Characterization of neuronal regeneration in the abdominal ganglion ofAplysia californica

Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characterized two such mRNAs following axotomy. One encodes a novel fasciclin-I homologue, Aplysia fasciclin-like protein (apFasP), and the other encodes Aplysia beta-thymosin (apbetaT). In addition to mRNA synthesis, proteins required for regeneration must be available at the site of growth, and the transport and local translation of certain extrasomatic mRNAs aids in this process. We found apbetaT and apFasP proteins and mRNA at growth cones in vitro. However, only the mRNA for apbetaT was present in regenerating axons in vivo. This implies that the membrane protein apFasP is supplied by rapid transport from the soma, whereas the soluble apbetaT is synthesized locally.

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Endogenous neurotrophic factors enhance neurite growth by bag cell neurons of Aplysia

Vanmali

Romanova

Messner

et al. 2003

J. Neurobiol.

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Mechanisms that regulate neurite outgrowth are phylogenetically conserved, including the signaling molecules involved. Here, we describe neurotrophic effects on isolated bag cell neurons (BCNs) of substrate-bound growth factors endogenous to the sea slug Aplysia californica. Sheath cells dissociated from the pleural-visceral connectives of the Aplysia CNS and arterial cells dissociated from the anterior aorta enhance neurite outgrowth when compared to controls, i.e., BCNs grown in defined medium alone. In addition, the substrate remaining after sheath cells or arterial cells are killed significantly enhances growth, relative to all other conditions tested. For instance, primary neurites are more numerous and greater in length for BCNs cultured on substrate produced by arterial cells. These results suggest that sheath and arterial cells produce growth-promoting factors, some of which are found in the substrates produced by these cell types. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), we found that Aplysia collagen-like peptides are produced by dissociated arterial cells, and therefore likely contribute to the observed growth effects. Collagen-like peptides and other factors produced by sheath and arterial cells likely influence neurite growth in the Aplysia CNS during development, learning and memory, and regeneration after injury.

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Characterization of neuronal regeneration in the abdominal ganglion ofAplysia californica

Cited by 8 publications

References 48 publications

Regeneration as an application of gastropod neural plasticity

Regeneration as an application of gastropod neural plasticity

mRNAs encoding theAplysia homologues of fasciclin-I and β-thymosin are expressed only in the second phase of nerve injury and are differentially segregated in axons regenerating in vitro and in vivo

Endogenous neurotrophic factors enhance neurite growth by bag cell neurons of Aplysia

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