Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Nakauchi, Mina; Fukushi, Shuetsu; Saijo, Masayuki; Mizutani, Tetsuya; Ure, Agustín Enrique; Romanowski, Vı́ctor; Kurane, Ichiro; Morikawa, Shigeru

doi:10.1128/cvi.00163-09

Cited by 16 publications

(20 citation statements)

References 24 publications

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“…An ELISA was performed as previously described, except for antigen preparation 13–16. Antigen preparation for the IgG and IgM ELISA was performed as follows.…”

Section: Methodsmentioning

confidence: 99%

“…A Nunc-Immuno Plate (Thermo Fisher Scientific Inc., Waltham, MA) was coated with the predetermined optimal quantity of cell lysates from SFTSV-infected or uninfected Huh7 cells (diluted 1:800 with PBS) and incubated at 4°C overnight. The following procedure was performed in the same way as described previously 13. The cutoff value was set as the average value of the control sera (sera from healthy donors) plus three times the standard deviation (SD = mean + 3 × SD).…”

Section: Methodsmentioning

confidence: 99%

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Family Cluster Analysis of Severe Fever with Thrombocytopenia Syndrome Virus Infection in Korea

Yoo

Heo

Park

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Severe fever with thrombocytopenia syndrome (SFTS) is tick-borne viral disease that was first suspected in China in 2009. The causative virus (SFTSV) was isolated in 2009 and reported in 2011, and SFTSV expanded its geographic distribution in 2012–2013, from China to South Korea and Japan. Most SFTSV infections occur through Haemaphysalis longicornis. However, SFTSV infection can also occur between family members, and nosocomial transmission of SFTSV is also possible through close contact with a patient. In this study, we first analyzed clinical, epidemiological, and laboratory data for SFTS patients and family members of an index patient in Korea. The S segment of SFTSV was amplified from the sera of three patients, and the S segment of SFTSV and IgG specific to SFTSV were detected in the serum from one family member; although this individual had no history of exposure to H. longicornis, she frequently had close contact with the index patient. In Korea, SFTSV infection among family members does not have to be reported, and we suggest that person-to-person transmission of SFTSV among family members is possible in Korea.

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“…An ELISA was performed as previously described, except for antigen preparation 13–16. Antigen preparation for the IgG and IgM ELISA was performed as follows.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Family Cluster Analysis of Severe Fever with Thrombocytopenia Syndrome Virus Infection in Korea

Yoo

Heo

Park

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…Like arenaviruses, the nucleoprotein of bunyaviruses is the most abundant viral antigen present in the virion and in the infected cells [9], [10]. Several serological tests are then often used to detect antibodies against viral nucleoprotein [11]–[15].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies

Bréard

Lara

Comtet³

et al. 2013

PLoS ONE

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A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-flurorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.

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“…Thus, the MAb C11-12 is considered to be a broadly reactive MAb against New World arenaviruses, whereas MAb C6-9 is JUNV-specific. These findings have been confirmed by detailed epitope analyses using peptide mapping [50]. Similarly, the cross-reactivity of MAbs against LASV rNP has been analyzed [51].…”

Section: Recombinant Protein-based Elisa For Detecting Antibodies mentioning

confidence: 82%

Serological Assays Based on Recombinant Viral Proteins for the Diagnosis of Arenavirus Hemorrhagic Fevers

Fukushi

Tani

Yoshikawa

et al. 2012

Viruses

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The family Arenaviridae, genus Arenavirus, consists of two phylogenetically independent groups: Old World (OW) and New World (NW) complexes. The Lassa and Lujo viruses in the OW complex and the Guanarito, Junin, Machupo, Sabia, and Chapare viruses in the NW complex cause viral hemorrhagic fever (VHF) in humans, leading to serious public health concerns. These viruses are also considered potential bioterrorism agents. Therefore, it is of great importance to detect these pathogens rapidly and specifically in order to minimize the risk and scale of arenavirus outbreaks. However, these arenaviruses are classified as BSL-4 pathogens, thus making it difficult to develop diagnostic techniques for these virus infections in institutes without BSL-4 facilities. To overcome these difficulties, antibody detection systems in the form of an enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay were developed using recombinant nucleoproteins (rNPs) derived from these viruses. Furthermore, several antigen-detection assays were developed. For example, novel monoclonal antibodies (mAbs) to the rNPs of Lassa and Junin viruses were generated. Sandwich antigen-capture (Ag-capture) ELISAs using these mAbs as capture antibodies were developed and confirmed to be sensitive and specific for detecting the respective arenavirus NPs. These rNP-based assays were proposed to be useful not only for an etiological diagnosis of VHFs, but also for seroepidemiological studies on VHFs. We recently developed arenavirus neutralization assays using vesicular stomatitis virus (VSV)-based pseudotypes bearing arenavirus recombinant glycoproteins. The goal of this article is to review the recent advances in developing laboratory diagnostic assays based on recombinant viral proteins for the diagnosis of VHFs and epidemiological studies on the VHFs caused by arenaviruses.

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Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Cited by 16 publications

References 24 publications

Family Cluster Analysis of Severe Fever with Thrombocytopenia Syndrome Virus Infection in Korea

Family Cluster Analysis of Severe Fever with Thrombocytopenia Syndrome Virus Infection in Korea

Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies

Serological Assays Based on Recombinant Viral Proteins for the Diagnosis of Arenavirus Hemorrhagic Fevers

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