Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits

Xu, Tieshan; Gu, Lihong; Zhang, X H; Ye, B G; Liu, X L; Hou, Shuqin

doi:10.4238/2013.february.28.18

Cited by 14 publications

(12 citation statements)

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“…Furthermore, Xu et al . () studied polymorphisms in Pekin duck and identified three significant variations. The first is a c.129T>C subsitution located in the open reading frame and revealed an association with breast muscle thickness.…”

Section: Myostatin Polymorphisms In Poultrymentioning

confidence: 99%

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

2018

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Myostatin, also known as growth differentiation factor 8, a member of the transforming growth factor-beta super-family, is a negative regulator of muscle development. Myostatin acts at key points during pre- and post-natal life of amniotes that ultimately determine the overall muscle mass of an animal. Mutations have already demonstrated the impact of attenuating myostatin activity on muscle development. A number of large animals, including cattle, sheep, dogs and humans, display the 'double muscled' phenotype due to mutations in the myostatin gene. Here, we firstly give an overview of the molecular pathways regulated by myostatin that control muscle development. Then we describe the natural mutations and their associated phenotypes as well as the physiological influence of altering myostatin expression in livestock animals (cattle, sheep, goat, horse, pig, rabbit and chicken). Knowledge of null alleles and polymorphisms in the myostatin gene are of great interest in the animal breeding field, and it could be utilized to improve meat production in livestock animals.

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Section: Myostatin Polymorphisms In Poultrymentioning

confidence: 99%

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

2018

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“…Five polymorphisms at the 5′ and 3′ promoter sites of the MSTN gene were shown to influence bone growth and fat metabolism [34]. In ducks of Pekin breed, the 129T>C polymorphism was associated with the thickness of the pectoral muscle [19]. In studies carried out by Zhong [20], 6 polymorphisms were…”

Section: Discussionmentioning

confidence: 99%

“…In ducks of Pekin breed, the 129T>C polymorphism was associated with the thickness of the pectoral muscle [19]. In studies carried out by Zhong [20], 6 polymorphisms were identified in the 3 exons of the MSTN gene in Sansui ducks, of which g.106>A was associated with slaughter traits.…”

Section: Discussionmentioning

confidence: 99%

“…The mutations identified in the MSTN gene were also associated with race outcomes of dogs [16] and horses [18]. Xu et al [19] identified three SNP polymorphisms in the MSTN gene but only two were associated with breast muscle traits in Pekin duck. The T129C SNP had a significant association with breast muscle thickness, and the second one T952C had a significant association with the fossilia ossis mastoid length.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphism of the Myostatin (MSTN) Gene in Landes and Kielecka Geese Breeds

Smołucha

Kozubska‐Sobocińska

Koseniuk

et al. 2019

Animals

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Simple Summary: Identification of mutations in the myostatin gene, affecting the occurrence of the double muscling phenotype in some breeds of beef cattle, was an impetus for further analysis and identification of mutations within this gene in other animal breeds, characterized by increased meat performance parameters. The number of geese in poultry livestock production in Poland is small. The native geese breeds can be successfully used to produce high-quality poultry meat and can be a very good source of goose meat production for regional and organic products. The aim of the study was to identify a mutation in the MSTN (Myostatin) gene and investigate whether this polymorphism can affect body weight in different periods of life in Landes and Kielecka breeds. Measurements of the examined trait were taken with time intervals to demonstrate the putative effect of the identified SNP (Single Nucleotide Polymorphism) on body weight over the course of bird growth. In conclusion, the identified c.1231C>T polymorphism suggests a possible link between the polymorphism and the BW (body weight) of Kielecka geese in the 12th week of life. The most significant factors affecting the BW values in geese are breed and sex.Abstract: Myostatin, also known as growth differentiation factor 8 (GDF8), belongs to the TGF-β superfamily of proteins. MSTN is a highly conserved protein that acts as a negative regulator of skeletal muscle growth. Loss of myostatin functionality causes the phenotype to appear in the form of 'double musculature', among others in cattle, sheep, and house mice. The presented results of the research were carried out on two geese breeds-Landes and Kielecka. The aim of the study was to identify mutations in the MSTN gene and study their impact on body weight in both geese breeds in different periods of life. Analysis of the obtained results showed the existence of polymorphism in exon 3 (c.1231C>T) and suggested a possible association (p < 0.05) between BW and genotype in 12 weeks of life in male Kielecka geese breed. The identified polymorphism may be one of the factors important for improving body weight in the studied Kielecka breed, therefore, it is necessary to conduct further research on a larger population of geese breeds in order to more accurately estimate the effect of the identified SNP c.1231C>T on BW in geese.

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“…MSTN, ACB98643.1 MSTN cDNA was correctly obtained from P. fucata. As MSTN plays a pivotal role in muscle development, MSTN cDNAs have been cloned from representatives of several different vertebrate and invertebrate groups (Chen et al 2007;Ye et al 2007a;Covi et al 2008;Qian et al 2013;Xu et al 2013;Singh et al 2014). The results of previous studies clearly demonstrate several major differences in MSTN between these different groups.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Single Nucleotide Polymorphisms Identification of Myostatin cDNA in the Pearl Oyster, Pinctada fucata

Qin

Huang

et al. 2014

J World Aquaculture Soc

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Myostatin (MSTN or growth differentiation factor‐8) is considered a negative regulator of muscle growth and development. In this study, we cloned and characterized the full‐length MSTN cDNA from Pinctada fucata, and named it as the Pf‐MSTN cDNA. The single nucleotide polymorphisms (SNPs) in Pf‐MSTN cDNA were then screened and genotyped. The full‐length Pf‐MSTN cDNA was 2644 bp, including an open reading frame of 1248 bp encoding 415 amino acids which contained typical structural characteristics shared by all members of the transforming growth factor‐β (TGF‐β) superfamily including an N‐terminal signal peptide, a propeptide domain, and a TGF‐β superfamily bioactive domain. The Pf‐MSTN mRNA was detected in all tested tissues, with the highest mRNA levels observed in the adductor muscle, indicating that Pf‐MSTN may play a major role in this tissue. Furthermore, by sequencing and alignment, 32 SNP loci were identified in Pf‐MSTN cDNA. Genotyping 50 individuals from a common breeding stock revealed that 21 of these 32 loci were polymorphic. The minor allele frequency was in the range of 0.0400–0.4800, and the polymorphism information content value varied from 0.0739 to 0.3750. The observed and expected heterozygosity ranged from 0.0200 to 1.0000 and from 0.0776 to 0.5051, respectively. These SNPs identified in Pf‐MSTN will be useful for future studies investigating their utility in marker‐assisted selection for P. fucata breeding.

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Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits

Cited by 14 publications

References 0 publications

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

Polymorphism of the Myostatin (MSTN) Gene in Landes and Kielecka Geese Breeds

Molecular Cloning and Single Nucleotide Polymorphisms Identification of Myostatin cDNA in the Pearl Oyster, Pinctada fucata

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