Characterization of mouse nasal lymphocytes isolated by enzymatic extraction with collagenase

Asanuma, Hideki; Inaba, Yuji; Aizawa, Chikara; Kurata, Takeshi; Tamura, Shinichi

doi:10.1016/0022-1759(95)00165-7

Cited by 62 publications

(42 citation statements)

References 17 publications

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“…We have shown previously that intranasal influenza virus infection induces CD8 + cytotoxic T lymphocytes (CTLs) in nasal lymphocytes around day 7 after infection (Asanuma et al, 1995) which cannot function to prevent infection, but must function to eliminate infected cells (Yap et al, 1978 ;McMichael, 1994). In addition, we have shown that intranasal immunization with adjuvant-combined influenza virus nucleoprotein accelerated virus clearance in the presence of CD4 + T cells, capable of producing IFN-γ and mediating delayed-type hypersensitivity, suggesting that Th1 cells could be involved in virus clearance (Tamura et al, 1996 ;Cher & Mosmann, 1987 ;Mosmann & Coffman, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Non-NALT nasal lymphocytes were prepared from the portion of the nasal cavity (nasal turbinates, septum and lateral walls) remaining after isolation of the NALT according to the method described previously (Asanuma et al, 1995). They probably contained lymphocytes from paired small lymphoid nodules near the periorbital regions, lymphocytes in the lamina propria and intraepithelial lymphocytes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Antibody-forming cells in the nasal-associated lymphoid tissue during primary influenza virus infection.

Tamura

Iwasaki

Thompson

et al. 1998

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antibody-forming cells in the nasal-associated lymphoid tissue during primary influenza virus infection.

Tamura

Iwasaki

Thompson

et al. 1998

Journal of General Virology

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“…Left lung tissue was excised and washed in HBSS. The head of the animal was processed to obtain nasal passages: the facial skin, cheek muscles, bones and palate were removed according to the method of Asanuma et al [3,4]. The remaining sections, consisting of nasal turbinates, septa, and lateral walls were obtained as nasal passage tissue.…”

Section: Experimental Protocolmentioning

confidence: 99%

Parenteral Nutrition Induces Organ Specific Alterations in Polymeric Immunoglobulin Receptor Levels

Sano

Gómez

Hermsen

et al. 2008

Journal of Surgical Research

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Background-Secretory IgA prevents pathogen adherence at mucosal surfaces to prevent infection. Polymeric immunoglobulin receptor (pIgR), located on the basolateral surface of mucosal cells, binds dimeric IgA produced by B cells with the cooperation of T cells in the lamina propria. This IgA-pIgR complex is transported apically, where it is exocytosed as secretory IgA to the mucosal surface. Our prior work shows that parenteral nutrition (PN) impairs both airway and small intestine mucosal immunity by reducing T & B cells and IgA levels. This work examines intestinal and respiratory tissue-specific pIgR responses to PN.

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“…The majority of T cells express the ␣␤ T-cell receptor (␣␤TCR), with few ␥␦TCR ϩ T cells (1). Studies to date suggest that the O-NALT is rich in unswitched, naive B cells and naive T cells, suggesting that it is a mucosal inductive site, whereas the D-NALT may function as an effector site.…”

mentioning

confidence: 99%

Nasal-Associated Lymphoid Tissue Is a Site of Long-Term Virus-Specific Antibody Production following Respiratory Virus Infection of Mice

Liang

Hyland

Hou

2001

J Virol

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Nasal immunoglobulin A provides an initial defense against inhaled respiratory pathogens. However, it is not known whether the nasal-associated lymphoid tissues (NALT) are able to mount an effective long-lasting pathogen-specific immune response, nor is it known whether functional differences exist between the organized NALT (O-NALT) and the diffuse NALT lining the nasal passages (D-NALT). Here we show that although both the O-NALT and the D-NALT are capable of producing virus-specific antibody in response to influenza virus infection, the frequency of specific antibody-forming cells in the D-NALT is much greater than the frequency observed in the O-NALT. Furthermore, we show that the D-NALT but not the O-NALT is the site of long-term virus-specific humoral immunity which lasts for the life of the animal. These results indicate that the D-NALT is not only the major effector site of the NALT but also the site of local long-term specific antibody production.The upper respiratory tract is an important site for host defense against invading pathogens, since it is the site at which inhaled antigens first come into contact with the immune system (9). Respiratory viruses such as influenza virus affect primarily the upper and lower respiratory tracts, and viremia does not normally occur. Intranasal immunization can elicit antigenspecific immune responses in both the mucosal and systemic compartments following administration of pathogens and even nonreplicating protein antigens (4,16,17). Furthermore, intranasal immunization is an effective means of evoking not only local immunity in the respiratory tract but also immunity at distal mucosal sites (10, 15).The nasal-associated lymphoid tissues (NALT) in the mouse are composed of a pair of organized lymphoid aggregates (O-NALT) located on the palate at the entrance to the nasopharyngeal duct and the less well organized diffuse lymphoid tissue lining the nasal passages (D-NALT) (9). These nasal tissues appear to be functionally equivalent to the Waldeyer's ring of tonsils and adenoids in the human and are most likely responsible for the local immune responses generated following intranasal immunization in the mouse (14). An indication of the importance of the nasopharyngeal lymphoid tissue in humans is the diminished poliovirus-specific antibody levels in nasal secretions from children following tonsillectomy (11). In humans resistance to infection with a cold-adapted vaccine influenza virus has been correlated with antihemagglutinin (anti-HA) immunoglobulin A (IgA) in nasal washes (3). In the mouse model, following intranasal infection, local antibodyforming cell (AFC) production in the NALT of BALB/c mice parallels detection of influenza virus-specific antibodies in the nasal wash and correlates with virus clearance from the nose (14). Furthermore, nasal IgA has been shown to directly mediate local anti-influenza virus immunity in the mouse model, confirming the importance of IgA in protection against virus infection in the upper respiratory tract (12). Specific AFCs secr...

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Characterization of mouse nasal lymphocytes isolated by enzymatic extraction with collagenase

Cited by 62 publications

References 17 publications

Antibody-forming cells in the nasal-associated lymphoid tissue during primary influenza virus infection.

Antibody-forming cells in the nasal-associated lymphoid tissue during primary influenza virus infection.

Parenteral Nutrition Induces Organ Specific Alterations in Polymeric Immunoglobulin Receptor Levels

Nasal-Associated Lymphoid Tissue Is a Site of Long-Term Virus-Specific Antibody Production following Respiratory Virus Infection of Mice

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