Characterization of multi-biomarkers for bone health assessment based on photoacoustic physicochemical analysis method

Abstract

Photoacoustic (PA) techniques are potential alternatives to histopathology. The physicochemical spectrogram (PCS) generated by the PA measurement at multiple wavelengths can presents the morphology and chemical composition target at multi-biomarkers simultaneously. In this work, via multi-wavelength PA measurements performed on rabbit bone models, we investigated the feasibility of using PCSs for bone health assessment. A comprehensive analysis of the PCSs, termed PA physicochemical analysis (PAPCA), was conducted. The “ slope ” and “ relative content ” were used as the PAPCA-quantified parameters to characterize the changes in the physical and chemical properties of bone tissue, respectively. The findings are consistent well with the gold-standard imaging results. It demonstrated that the PAPCA can be used to characterize both the microstructure and content of multi-biomarkers which highly related with bone health. Considering the PA technique is noninvasive and radiation-free, it has great potential in the implementation and monitoring of bone diseases progression.

Development of a semi‐anthropomorphic photoacoustic calcaneus phantom based on nano computed tomography and stereolithography 3D printing

“…The major optical absorbers in the heel within the 690−950 nm operating wavelength range of our PA device are mineral (mostly hydroxyapatite), blood, and lipid. 27 Here, the printing material of the calcaneus bone was permanent crown resin, which has similar optical absorption characteristics with hydroxyapatite. Considering that the fresh blood and lipid, as organic materials, are not stable and deteriorate rapidly, stable substitutes are required.…”

“…We next completed fabrication of the calcaneus phantom by infiltrating the calcaneus model with substitute bone marrow and covering the construct with a soft tissue mimic. The major optical absorbers in the heel within the 690−950 nm operating wavelength range of our PA device are mineral (mostly hydroxyapatite), blood, and lipid 27 . Here, the printing material of the calcaneus bone was permanent crown resin, which has similar optical absorption characteristics with hydroxyapatite.…”

Expeditious detection and segmentation of bone mass variation in DEXA images using the hybrid GLCM-AlexNet approach

“…The suggested joint model was far better than the clinical model alone. Feng et al (2022) proved that a photoacoustic physicochemical analysis could be utilised to find the microstructure and chemical content of bones to determine its health [12]. The slope and the relative content described the variations in the physical and chemical properties of bones.…”

Fluorescent Probes for the Detection of Bone Metabolism

“…DXA is the “gold standard” for clinically diagnosing osteoporosis which can offer the BMD. However, Nevertheless, it falls short in measuring BMA, elasticity, and other significant risk factors that lead to fracture risk [11] . However, traditional imaging methods still face with limited imaging sensitivity, extended capture durations, and certain ionizing radiation and are insufficient to provide real‐time spatial information concerning cellular activity [12] .…”

“…However, Nevertheless, it falls short in measuring BMA, elasticity, and other significant risk factors that lead to fracture risk. [11] However, traditional imaging methods still face with limited imaging sensitivity, extended capture durations, and certain ionizing radiation and are insufficient to provide real-time spatial information concerning cellular activity. [12] In other words, traditional techniques only provide structural information that changes lag behind the actual cellular event and cannot realize the accurate detection of bone metabolism.…”