2005
DOI: 10.1534/genetics.104.038265
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of Mos1-Mediated Mutagenesis in Caenorhabditis elegans

Abstract: Insertional mutagenesis with a heterologous transposon provides a method to rapidly determine the molecular identity of mutated genes. The Drosophila transposon Mos1 can be mobilized to cause mutations in Caenorhabditis elegans (Bessereau et al. 2001); however, the mutagenic rate was initially too low for use in most forward genetic screens. To increase the effectiveness of Mos1-mediated mutagenesis we examined the conditions influencing Mos1 transposition. First, optimal transposition occurs 24 hr after expre… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
46
0

Year Published

2009
2009
2012
2012

Publication Types

Select...
6
1

Relationship

2
5

Authors

Journals

citations
Cited by 43 publications
(46 citation statements)
references
References 23 publications
0
46
0
Order By: Relevance
“…Second, MOS1 is not sufficiently effective to promote a large-scale detectable level of transposition. Results obtained by Williams et al (2005), and Robert and Bessereau (2007) using Mos1 in C. elegans do not support this hypothesis. Third, MOS1 might be down-regulated by post-translational modification, as suggested by the present data.…”
Section: Discussionmentioning
confidence: 85%
“…Second, MOS1 is not sufficiently effective to promote a large-scale detectable level of transposition. Results obtained by Williams et al (2005), and Robert and Bessereau (2007) using Mos1 in C. elegans do not support this hypothesis. Third, MOS1 might be down-regulated by post-translational modification, as suggested by the present data.…”
Section: Discussionmentioning
confidence: 85%
“…Parameters for optimal Mos1 transposition were characterized to optimize the efficiency of Mos1-mediated insertional mutagenesis (Williams et al 2005). Mos1 inserts throughout the C. elegans genome into TA dinucleotides with a slight preference for introns (Granger et al 2004).…”
Section: Custom Knockout and Knock In Alleles By Transgene-instructedmentioning
confidence: 99%
“…When mutagenesis is performed under optimal conditions, Mos1 is only 10 times less mutagenic than the chemical agent EMS (for a detailed protocol see Bessereau 2006a;Boulin and Bessereau 2007). On average, 2.5 Mos1 insertions are recovered per animal (Williams et al 2005). Because of this low copy number of Mos1 insertions per mutant, the mutagenic insertion responsible for a mutant phenotype can be quickly separated from unlinked insertions by out-cross and mapped using inverse PCR.…”
Section: Custom Knockout and Knock In Alleles By Transgene-instructedmentioning
confidence: 99%
“…Mobilization of the exogenous Mos1 transposon, was accomplished in somatic cells and in the germ line of C. elegans (Bessereau et al 2001). The transformation protocol has been optimized since, allowing a significant improvement of the mutagenesis efficiency (Williams et al 2005). Presently the Mos1 system is the most widely used, fully-controllable transposable element for functional genomics purposes in C. elegans (Bessereau 2006).…”
Section: Introductionmentioning
confidence: 99%