Characterization of nasal obstruction in the allergic guinea pig using the forced oscillation method

McLeod, Robbie L.; Young, Simon S.; Erickson, Christine H.; Parra, Leonard E.; Hey, John A.

doi:10.1016/s1056-8719(03)00044-3

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…6 The ability to reliably evaluate changes in nasal cavity patency in animals is of paramount importance in the study of pathophysiology and the preclinical evaluation of novel treatments for allergic rhinitis. So far, small animals such as rat 7 and guinea pig, 8 or larger animals such as cat 9 and dog, 10 have been used to generate important information on nasal physiology and pathophysiology. Dynamic techniques require invasive procedures, and the experimental animal cannot survive.…”

mentioning

confidence: 99%

Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis

Nakaya

Dohi

Okunishi

et al. 2006

Laboratory Investigation

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Until now there has been no method for physiologically evaluating nasal hypersensitivity in mice. Enhanced pause (Penh) has been used as an indicator that reflects changes in the lower airway. Recently, however, there is disagreement regarding the significance of the Penh system; this is because Penh is not essentially a physiological parameter, and it might not necessarily represent a change in the lower respiratory tract. The purpose of the present study is to investigate whether Penh could be applicable for analyzing nasal hypersensitivity in mice. BALB/c mice were sensitized with ovalbumin (OVA) through a combination of intraperitoneal injection and daily intranasal challenge in an awake condition. Penh was measured at each time point during sensitization, or a serial change in Penh value was followed after the final nasal challenge and the effect of treatment was assessed. Following sensitization and nasal challenge, the Penh value gradually increased and showed a significant difference on day 14. Changes in IgE, eosinophil infiltration into nasal mucosa, and OVA-induced symptoms all strongly correlated with the increase in Penh. On day 19, after OVA nasal provocation, Penh gradually increased and reached maximal values 25 min after the challenge. Pretreatment with dexamethasone or a histamine H1 blocker significantly suppressed this increase in Penh. We confirmed that intranasal OVA challenge did not induce bronchoconstriction by measuring airway resistance and bronchoalveolar lavage fluid, and through histological examination. These results clearly demonstrate that Penh could be a useful noninvasive indicator for studying nasal hypersensitivity.

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confidence: 99%

Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis

Nakaya

Dohi

Okunishi

et al. 2006

Laboratory Investigation

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“…For example, in guinea pigs, allergen-induced nasal resistance was reversed by antihistamines, but not by an adrenergic agonist (McLeod et al 2002). These results disagree with the ameliorative effects of commonly used vasoconstrictors in humans.…”

Section: Nasal Cavitymentioning

confidence: 80%

From Mouse to Man: Translational Value of Animal Models of Allergic Rhinitis

Wagner¹,

Harkema²

2012

Allergic Rhinitis

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“…For example, in guinea pigs, allergen-induced nasal resistance was reversed by antihistamines, but not by an adrenergic agonist [29]. These results disagree with the ameliorative effects of commonly used vasoconstrictors in humans.…”

Section: Nasal Obstructionmentioning

confidence: 80%

Rodent models of allergic rhinitis: Relevance to human pathophysiology

Wagner

Harkema²

2007

Curr Allergy Asthma Rep

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Rodent models of allergic airways disease employ a wide range of test allergens, sensitization and provocation protocols, animal strains, and experimental endpoints. Studies of experimental asthma, especially the use of murine models, have contributed significantly to the understanding of the genetics and immune-mediated pathophysiology of pulmonary airways during allergy. By comparison, rodent models of allergic rhinitis are less well developed. Recent interest in the potential mechanistic links between asthma and allergic rhinitis has increased the need for relevant animal studies directed at upper airways responses. Specifically, the nature of nasal airway remodeling in response to chronic activation of allergic pathways and its relationship to airway occlusion is not well described. This cursory review discusses current approaches to assessing nasal obstruction in rodent models, and how the histopathologic analysis might be improved to facilitate understanding of the pathogenesis of allergic rhinitis in humans.

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Characterization of nasal obstruction in the allergic guinea pig using the forced oscillation method

Cited by 10 publications

References 35 publications

Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis

Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis

From Mouse to Man: Translational Value of Animal Models of Allergic Rhinitis

Rodent models of allergic rhinitis: Relevance to human pathophysiology

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