Characterization of Monoclonal Antibodies against Four Structural Proteins of Rinderpest Virus

Abstract

SUMMARYTwenty-four monoclonal antibodies (MAbs) against rinderpest virus (RPV) were established and characterized by several serological tests. Of the 24 MAbs, 10 recognized the nucleoprotein (NP), six the phosphoprotein (P), four the haemagglutinin (H), and two the fusion (F) protein as determined by radioimmunoprecipitation assay. The specificities of the remaining two MAbs could not be determined. From a competitive binding assay using MAbs against each structural protein, at least five, four and two separate antigenic sites were identified on the NP, P and H proteins, respectively. MAbs against the H protein neutralized the infectivity of the virus, but those against the F protein were only neutralizing in the presence of guinea-pig complement. The reactivities of each of the MAbs for other strains of morbillivirus were tested using an indirect immunofluorescent antibody assay. The MAbs against four out of five antigenic sites on the NP showed cross-reactivity amongst all the strains of morbillivirus tested whereas the fifth antibody reacted only with RPV. Of the antibodies specific for the P protein, the antibody against one site was cross-reactive with all the strains of RPV, measles virus (MV) and canine distemper virus (CDV), the antibody against another site was reactive with RPV and MV but not with CDV, and the antibodies against the other two sites were specific for RPV.

Identification of Immunodominant Neutralizing Epitopes on the Hemagglutinin Protein of Rinderpest Virus

“…When about 80% of an RPV-infected Vero cell monolayer showed cytopathic change, the culture supernatant was collected. The MR mutants in this culture supernatant were cloned by plaque formation as described previously (15). The reactivities of these clones to 19 MAbs against RPV-H were investigated by immunofluorescent antibody (IFA) tests as described previously (15).…”

B95a, a marmoset lymphoblastoid cell line, as a sensitive host for rinderpest virus

“…S-1 and ld were directed to the HA protein; H21,032, R23 and 2-1 were directed to the P protein; M22, Kll, P31, Gll, D12, Bll, and Y1 were directed to the NP protein; and 2a and 7-1 were directed to the F protein (Sugiyama et al, 1989). MAbs A27, A42 and A184 directed to measles virus M protein were also employed (Sato et al, 1985).…”

“…B95a cells equally permissive to all RPV strains were used as host cells. The RPV MAbs employed were reactive with all of two, four and five antigenic sites defined on the HA, P and NP proteins, respectively (Sugiyama et al, 1989). The MAbs directed to the M protein of measles virus reacted with three out of five antigenic sites (Sato et al, 1985).…”

Protective effects of monoclonal antibodies against lethal canine distemper virus infection in mice

“…In some experiments the YSA-TC strain of CDV (Hirayama et al, 1986), the LA strain of rinderpest virus (RPV) and the Edmonston strain of measles virus (MV) (Sugiyama et al, 1989) were also used.…”