Characterization of new yeast isolates collected from different fruits in Tunisia and biocontrol activity against Penicillium expansum on apples

Kheireddine, A.; Palmieri, Davide; Vitullo, D.; Barberio, Antonia; Zouaoui, M.; Curtis, Filippo De; Sadfi‐Zouaoui, Najla; Lima, Giuseppe

doi:10.1007/s42161-021-00921-2

Cited by 6 publications

(1 citation statement)

References 70 publications

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“…The microorganisms used in this study were the mycotoxigenic postharvest pathogen P. expansum strain 7015, deposited in the “Toxigenic Fungi Culture Collection” of the Institute of Sciences of Food Production of the National Research Council (ISPA CNR—Bari, Italy), and the basidiomycetous yeast P. terrestris strain LS28, already known as a post-harvest biocontrol agent ( Lima et al, 1999 ; Kheireddine et al, 2021 ). The fungal inoculum was prepared by culturing P. expansum on Potato Dextrose Agar medium (PDA) for 10 days at 25°C in the dark.…”

Section: Methodsmentioning

confidence: 99%

Modulation of extracellular Penicillium expansum-driven acidification by Papiliotrema terrestris affects biosynthesis of patulin and has a possible role in biocontrol activity

et al. 2022

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The active regulation of extracellular pH is critical for the virulence of fungal pathogens. Penicillium expansum is the causal agent of green-blue mold on stored pome fruits and during its infection process acidifies the host tissues by secreting organic acids. P. expansum is also the main producer of patulin (PAT), a mycotoxin found in pome fruit-based products and that represents a serious health hazard for its potential carcinogenicity. While it is known that PAT biosynthesis in P. expansum is regulated by nutritional factors such as carbon and nitrogen and by the pH, the mechanistic effects of biocontrol on PAT production by P. expansum are not known. In this work, we assessed how optimal and suboptimal concentrations of the biocontrol agent (BCA) Papiliotrema terrestris LS28 affect both extracellular pH and PAT biosynthesis in P. expansum. In wounded apples, the optimal and suboptimal concentrations of the BCA provided almost complete and partial protection from P. expansum infection, respectively, and reduced PAT contamination in both cases. However, the suboptimal concentration of the BCA increased the specific mycotoxigenic activity by P. expansum. In vitro, the rate of PAT biosynthesis was strictly related to the extracellular pH, with the highest amount of PAT detected in the pH range 4–7, whereas only traces were detectable at pH 3. Moreover, both in vitro and in apple wounds the BCA counteracted the extracellular P. expansum-driven acidification maintaining extracellular pH around 4, which is within the pH range that is optimal for PAT biosynthesis. Conversely, in the absence of LS28 the pathogen-driven acidification led to rapidly achieving acidic pH values (<3) that lie outside of the optimal pH range for PAT biosynthesis. Taken together, these results suggest that pH modulation by LS28 is important to counteract the host tissue acidification and, therefore, the virulence of P. expansum. On the other hand, the buffering of P. expansum-driven acidification provided by the BCA increases the specific rate of PAT biosynthesis through the extension of the time interval at which the pH value lies within the optimal range for PAT biosynthesis. Nevertheless, the antagonistic effect provided by the BCA greatly reduced the total amount of PAT.

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Section: Methodsmentioning

confidence: 99%

Modulation of extracellular Penicillium expansum-driven acidification by Papiliotrema terrestris affects biosynthesis of patulin and has a possible role in biocontrol activity

et al. 2022

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Biocontrol activities of grey mould of grapes with the volatile organic compounds generated by yeast HXMG-1 isolated from grapes

Li,

Wang,

Cui

et al. 2024

J Plant Dis Prot

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The pathogenic bacterias of postharvest crops are reduced by volatile organic compounds (VOCs) generated by yeasts. The yeast HXMG-1, which works well against gray mold of grapes pathogens (Botrytis cinerea), was assessed for its potential to create volatile organic compounds (VOCs) as one of its ways of working. Using a double Petri dish assay, the effect of HXGM-1-produced VOCs on mycelial development and spore development of the target pathogens was evaluated. Compared to the control, the VOCs produced by yeast HXMG-1 signi cantly restricted the growth of mycelium and spore germination of Botrytis cinerea. Speci cally, the mycelium growth of Botrytis cinerea was entirely restricted and the rate of spore germination of Botrytis cinerea only 20.11% at a concentration of 1×10 9 CFU/mL. It was also found that the VOCs could signi cantly inhibit mycelium growth with 82.46% inhibition with treatment of concentration of 1×10 8 CFU/mL. The VOCs caused the mycelium to grow curved, resulting in larger mycelial tips, fewer nuclei, and shorter mycelial septum spacing. In in vivo tests, noninjure or injure grapes were arti cially inoculated with pathogen hyphal disk followed by biofumigation with VOCs generated by yeast HXMG-1, and the treatments (Wp2 and Wp3) signi cantly controlled pathogenic infection, con rming the results of in vitro tests. Through the creation of a phylogenetic tree, HXMG-1 was recognized as a member of the Ascomycota, Hemiascomycota, Yeasts, and Hansenula sp. families. In conclusion, the yeast strain HXMG-1 created VOCs that signi cantly inhibited the development of Botrytis cinerea on grapes and is expected to be further developed and utilized. This study lays the foundation for the using of Hansenula sp. for biological control of postharvest diseases.

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Copper sulphate inhibits Penicillium olsonii growth and conidiogenesis on Cannabis sativa

et al. 2023

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Characterization of new yeast isolates collected from different fruits in Tunisia and biocontrol activity against Penicillium expansum on apples

Cited by 6 publications

References 70 publications

Modulation of extracellular Penicillium expansum-driven acidification by Papiliotrema terrestris affects biosynthesis of patulin and has a possible role in biocontrol activity

Modulation of extracellular Penicillium expansum-driven acidification by Papiliotrema terrestris affects biosynthesis of patulin and has a possible role in biocontrol activity

Biocontrol activities of grey mould of grapes with the volatile organic compounds generated by yeast HXMG-1 isolated from grapes

Copper sulphate inhibits Penicillium olsonii growth and conidiogenesis on Cannabis sativa

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