Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate

Castellane, Tereza Cristina Luque; Campanharo, João Carlos; Colnago, Luiz Alberto; Coutinho, Isabel; Lopes, Érica Mendes; Lemos, Manoel Victor Franco; Lemos, Eliana Gertrudes de Macedo

doi:10.1016/j.ijbiomac.2016.11.123

Cited by 39 publications

(13 citation statements)

References 33 publications

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“…The present study indicated that the emulsifying activity of EPS extracts was dependent on the type of substrate used for bacterial growth, which is consistent with other reports, wherein Rhizobium tropici grew on hydrocarbon-based substrates and marine bacteria employed substrate-speci c hydrocarbon metabolism (SureshKumar et al 2007;Castellane et al 2017). The criterion for determining the emulsion-stabilizing ability of an emulsi er is to evaluate its ability to maintain at least 50% of the original volume of the emulsion for 24 h (Willumsen and Karlson 1997; Camacho-Chab et al 2013).…”

supporting

confidence: 92%

Differences In The Properties of Extracellular Polymeric Substances Responsible For PAH Degradation Isolated From Mycobacterium Gilvum SN12 Grown On Pyrene And Benzo[a]pyrenee

Jia

Liu

Gong

et al. 2021

Preprint

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To evaluate the differences in the characteristics of extracellular polymeric substances (EPS) secreted by Mycobacterium gilvum SN12 (M.g. SN12) cultured on pyrene (Pyr) and benzo[a]pyrene (BaP) , heating method was used to extract EPS from M.g. SN12, and the composition, emulsifying activity and morphology of EPS extracts were investigated in this study. It was indicated that EPS extracts varied signiﬁcantly with the addition of Pyr or BaP to the bacterial cultures. Concentration of proteins and carbohydrates, the main components of the EPS extracts, first increased and then decreased, with an increase in the concentration of Pyr (0–120 mg L-1) and BaP (0–120 mg L-1). A similar trend was observed for the emulsifying activity of the EPS extracts. EPS extracted from all cultures exhibited a compact structure with smooth surface, except EPS extracted from BaP-grown M.g. SN12, which exhibited a more fragile and softer surface. These findings suggest that Pyr and BaP had different influence on the properties of isolated EPS, providing insights into the mechanism underlying PAH biodegradation by some bacteria secreting EPS. To the best of our knowledge, this is the first report on the texture profile of EPS samples extracted from M.g. SN12 grown on PAHs.

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supporting

confidence: 92%

Differences In The Properties of Extracellular Polymeric Substances Responsible For PAH Degradation Isolated From Mycobacterium Gilvum SN12 Grown On Pyrene And Benzo[a]pyrenee

Jia

Liu

Gong

et al. 2021

Preprint

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“…The high intensity peak at around 1030 cm –1 indicated that the EPS sample contained pyranose, while the peaks at 982 cm –1 and 890 cm –1 referred to the absorption of d -glucopyranosyl and α- d -mannopyranose, respectively. Thus, since the presence of the above found vibrational modes in the low frequencies region of the FTIR spectrum, it can be concluded that this range effectively represented the polysaccharide characteristics of EPS samples [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Polysaccharide Based Polymers Produced by Scabby Cankered Cactus Pear (Opuntia ficus-indica L.) Infected by Neofusicoccum batangarum: Composition, Structure, and Chemico-Physical Properties

Santagata

Cimmino²,

Poggetto

et al. 2022

Biomolecules

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Neofusiccocum batangarum is the causal agent of scabby canker of cactus pear (Opuntia ficus-indica L.). The symptoms of this disease are characterized by crusty, perennial cankers, with a leathery, brown halo. Characteristically, a viscous polysaccharide exudate, caking on contact with air, leaks from cankers and forms strips or cerebriform masses on the surface of cactus pear cladodes. When this polysaccharide mass was partial purified, surprisingly, generated a gel. The TLC analysis and the HPLC profile of methyl 2-(polyhydroxyalkyl)-3-(o-tolylthiocarbomoyl)-thiazolidine-4R-carboxylates obtained from the mixture of monosaccharides produced by acid hydrolysis of the three EPSs examined in this research work [the polysaccharide component of the exudate (EPSC) and the EPSs extracted from asymptomatic (EPSH) and symptomatic (EPSD) cladodes] showed the presence of d-galactose, l-rhamnose, and d-glucose in a 1:1:0.5 ratio in EPSC while d-galactose, l-rhamnose, d-glucose, and d-xylose at the same ratio were observed in EPSH and EPSD. The presence of uronic acid residues in EPSC was also showed by solid state NMR and IR investigation. Furthermore, this manuscript reports the chemical-physical characterization of the gel produced by the infected cactus pear.

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“…The EPS pellet was precipitated by centrifugation and dried at 30°C for a day. The EPS was measured as dry weight (mg) per 100 ml culture ( Castellane et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions

Wongdee

Yuttavanichakul

Longthonglang

et al. 2021

Polish Journal of Microbiology

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The development of rhizobial inoculants with increased resistance to abiotic stress is critical to mitigating the challenges related to climate change. This study aims at developing a soybean stress-tolerant Bradyrhizobium inoculant to be used under the mixed stress conditions of acidity, high temperature, and drought. Six isolates of Bradyrhizobium with high symbiotic performance on soybean were tested to determine their growth or survival abilities under in vitro conditions. The representative stress-tolerant Bradyrhizobium isolates 184, 188, and 194 were selected to test their ability to promote soybean growth under stress conditions compared to the type strain Bradyrhizobium diazoefficiens USDA110. The plant experiment indicated that isolate 194 performed better in symbiosis with soybean than other Bradyrhizobium strains under stress conditions. Based on the stress tolerance index, soybeans inoculated with isolate 194 showed a high growth performance and significantly better nodulation competition ability than USDA110 under several stress conditions. Interestingly, supplementation of sucrose in the culture medium significantly enhances the survival of the isolate and leads to improved plant biomass under various stress conditions. Analysis of the intra-cellular sugars of isolate 194 supplemented with sucrose showed the accumulation of compatible solutes, such as trehalose and glycerol, that may act as osmoprotectants. This study indicates that inoculation of stress-tolerant Bradyrhizobium together with sucrose supplementation in a medium could enhance bacterial survival and symbiosis efficiency under stress conditions. Although it can be applied for inoculant production, this strategy requires validation of its performance in field conditions before adopting this technology.

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Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate

Cited by 39 publications

References 33 publications

Differences In The Properties of Extracellular Polymeric Substances Responsible For PAH Degradation Isolated From Mycobacterium Gilvum SN12 Grown On Pyrene And Benzo[a]pyrenee

Differences In The Properties of Extracellular Polymeric Substances Responsible For PAH Degradation Isolated From Mycobacterium Gilvum SN12 Grown On Pyrene And Benzo[a]pyrenee

Polysaccharide Based Polymers Produced by Scabby Cankered Cactus Pear (Opuntia ficus-indica L.) Infected by Neofusicoccum batangarum: Composition, Structure, and Chemico-Physical Properties

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions

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