Characterization of Murine Lymphokine-Activated Killer Cell Cultures Separated According to Cell Size

Chadwick, Brian S.; Brady, Gerard; Miller, Richard G.

doi:10.1006/cimm.1993.1001

Cited by 3 publications

(1 citation statement)

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“…In addition to aiding the study of tumours and leukaemia, single-cell pro®ling approaches have been particularly useful in examining haemopoiesis, mRNA expression at the single cell level early embryonic differentiation and cell physiology. As well as using antibody-based fractionation protocols to enrich for de®ned precursors [13,17,41,65,67], the ability to grow haemopoietic precursors in culture, has allowed the identi®cation and molecular characterization of individual cells on the basis of their developmental capacity [9,14,66]. The general principle of this approach (known as sibling analysis; see [9] for details) is to grow individual precursors in vitro under nonrestrictive growth conditions and allow them to divide two or ®ve times to generate a `colony start' of 4±32 cells.…”

Section: Biological Applications Of Single Cell Pro®lingmentioning

confidence: 99%

Expression Profiling of Single Mammalian Cells – Small is Beautiful

Brady

2000

Yeast

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Increasingly mRNA expression patterns established using a variety of molecular technologies such as cDNA microarrays, SAGE and cDNA display are being used to identify potential regulatory genes and as a means of providing valuable insights into the biological status of the starting sample. Until recently, the application of these techniques has been limited to mRNA isolated from millions or, at very best, several thousand cells thereby restricting the study of small samples and complex tissues. To overcome this limitation a variety of amplification approaches have been developed which are capable of broadly evaluating mRNA expression patterns in single cells. This review will describe approaches that have been employed to examine global gene expression patterns either in small numbers of cells or, wherever possible, in actual isolated single cells. The first half of the review will summarize the technical aspects of methods developed for single-cell analysis and the latter half of the review will describe the areas of biological research that have benefited from single-cell expression analysis.

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