Characterization of Mycoplasma Strains Isolated from House Musk Shrews (<I>Suncus murinus</I>) and Their Infectivity in Mice and Rats

Morphological and Sequence Analysis of Mycoplasma sp. Isolated from the Oral Cavity of a House Musk Shrew (Suncus murinus)

“…Any other mycoplasmas, M. caviae from guinea pig [7], M. cricetuli from Chinese hamster [6], M. felis from cat [15] and M. hyopneumoniae were isolated from pig [10] as well. From the shrew, M. alvi from common shrew (Sorex araneus) was reported by Gourlay in 1976 [4], and in 1993 we isolated Mycoplasma species (strain G666-16R) from the house musk shrew (SUN line) which is hybrid line of three origins, Okinawa, Nagasaki and Jakarta [3]. Recently we isolated Mycoplasma species from EDS line of house muck shrew which is originated from Bangladesh [12].…”

“…The 16S rRNA gene sequence of strain EDS-4 is available through the DDBJ under accession number (AB469852). Gourlay in 1976 [4], and in 1993 we isolated Mycoplasma species (strain G666-16R) from the house musk shrew (SUN line) which is hybrid line of three origins, Okinawa, Nagasaki and Jakarta [3]. Recently we isolated Mycoplasma species from EDS line of house muck shrew which is originated from Bangladesh [12].…”

“…e-mail: gotok@med.teikyo-u.ac.jp database using the DDBJ (DNA Data Bank of Japan; http:// www.ddby.nig.ac.jp/index-e.html) "BLAST" homology search system. Serological identification was assayed by a standard disk growth inhibition technique using antiserum against mycoplasma (strain G666-16R) previously isolated from different origin (SUN line) of Suncus murinus [3]. For serological tests, hyperimmune rabbit antisera to the strain G666-16R were prepared.…”

Rapid Identification of <i>Mycoplasma pulmonis</i> Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

“…For diagnosis of M. pulmonis infection, serological testing, culture methods, and polymerase chain reaction (PCR) assays have been used [3]. The culture method is one of the most common and specific methods to detect bacteria; however, a laborious step is needed to identify the colonies [4], such as morphological studies, reversion studies, or the sterol requirement and metabolism inhibition test. Recently, sequence analysis of the 16S rRNA gene has also been used as a simple method for identification of the bacteria [16].…”

“…pulmonis (strain m53) [9], M. arthritidis (PG6, ATCC19611), and M. neurolyticum (type A, ATCC19988) were used to construct a database for MALDI-TOF MS. Media for cultivation of Mycoplasma strains have been described previously [4]. Briefly, an agar medium containing 70 ml of PPLO broth (BD, Tokyo, Japan) supplemented with 10 ml of 25% (w/v) yeast extract, 20 ml of heat-inactivated horse serum (Life Technologies Japan Ltd., Tokyo, Japan), 1 ml of 100,000 units of penicillin G, 1 ml of 2.5% thallium acetate, and 1.3% Bacto agar (BD) was used.…”