Characterization of native fluorescent Pseudomonas isolates associated with alfalfa roots in Uruguayan agroecosystems

Characterization of the antifungal and plant growth-promoting effects of diffusible and volatile organic compounds produced by Pseudomonas fluorescens strains

“…Current efforts to isolate, characterize and select the best bacterial antagonists to control phytopathogens worldwide are continuously reported in the literature (Yanes et al, 2012;Guiñazú et al, 2013;Mavrodi et al, 2012;Rashid et al, 2012), though very few studies have demonstrated the presence of direct or indirect mechanisms in the reported antagonists. Therefore, in this study, four novel rhizospheric strains belonging to the P. fluorescens species were isolated and characterized for their significant antagonism toward the gray mould phytopathogen B. cinerea.…”

“…Therefore, the biological control of fungal diseases by microbial agents appears to be an excellent option, as secondary damaging effects on the environment can be null or minimal, in addition to the advantage of being able to export products abroad without restriction (versus the use of chemicals). Fortunately, efforts to isolate and characterize endogenous biocontrol agents are ongoing in many research labs world-wide (Selin et al, 2010;Yanes et al, 2012;Guiñazú et al, 2013;Lagzian et al, 2013;Kakar et al, 2014). In some cases, bicontrol agents may present broad antagonism toward plant pathogens, whereas in others, inhibitory effects could be pathogen-specific, or even specific to environmental or regional physico-chemical soil conditions.…”

Selecting lipopeptide‐producing, Fusarium‐suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10.5

“…To determine the production of biosurfactant compounds, a modified “drop collapse test,” applied according to Yanes, Fuente, Altier, and Arias (), was conducted. Briefly, each well of a 96‐well plate lid coated with 2 μl of commercial test substances consisting of vegetable oil, motor engine oil, kerosene, hexadecane, and paraffin oil was equilibrated for 2 hr.…”

“…To determine the production of biosurfactant compounds, a modified "drop collapse test," applied according to Yanes, Fuente, Altier, and Arias (2012) Belgacem et al (2015). For the microplate assay, 100 μl supernatant was pipetted into a well of 96 microplate and the plate was viewed using a backing sheet of white paper with black grid.…”

Biological control of eucalyptus bacterial wilt with rhizobacteria

“…Many studies have been conducted to explore the biocontrol capacity of these organisms, and their capacity to produce antibiotics makes them a target for the biological control of plant diseases (Raaijmakers and Mazzola, 2012;Yanes et al, 2012;Raaijmakers et al, 2002). Few studies in the world have been conducted to assess the use of rhizobacteria as biological control agent of eucalyptus bacterial wilt, none in Brazil.…”