Characterization of Mycobacteria from a Major Brazilian Outbreak Suggests that Revision of the Taxonomic Status of Members of the
            <i>Mycobacterium chelonae</i>
            -
            <i>M. abscessus</i>
            Group Is Needed

Leao, Sylvia Cardoso; Tortoli, Enrico; Viana-Niero, Cristina; Ueki, Suely Yoko Mizuka; Lima, Karla Valéria Batista; Lopes, Maria Luíza; Yubero, Jesús; Menéndez, M. Carmen; García, María Jesús García

doi:10.1128/jcm.00808-09

Cited by 121 publications

(103 citation statements)

References 38 publications

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“…Adicionalmente, é importante ressaltar a nova proposta de reclassificação taxonômica das espécies M. massiliense e M. bolletii como subespécies de M. abscessus, devido à alta similaridade fenotípica e genética entre esses micro-organismos 16 .…”

Section: Perspectivasunclassified

Infecções por micobactérias de crescimento rápido resistentes a desinfetantes: uma problemática nacional?

Pitombo¹,

Lupi²,

Duarte³

2009

Rev. Bras. Ginecol. Obstet.

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Section: Perspectivasunclassified

Infecções por micobactérias de crescimento rápido resistentes a desinfetantes: uma problemática nacional?

Pitombo¹,

Lupi²,

Duarte³

2009

Rev. Bras. Ginecol. Obstet.

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“…Beyond the molecular techniques, the sequencing of the specifi c genes rpoB and hsp65 has become the gold standard for identifying mycobacteria 18,19 . Because of its high discriminatory power, this tool has been used by several groups in epidemiological studies to identify the species involved in human diseases and in outbreaks, and also for taxonomic reclassifi cation 20,21 .…”

Section: Methodsmentioning

confidence: 99%

Rapid detection and differentiation of mycobacterial species using a multiplex PCR system

Lima

Duarte

Montenegro

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods: The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by nontuberculous mycobacteria (NTM) Results: Forty mycobacterial strains isolated from pulmonary and extrapulmonary origin specimens from 37 patients diagnosed with tuberculosis were processed. Using phenotypic and biochemical characteristics of the 40 mycobacteria isolated in LJ medium, 57.5% (n=23) were characterized as the Mycobacterium tuberculosis complex (MTBC) and 20% (n=8) as nontuberculous mycobacteria (NTM), with 22.5% (n=9) of the results being inconclusive. When the results of the phenotypic and biochemical tests in 30 strains of mycobacteria were compared with the results of the multiplex PCR, there was 100% concordance in the identifi cation of the MTBC and NTM species, respectively. A total of 32.5% (n=13) of the samples in multiplex PCR exhibited a molecular pattern consistent with NTM, thus disagreeing with the fi nal diagnosis from the attending physician. Conclusions: Multiplex PCR can be used as a differential method for determining TB infections caused by NTM a valuable tool in reducing the time necessary to make clinical diagnoses and begin treatment. It is also useful for identifying species that were previously not identifi able using conventional biochemical and phenotypic techniques.

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“…Among pulmonary M. abscessus group isolates, almost half of the isolates in Korea and 30% of those in the Netherlands are M. massiliense (8,21). It has been suggested that M. massiliense should be reclassified taxonomically as a subspecies of M. abscessus (11). The clinical significance of differentiating these two species has also been explored (7).…”

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confidence: 99%

Multiple Cases of Cutaneous Mycobacterium massiliense Infection in a “Hot Spa” in Japan

et al. 2011

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Seven body polishers working in the same "hot spa" presented with multiple red nodules and papules on their hands and forearms. A causative agent was successfully isolated from two of the subjects and from a swab sample collected from the underside of a bed cover in the body-polishing facility. The two cutaneous isolates and the environmental isolate were rapidly growing mycobacteria that formed nonphotochromogenic smooth or smooth/rough colonies on Ogawa egg slants. They were identified as Mycobacterium massiliense by multigenotypic analysis using the 16S rRNA, hsp65, and rpoB genes and the 16S-23S rRNA internal transcribed spacer (ITS) region. However, the use of the 16S rRNA gene sequence and/or DNA-DNA hybridization (DDH Mycobacteria Kit) alone would not distinguish M. massiliense from mycobacteria in the M. chelonae-M. abscessus group. The three isolates were significantly more susceptible to clarithromycin, doxycycline, and minocycline than the M. abscessus and M. bolletii reference strains. One cutaneous isolate and the environmental isolate were in a related cluster by randomly amplified polymorphic DNA PCR (RAPD-PCR). Of the several mycobacterial species found in the day spa, only M. massiliense was isolated from biopsy specimens of the skin lesions, suggesting that this bacterium is a human skin pathogen. This is the first known report of cutaneous M. massiliense infections that could not be attributed to a prior invasive procedure. This is also the first report of M. massiliense infection in Japan.Mycobacterium massiliense was initially isolated from the sputum of a patient with pneumonia in France in 2004 (1). Epidemiologically, M. massiliense has been recognized as an emerging pathogen in the United States (16,24) and Brazil, where outbreaks have been associated with postsurgical and cosmetic procedures (2, 4, 22). In Korea, an outbreak was linked to intramuscular injections of an antimicrobial agent (9). This bacterium was also the source of a lethal case of sepsis in Italy and has been found in cystic fibrosis patients in France (15,20). Among pulmonary M. abscessus group isolates, almost half of the isolates in Korea and 30% of those in the Netherlands are M. massiliense (8,21). It has been suggested that M. massiliense should be reclassified taxonomically as a subspecies of M. abscessus (11). The clinical significance of differentiating these two species has also been explored (7). However, M. massiliense has not been fully characterized. Although mycobacteria are a frequent source of dermal infection, M. massiliense has never been reported as an etiological agent. This report describes the first case of an M. massiliense dermal infection in Japan. Case ReportsIn November 2007, a 49-year-old female who worked as a body polisher in a hot spa developed multiple red nodules and papules on her hands and forearms. The number of lesions gradually increased over several months, precipitating a visit to a local hospital in June 2008 (case 1). A skin biopsy specimen of a nodule stained with hematoxylin a...

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Characterization of Mycobacteria from a Major Brazilian Outbreak Suggests that Revision of the Taxonomic Status of Members of the Mycobacterium chelonae - M. abscessus Group Is Needed

Cited by 121 publications

References 38 publications

Infecções por micobactérias de crescimento rápido resistentes a desinfetantes: uma problemática nacional?

Infecções por micobactérias de crescimento rápido resistentes a desinfetantes: uma problemática nacional?

Rapid detection and differentiation of mycobacterial species using a multiplex PCR system

Multiple Cases of Cutaneous Mycobacterium massiliense Infection in a “Hot Spa” in Japan

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