A rare population of cells resembling 2-cell stage embryos arises in pluripotent embryonic stem (ES) cell cultures. These 2-cell-like-cells display molecular features of totipotency and broader developmental plasticity. However, their specific nature and the process through which they arise remain outstanding questions. Here, we identify intermediate cellular states and molecular determinants during the emergence of 2-cell-like-cells. By deploying a quantitative single cell expression approach, we identified an intermediate population characterised by the expression of the transcription factor ZSCAN4 as precursor of 2-cell-like-cells. Using an siRNA screening, we uncovered novel epigenetic regulators of 2-cell-like-cell emergence, including the non-canonical PRC1 complex PRC1.6 and Ep400/Tip60. Our data shed light on the mechanisms underlying the exit from the ES cell state towards the formation of early-embryonic-like cells in culture and identify key epigenetic pathways that promote this transition.

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Section: Discussionmentioning

confidence: 99%

A molecular roadmap for the emergence of early-embryonic-like cells in culture

et al. 2017

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“…The maternally inherited YAF2 is often localized in the pericentromeric regions of the zygote. RYBP is absent in this stage but strongly induced from the 4-cell stage onward, while YAF2 protein is present only in these early stages but becomes almost undetectable after the 4-cell stage in mouse embryos [136]. Rybp is an essential gene, Rybp -/- mouse embryos die soon after implantation (E5.5–E6) [137], proving that Rybp has an essential role during early embryonic development, which cannot be compensated by the presence of Yaf2 .…”

Section: Connections Between Rybp and Germ Cell Fate Regulationmentioning

confidence: 99%

Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription

Bajusz

Henry

Sutus

et al. 2019

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Separation of germline cells from somatic lineages is one of the earliest decisions of embryogenesis. Genes expressed in germline cells include apoptotic and meiotic factors, which are not transcribed in the soma normally, but a number of testis-specific genes are active in numerous cancer types. During germ cell development, germ-cell-specific genes can be regulated by specific transcription factors, retinoic acid signaling and multimeric protein complexes. Non-canonical polycomb repressive complexes, like ncPRC1.6, play a critical role in the regulation of the activity of germ-cell-specific genes. RING1 and YY1 binding protein (RYBP) is one of the core members of the ncPRC1.6. Surprisingly, the role of Rybp in germ cell differentiation has not been defined yet. This review is focusing on the possible role of Rybp in this process. By analyzing whole-genome transcriptome alterations of the Rybp-/- embryonic stem (ES) cells and correlating this data with experimentally identified binding sites of ncPRC1.6 subunits and retinoic acid receptors in ES cells, we propose a model how germ-cell-specific transcription can be governed by an RYBP centered regulatory network, underlining the possible role of RYBP in germ cell differentiation and tumorigenesis.

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“…The revealed biological functions of L3MBTL2 are still limited. L3MBTL2 has been shown to have an essential role in early embryonic development and pluripotent stem cells ( Qin et al., 2012 ; Eid and Torres-Padilla, 2016 ). L3MBTL2 is highly expressed in testicular tissues and is involved in chromatin remodeling throughout meiosis and spermatogenesis ( Meng et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%