Characterization of Monocarboxylate Transporter 1 (MCT1) Binding Affinity for Basigin Gene Products and L1cam

Abstract

The purpose of this study was to determine the binding affinities of Basigin gene products and neural cell adhesion molecule L1cam for monocarboxylate transporter-1 (MCT1). ELISA binding assays were performed in which recombinant proteins of the transmembrane domains of Basigin gene products and L1cam were incubated with MCT1 captured from mouse brain. It was determined that Basigin gene products bind MCT1 with moderate affinity, but L1cam does not bind MCT1. Despite a high degree of sequence conservation between Basigin gene products and L1cam, the sequences are different enough to prevent L1cam from interacting with MCT1.

The Neuroplastin adhesion molecules: key regulators of neuronal plasticity and synaptic function

“…Subsequently, it has been proposed that these learning and memory deficiencies are caused by disruption of a lactate shuttle similar to that in the retina, but between neurones and astrocytes in specific brain regions (Howard et al . ).…”

“…Naruhashi et al (1997) reported abnormalities of sensory and memory functions in homozygous Basigin knockout mice, including impaired performance in a Y-maze task which assesses short-term memory and in a water finding task which examines latent learning without any motor dysfunction. Subsequently, it has been proposed that these learning and memory deficiencies are caused by disruption of a lactate shuttle similar to that in the retina, but between neurones and astrocytes in specific brain regions (Howard et al 2010).…”