Characterization of NF–Y gene family and their expression and interaction analysis in Phalaenopsis orchid

Jiang, Li; Ren, Yuepeng; Jiang, Yifan; Hu, Shasha; Wu, Jiayi; Wang, Guangdong

doi:10.1016/j.plaphy.2023.108143

Cited by 1 publication

(2 citation statements)

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“…In Phalaenopsis, the PhNF-YC7 gene and other NF-Y family genes may indirectly regulate plant development and flowering time in response to low temperatures. We speculate that the NF-YC subfamily in Cymbidium may also have similar functional responses [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…The PhNF-Y gene family—especially the PhNF-YA and PhNF-YB subfamilies—exhibited a comprehensive response to cold, heat, drought, and salt stress [ 18 ]. Recent studies have investigated the NF-Y gene family in corn [ 19 ], Chinese cabbage [ 20 ], melon [ 21 ], peach [ 2 ], and Phalaenopsis orchid [ 22 ], demonstrating their significance in physiological ecology and abiotic stress responses across a variety of species.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Drought Stress Response Pattern of the NF-Y Gene Family in Cymbidium sinense

Wang,

Zhao,

Zheng

et al. 2024

IJMS

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Cymbidium sinense, a type of orchid plant, is more drought-resistant and ornamental than other terrestrial orchids. Research has shown that many members of the NUCLEAR FACTOR Y (NF-Y) transcription factor family are responsive to plant growth, development, and abiotic stress. However, the mechanism of the NF-Y gene family’s response to abiotic stress in orchids has not yet been reported. In this study, phylogenetic analysis allowed for 27 CsNF-Y genes to be identified (5 CsNF-YAs, 9 CsNF-YBs, and 13 CsNF-YC subunits), and the CsNF-Ys were homologous to those in Arabidopsis and Oryza. Protein structure analysis revealed that different subfamilies contained different motifs, but all of them contained Motif 2. Secondary and tertiary protein structure analysis indicated that the CsNF-YB and CsNF-YC subfamilies had a high content of alpha helix structures. Cis-element analysis showed that elements related to drought stress were mainly concentrated in the CsNF-YB and CsNF-YC subfamilies, with CsNF-YB3 and CsNF-YC12 having the highest content. The results of a transcriptome analysis showed that there was a trend of downregulation of almost all CsNF-Ys in leaves under drought stress, while in roots, most members of the CsNF-YB subfamily showed a trend of upregulation. Additionally, seven genes were selected for real-time reverse transcription quantitative PCR (qRT-PCR) experiments. The results were generally consistent with those of the transcriptome analysis. The regulatory roles of CsNF-YB 1, 2, and 4 were particularly evident in the roots. The findings of our study may make a great contribution to the understanding of the role of CsNF-Ys in stress-related metabolic processes.

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Section: Discussionmentioning

confidence: 99%