Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia

Chan, Kok‐Gan; Atkinson, Steve; Mathee, Kalai; Sam, Choon‐Kook; Chhabra, Siri Ram; Cámara, Miguel; Koh, Chong Lek; Williams, Paul

doi:10.1186/1471-2180-11-51

Cited by 181 publications

(123 citation statements)

References 42 publications

(76 reference statements)

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“…Although AHL lactonase activity has been detected in Acinetobacter sp. strain GG2, isolated from the rhizosphere of ginger (13), no reports of the cloning of AHL acylase genes from the genus Acinetobacter have been published. In this study, we report the cloning and characterization of a gene that encodes a novel AHL acylase from Acinetobacter sp.…”

mentioning

confidence: 99%

AmiE, a Novel N -Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24

Ochiai

Yasumoto

Morohoshi

et al. 2014

Appl Environ Microbiol

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Many Gram-negative bacteria use N-acyl-L-homoserine lactones (AHLs) as quorum-sensing signal molecules. We have reported that Acinetobacter strains isolated from activated sludge have AHL-degrading activity. In this study, we cloned the amiE gene as an AHL-degradative gene from the genomic library of Acinetobacter sp. strain Ooi24. High-performance liquid chromatography analysis revealed that AmiE functions as an AHL acylase, which hydrolyzes the amide bond of AHL. AmiE showed a high level of degrading activity against AHLs with long acyl chains but no activity against AHLs with acyl chains shorter than eight carbons. AmiE showed homology with a member of the amidases (EC 3.5.1.4) but not with any known AHL acylase enzymes. An amino acid sequence of AmiE from Ooi24 showed greater than 99% identities with uncharacterized proteins from Acinetobacter ursingii CIP 107286 and Acinetobacter sp. strain CIP 102129, but it was not found in the draft or complete genome sequences of other Acinetobacter strains. The presence of transposase-like genes around the amiE genes of these three Acinetobacter strains suggests that amiE is transferred by a putative transposon. Furthermore, the expression of AmiE in Pseudomonas aeruginosa PAO1 reduced AHL accumulation and elastase activity, which were regulated by AHL-mediated quorum sensing.

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mentioning

confidence: 99%

AmiE, a Novel N -Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24

Ochiai

Yasumoto

Morohoshi

et al. 2014

Appl Environ Microbiol

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“…The bacteria have high resistance to some heavy metals such as copper, zinc, cadmium, mercury, and lead might be due to the selective pressure by pollution of industrial wastewater at Rungkut, the location from where the strain has been isolated. Chan et al [18] reported that Acinetobacter can ussually isolated from polluted environment. Acinetobacter species are important biotechnological tools, and have been utilized extensively in the synthesis of enzymes and other life-sustaining macromolecules and for degradation of recalcitrant compounds [18].…”

Section: Bacterial Inoculummentioning

confidence: 99%

“…Chan et al [18] reported that Acinetobacter can ussually isolated from polluted environment. Acinetobacter species are important biotechnological tools, and have been utilized extensively in the synthesis of enzymes and other life-sustaining macromolecules and for degradation of recalcitrant compounds [18]. Genus Acinetobacter was a member of bacterial community in rhizosphere of E. crassipes and in wetland water samples [19].…”

Section: Bacterial Inoculummentioning

confidence: 99%

The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

Irawati

Parhusip²,

Sopiah³

et al. 2017

KLS

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Phytoremediation is a bioremediation process using plants and microorganisms to extract, sequester, or detoxify heavy metals. Eichhornia crassipes [(Mart.) Solms] is a well-known phytoremediating plant that has the ability to remove heavy metals from water by accumulating them in their tissues. Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2 are copper resistant bacteria isolated from industrial waste in Rungkut, Surabaya. The aim of this research was to study the effect of Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2 inoculation in copper phytoremediation process using Eichhornia crassipes. Bacterial isolate with colony form unit of 10 8 was inoculated into the rhizosphere of Eichhornia crassipes in water containing 10 mL · L −1 and 20 mL · L

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“…Interruption of QS, also known as quorum quenching, can be achieved by targeting the signalling molecule itself (Chan et al, 2010(Chan et al, , 2011) the transcriptional activator (Manefield et al, 1999) or the autoinducer synthase (Parsek et al, 1999). Thus, anti-QS treatment may be a plausible way to attenuate bacterial virulence without killing the pathogens, thus potentially preventing the acquisition of drug resistance by the bacteria (Adonizio et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa by human serum paraoxonase

Aybey

Demirkan

2016

Journal of Medical Microbiology

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The role of quorum sensing (QS) in the regulation of virulence factor production in Pseudomonas aeruginosa is well established. Increased antibiotic resistance in this bacterium has led to the search for new treatment options, and inhibition of the QS system has been explored for potential therapeutic benefits. If the use of QS inhibitory agents were to lead to a reduction in bacterial virulence, new approaches in the treatment of P. aeruginosa infections could be further developed. Accordingly, we examined whether human serum paraoxonase 1 (hPON1), which uses lactonase activity to hydrolyse N-acyl homoserine lactones, could cleave P. aeruginosa-derived signalling molecules. hPON1 was purified using ammonium sulfate precipitation and hydrophobic interaction chromatography (Sepharose 4B-L-tyrosine-1-naphthylamine). Different concentrations of hPON1 were found to reduce various virulence factors including pyocyanin, rhamnolipid, elastase, staphylolytic LasA protease and alkaline protease. Although treatment with 0.1-10 mg hPON1 ml 21 did not show a highly inhibitory effect on elastase and staphylolytic LasA protease production, it resulted in good inhibitory effects on alkaline protease production at concentrations as low as 0.1 mg ml 21. hPON1 also reduced the production of pyocyanin and rhamnolipid at a concentration of 1.25 mg ml 21(within a range of 0.312-5 mg ml 21). In addition, rhamnolipid, an effective biosurfactant reported to stimulate the biodegradation of hydrocarbons, was able to degrade oil only in the absence of hPON1. INTRODUCTIONQuorum sensing (QS) is a process of bacterial communication used to collectively control group behaviours (Ng & Bassler, 2009;Rutherford & Bassler, 2012). Pseudomonas aeruginosa perceives its local population density using a QS system: as the concentration of the signalling molecules passes a given threshold, the bacteria regulate production of certain virulence factors. To orchestrate the synchronous production of virulence factors, P. aeruginosa relies on two major LuxI/R QS systems: the Las and Rhl systems (Pesci et al., 1997;Schuster et al., 2003). The LuxI homologues LasI and RhlI are responsible for synthesis of the Las and Rhl signals, N-(3-oxododecanoyl)-L-homoserine lactone (PAI1) and N-butanoyl-L-homoserine lactone (PAI2), respectively. PAI1 activates the LuxR-type transcription factor LasR, and, in turn, LasR-PAI1 induces the production of LasB elastase, LasA protease, alkaline protease and LasRI (Gambello et al., , 1993Seed et al., 1995). PAI2 cooperates with the cognate regulator RhlR to enhance the production of rhamnolipid, pyocyanin and LasB elastase (Latifi et al., 1995;Pearson et al., 1995). Expression of many of the virulence factors in P. aeruginosa is controlled by the QS system (Venturi, 2006). Different strains of P. aeruginosa secrete several extracellular proteolytic enzymes that have been implicated as virulence factors. These enzymes include protease IV, alkaline protease, and LasA and LasB elastases (Caballero et al., 2001). These proteases pr...

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Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia

Cited by 181 publications

References 42 publications

AmiE, a Novel N -Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24

AmiE, a Novel N -Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24

The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

Inhibition of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa by human serum paraoxonase

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